X-ray Radiotherapy Impacts Cardiac Dysfunction by Modulating the Sympathetic Nervous System and Calcium Transients
Abstract
:1. Introduction
2. Results
2.1. Effects of Top-Heart X-ray Irradiation on Atrial and Ventricular Cardiac Function in Mice 60 Weeks Post-IR
2.2. Effect of Top-Heart X-ray Irradiation on Atrial Morphology and Remodeling
2.3. The Top-Heart X-ray Irradiation Induced a Delayed Reorganization of Cardiac SNS
2.4. Effect of Top-Heart X-ray Irradiation on Excitation–Contraction Coupling in Atrial Cardiomyocytes
3. Discussion
4. Materials and Methods
4.1. Animal Experimental Procedures
4.2. Irradiation Procedure
4.3. Ultrasound Doppler and Speckle Tracking Analysis
4.4. Atrial Burst Pacing
4.5. Micro-CT Imaging
4.6. Immunohistological Assessment of Heart Injury
4.7. Whole-Mount Immunolabeling Using Tissue Clearing Method (iDISCO+)
4.8. Western Blot Analysis
4.9. ELISA Catecholamines
4.10. Ca2+ Assay
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Feat-Vetel, J.; Suffee, N.; Bachelot, F.; Dos Santos, M.; Mougenot, N.; Delage, E.; Saliou, F.; Martin, S.; Brunet, I.; Sicard, P.; et al. X-ray Radiotherapy Impacts Cardiac Dysfunction by Modulating the Sympathetic Nervous System and Calcium Transients. Int. J. Mol. Sci. 2024, 25, 9483. https://doi.org/10.3390/ijms25179483
Feat-Vetel J, Suffee N, Bachelot F, Dos Santos M, Mougenot N, Delage E, Saliou F, Martin S, Brunet I, Sicard P, et al. X-ray Radiotherapy Impacts Cardiac Dysfunction by Modulating the Sympathetic Nervous System and Calcium Transients. International Journal of Molecular Sciences. 2024; 25(17):9483. https://doi.org/10.3390/ijms25179483
Chicago/Turabian StyleFeat-Vetel, Justyne, Nadine Suffee, Florence Bachelot, Morgane Dos Santos, Nathalie Mougenot, Elise Delage, Florian Saliou, Sabrina Martin, Isabelle Brunet, Pierre Sicard, and et al. 2024. "X-ray Radiotherapy Impacts Cardiac Dysfunction by Modulating the Sympathetic Nervous System and Calcium Transients" International Journal of Molecular Sciences 25, no. 17: 9483. https://doi.org/10.3390/ijms25179483