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Open AccessArticle
Genome-Wide Identification of NAC Family Genes and Their Expression Analyses in Response to Osmotic Stress in Cannabis sativa L
by
Qi Li
Qi Li †,
Hanxue Zhang
Hanxue Zhang †,
Yulei Yang
Yulei Yang ,
Kailei Tang
Kailei Tang
,
Yang Yang
Yang Yang
,
Wenjing Ouyang
Wenjing Ouyang
and
Guanghui Du
Guanghui Du *
School of Agriculture, Yunnan University, Kunming 650500, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(17), 9466; https://doi.org/10.3390/ijms25179466 (registering DOI)
Submission received: 6 August 2024
/
Revised: 25 August 2024
/
Accepted: 28 August 2024
/
Published: 30 August 2024
Abstract
NAC (NAM, ATAF1/2, and CUC2) transcription factors are unique and essential for plant growth and development. Although the NAC gene family has been identified in a wide variety of plants, its chromosomal location and function in Cannabis sativa are still unknown. In this study, a total of 69 putative CsNACs were obtained, and chromosomal location analysis indicated that the CsNAC genes mapped unevenly to 10 chromosomes. Phylogenetic analyses showed that the 69 CsNACs could be divided into six subfamilies. Additionally, the CsNAC genes in group IV-a are specific to Cannabis sativa and contain a relatively large number of exons. Promoter analysis revealed that most CsNAC promoters contained cis-elements related to plant hormones, the light response, and abiotic stress. Furthermore, transcriptome expression profiling revealed that 24 CsNAC genes in two Cannabis sativa cultivars (YM1 and YM7) were significantly differentially expressed under osmotic stress, and these 12 genes presented differential expression patterns across different cultivars according to quantitative real-time PCR (RT–qPCR) analysis. Among these, the genes homologous to the CsNAC18, CsNAC24, and CsNAC61 genes have been proven to be involved in the response to abiotic stress and might be candidate genes for further exploration to determine their functions. The present study provides a comprehensive insight into the sequence characteristics, structural properties, evolutionary relationships, and expression patterns of NAC family genes under osmotic stress in Cannabis sativa and provides a basis for further functional characterization of CsNAC genes under osmotic stress to improve agricultural traits in Cannabis sativa.
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MDPI and ACS Style
Li, Q.; Zhang, H.; Yang, Y.; Tang, K.; Yang, Y.; Ouyang, W.; Du, G.
Genome-Wide Identification of NAC Family Genes and Their Expression Analyses in Response to Osmotic Stress in Cannabis sativa L. Int. J. Mol. Sci. 2024, 25, 9466.
https://doi.org/10.3390/ijms25179466
AMA Style
Li Q, Zhang H, Yang Y, Tang K, Yang Y, Ouyang W, Du G.
Genome-Wide Identification of NAC Family Genes and Their Expression Analyses in Response to Osmotic Stress in Cannabis sativa L. International Journal of Molecular Sciences. 2024; 25(17):9466.
https://doi.org/10.3390/ijms25179466
Chicago/Turabian Style
Li, Qi, Hanxue Zhang, Yulei Yang, Kailei Tang, Yang Yang, Wenjing Ouyang, and Guanghui Du.
2024. "Genome-Wide Identification of NAC Family Genes and Their Expression Analyses in Response to Osmotic Stress in Cannabis sativa L" International Journal of Molecular Sciences 25, no. 17: 9466.
https://doi.org/10.3390/ijms25179466
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