Characterization of TBP and TAFs in Mungbean (Vigna radiata L.) and Their Potential Involvement in Abiotic Stress Response
Abstract
:1. Introduction
2. Results
2.1. Characterization of TBP and TAF Genes in Mungbean
2.2. Systematic Analysis of Mungbean TBP and Putative TAFs
2.2.1. VrTBP
2.2.2. VrTAF1
2.2.3. VrTAF2
2.2.4. VrTAF4b
2.2.5. VrTAF5
2.2.6. VrTAF6
2.2.7. VrTAF7
2.2.8. VrTAF8
2.2.9. VrTAF9
2.2.10. VrTAF10
2.2.11. VrTAF11
2.2.12. VrTAF12
2.2.13. VrTAF12b
2.2.14. VrTAF13
2.2.15. VrTAF14b
2.2.16. VrTAF15
2.2.17. VrTAF15b
2.3. VrTBP and VrTAFs Expression in Response to Abiotic Stress Treatments
2.3.1. High-Salinity Stress
2.3.2. Water-Deficit Stress
2.3.3. Heat and Cold Stress
3. Discussion
4. Materials and Methods
4.1. Identification, Structure, and Motif Analyses of VrTBP and VrTAFs
4.2. Phylogenetic Analysis of TBP and TAF Genes from Multi-Species
4.3. Plant Materials
4.4. High-Salinity Treatment
4.5. Water-Deficit Treatment
4.6. Heat and Cold Treatment
4.7. RNA-Seq, Data Statistical Analysis, and Visualization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Gene | Locus ID | NCBI Protein | NCBI mRNA | Location | Length (aa) | pI | M.W. (kDa) | Predicted Cell Localizations |
---|---|---|---|---|---|---|---|---|---|
VrTBP | Vradi0305s00090.1 | LOC106754936 | XP_014492490.1 | XM_014637004.2 | NW_014543812.1 (24061..29626, complement) | 200 | 9.68 | 22.38 | Nucleus |
VrTAF1 | Vradi11g07970.1 | LOC106777544 | X1:XP_014520632.1 X2:XP_014520633.1 X3:XP_014520634.1 X4:XP_022631751.1 | X1:XM_014665146.2 X2:XM_014665147.2 X3:XM_014665148.2 X4:XM_022776030.1 | Chr 11, NC_028361.1 (8353773..8377433) | 1901 1767 1752 1746 | 5.62 6.40 6.98 6.90 | 215.26 200.48 198.72 197.99 | Nucleus |
VrTAF2 | Vradi07g18120.1 | LOC106768217 | X1:XP_014508704.1 X2:XP_022639382.1 X3:XP_022639383.1 | X1:XM_014653218.2 X2:XM_022783661.1 X3:XM_022783662.1 | Chr 7, NC_028357.1 (39566756..39586448) | 1384 1354 939 | 6.23 6.41 6.26 | 156.59 153.22 106.88 | Nucleus |
VrTAF4b | Vradi06g14750.1 | LOC106764661 | X1:XP_014504463.1 X2:XP_014504464.1 X3:XP_014504465.1 X4:XP_014504466.1 X5:XP_014504467.1 X6:XP_014504469.1 X7:XP_022638246.1 | X1:XM_014648977.2 X2:XM_014648978.2 X3:XM_014648979.2 X4:XM_014648980.2 X5:XM_014648981.2 X6:XM_014648983.2 X7:XM_022782525.1 | Chr 6, NC_028356.1 (34643049..34655243) | 936 936 936 936 932 845 845 | 8.38 8.38 8.38 8.38 8.38 9.39 9.39 | 101.74 101.74 101.74 101.74 101.33 92.01 92.01 | Nucleus |
VrTAF5–1 | Vradi06g13500.1 | LOC106765332 | X1:XP_022638193.1 X2:XP_014505399.1 | X1:XM_022782472.1 X2:XM_014649913.2 | Chr 6, NC_028356.1 (32613390..32624024, complement) | 659 658 | 6.37 6.37 | 72.96 72.82 | Nucleus |
VrTAF5–2 | Vradi05g10920.1 | LOC106762344 | XP_014501696.1 | XM_014646210.2 | Chr 5, NC_028355.1 (19815499..19824598, complement) | 671 | 6.30 | 74.27 | Nucleus |
VrTAF6–1 | Vradi04g10770.1 | LOC106758550 | XP_014496955.1 | XM_014641469.2 | Chr 4, NC_028354.1 (19764236..19770172) | 543 | 6.30 | 60.42 | Nucleus |
VrTAF6–2 | Vradi10g02250.1 | LOC106775101 | XP_014517632.1 | XM_014662146.2 | Chr 10, NC_028360.1 (6707871..6712998) | 536 | 7.31 | 59.80 | Nucleus |
VrTAF6–like | / | LOC111240733 | XP_022632040.1 | XM_022776319.1 | NW_014541992.1 (22204..24055) | 192 | 5.49 | 21.37 | Nucleus |
VrTAF7 | Vradi06g12480.1 | LOC106763916 | (1)XP_014503580.1 (2)XP_022638147.1 | (1)XM_014648094.2 (2)XM_022782426.1 | Chr 6, NC_028356.1 (30127567..30130853) | 199 | 4.60 | 22.22 | Nucleus |
VrTAF8–1 | Vradi04g09290.1 | LOC106759260 | XP_022635899.1 | XM_022780178.1 | Chr 4, NC_028354.1 (18214406..18217173) | 406 | 5.55 | 44.92 | Nucleus |
