Functional Study on the Key Gene LaLBD37 Related to the Lily Bulblets Formation
Abstract
:1. Introduction
2. Results
2.1. Full-Length Cloning and Sequence Analysis of LaLBD37
2.2. Subcellular Localization and Transcriptional Activation of LaLBD37
2.3. Acquisition of Resistant Plants
2.4. Identification of Genetically Modified Plants
2.4.1. GUS Staining Identification
2.4.2. DNA Level Identification
2.4.3. RNA Level Identification
2.5. Morphological Changes in Transgenic Lilies
2.6. Changes in Soluble Sugar, Starch Content, and Sucrose Synthase Activity in Transgenic Lines
2.7. Determination of Endogenous Hormone Content in Transgenic Lines
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Gene Cloning and Analysis of LaLBD37
4.3. Subcellular Localization of LaLBD37
4.4. Detection of Transcriptional Autoactivation Activity in LaLBD37
4.5. Agrobacterium Mediated Transformation of Small Scales from Sterile Seedlings of ‘Sorbonne’
4.6. Identification of Genetically Modified Plants
4.6.1. Detection of GUS Activity in Transformed Plants
4.6.2. PCR Detection of Transformed Plants
4.6.3. Real-Time Fluorescence Quantitative Detection of Transformed Plants
4.7. Phenotypic Observation of Transgenic ‘Sorbonne’ Lines
4.8. Metabolite Analysis
4.9. Determination of Endogenous Hormone Content in Transgenic Lines
4.10. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hou, X.; Zhang, K.; Lyu, Y. Functional Study on the Key Gene LaLBD37 Related to the Lily Bulblets Formation. Int. J. Mol. Sci. 2024, 25, 9456. https://doi.org/10.3390/ijms25179456
Hou X, Zhang K, Lyu Y. Functional Study on the Key Gene LaLBD37 Related to the Lily Bulblets Formation. International Journal of Molecular Sciences. 2024; 25(17):9456. https://doi.org/10.3390/ijms25179456
Chicago/Turabian StyleHou, Xinru, Kewen Zhang, and Yingmin Lyu. 2024. "Functional Study on the Key Gene LaLBD37 Related to the Lily Bulblets Formation" International Journal of Molecular Sciences 25, no. 17: 9456. https://doi.org/10.3390/ijms25179456