VrTAF8–2 | Vradi06g06010.1 | LOC106764373 | XP_014504145.1 | XM_014648659.2 | Chr 6, NC_028356.1 (7592855..7594847, complement) | 290 | 9.22 | 32.59 | Nucleus |
VrTAF8–like | Vradi08g07170.1 | LOC106769930 | XP_014511223.1 | XM_014655737.2 | Chr 8, NC_028358.1 (18416509..18418289, complement) | 350 | 5.97 | 38.49 | Nucleus |
VrTAF9–1 | Vradi06g02960.1 | LOC106764413 | X1:XP_014504185.1 X2:XP_014504186.1 | X1:XM_014648699.2 X2:XM_014648700.2 | Chr 6, NC_028356.1 (3016606..3019713, complement) | 189 175 | 4.49 4.48 | 21.32 19.68 | Nucleus |
VrTAF9–2 | Vradi07g24390.1 | LOC106767081 | XP_014507387.1 | XM_014651901.2 | Chr 7, NC_028357.1 (47737783..47740700, complement) | 177 | 5.08 | 20.20 | Nucleus |
VrTAF10 | Vradi04g11480.1 | LOC106758746 | (1)XP_014497210.1 (2)XP_014497209.1 | (1)XM_014641724.2 (2)XM_014641723.2 | Chr 4, NC_028354.1 (20649254..20652967, complement) | 136 | 5.16 | 15.26 | Nucleus |
VrTAF11 | Vradi09g05150.1 | LOC106773625 | XP_014515833.1 | XM_014660347.2 | Chr 9, NC_028359.1 (7507755..7510113) | 204 | 7.04 | 23.07 | Nucleus |
VrTAF12 | Vradi08g05550.1 | LOC106772695 | (1)XP_014514733.1 (2)XP_014514734.1 (3)XP_022640289.1 | (1)XM_014659247.2 (2)XM_014659248.2 (3)XM_022784568.1 | Chr 8, NC_028358.1 (12062609..12070416) | 504 | 10.12 | 53.02 | Nucleus |
VrTAF12b | Vradi09g02430.1 | LOC106774141 | XP_014516496.2 | XM_014661010.2 | Chr 9, NC_028359.1 (2520168..2532650) | 1064 | 8.58 | 116.82 | Nucleus |
VrTAF13 | Vradi08g10630.1 | LOC106770840 | (1)XP_014512148.1 (2)XP_022640670.1 (3)XP_022640669.1 (4)XP_014512151.1 (5)XP_014512149.1 (6)XP_014512150.1 | (1)XM_014656662.2 (2)XM_022784949.1 (3)XM_022784948.1 (4)XM_014656665.2 (5)XM_014656663.2 (6)XM_014656664.2 | Chr8, NC_028358.1 (28680758..28685336, complement) | 136 | 5.31 | 15.32 | Nucleus |
VrTAF14b–1 | Vradi11g06360.1 | LOC106777003 | XP_022631586.1 | XM_022775865.1 | Chr11, NC_028361.1 (6305127..6307465) | 279 | 6.07 | 31.27 | Chloroplast, Nucleus |
VrTAF14b–2 | Vradi0393s00050.1 | LOC106780520 | XP_014524308.1 | XM_014668822.2 | NW_014542625.1 (46546..49945) | 273 | 7.17 | 30.73 | Nucleus |
VrTAF15–1 | Vradi02g02560.1 | LOC106777580 | XP_014520706.1 | XM_014665220.2 | Chr2, NC_028352.1 (2396952..2402492, complement) | 390 | 8.28 | 43.21 | Nucleus |
VrTAF15–2 | Vradi05g09940.1 | LOC106760812 | XP_014499728.1 | XM_014644242.2 | Chr5, NC_028355.1 (18244440..18246193) | 156 | 8.78 | 17.08 | Nucleus |
VrTAF15b–1 | Vradi0153s00320.1 | LOC106752587 | XP_014489787.1 | XM_014634301.2 | NW_014542917.1 (751087..753754, complement) | 422 | 9.00 | 43.30 | Chloroplast |
VrTAF15b–2 | Vradi0158s00270.1 | LOC106752498 | XP_022632845.1 | XM_022777124.1 | NW_014542914.1 (178992..183259) | 524 | 8.92 | 51.08 | Nucleus |
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Wu, R.; Jia, Q.; Guo, Y.; Lin, Y.; Liu, J.; Chen, J.; Yan, Q.; Yuan, N.; Xue, C.; Chen, X.; et al. Characterization of TBP and TAFs in Mungbean (Vigna radiata L.) and Their Potential Involvement in Abiotic Stress Response. Int. J. Mol. Sci. 2024, 25, 9558. https://doi.org/10.3390/ijms25179558
Wu R, Jia Q, Guo Y, Lin Y, Liu J, Chen J, Yan Q, Yuan N, Xue C, Chen X, et al. Characterization of TBP and TAFs in Mungbean (Vigna radiata L.) and Their Potential Involvement in Abiotic Stress Response. International Journal of Molecular Sciences. 2024; 25(17):9558. https://doi.org/10.3390/ijms25179558
Chicago/Turabian StyleWu, Ranran, Qiyuan Jia, Yingjian Guo, Yun Lin, Jinyang Liu, Jingbin Chen, Qiang Yan, Na Yuan, Chenchen Xue, Xin Chen, and et al. 2024. "Characterization of TBP and TAFs in Mungbean (Vigna radiata L.) and Their Potential Involvement in Abiotic Stress Response" International Journal of Molecular Sciences 25, no. 17: 9558. https://doi.org/10.3390/ijms25179558