Comparative Analysis of the Mitochondrial Genome of Eggplant (Solanum melongena L.) to Identify Cytoplasmic Male Sterility Candidate Genes
Abstract
:1. Introduction
2. Results
2.1. Sterility Characters and Cytological Observation
2.2. Assembly and Annotation of the Mitochondrial Genome
2.3. Mitochondrial Genome Comparison of 327A and 327B
2.3.1. SNP and InDel Analysis
2.3.2. Structural Variation Analysis
2.4. Analysis of Endemic ORF and Identification of CMS Candidate Genes
2.5. Developing Molecular Markers for CMS
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Paraffin Sections and Cytological Observations
4.3. De Novo Mitochondrial Genome and Assembly
4.4. Gene Prediction and Functional Annotation
4.5. Collinearity and Variation Analysis
4.6. Analysis of Candidate Gene Expression
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Deng, W.; Gan, G.; Li, W.; Yu, C.; Jiang, Y.; Li, D.; Yang, Q.; Li, W.; Wang, P.; Wang, Y. Comparative Analysis of the Mitochondrial Genome of Eggplant (Solanum melongena L.) to Identify Cytoplasmic Male Sterility Candidate Genes. Int. J. Mol. Sci. 2024, 25, 9743. https://doi.org/10.3390/ijms25179743
Deng W, Gan G, Li W, Yu C, Jiang Y, Li D, Yang Q, Li W, Wang P, Wang Y. Comparative Analysis of the Mitochondrial Genome of Eggplant (Solanum melongena L.) to Identify Cytoplasmic Male Sterility Candidate Genes. International Journal of Molecular Sciences. 2024; 25(17):9743. https://doi.org/10.3390/ijms25179743
Chicago/Turabian StyleDeng, Wentao, Guiyun Gan, Weiliu Li, Chuying Yu, Yaqin Jiang, Die Li, Qihong Yang, Wenjia Li, Peng Wang, and Yikui Wang. 2024. "Comparative Analysis of the Mitochondrial Genome of Eggplant (Solanum melongena L.) to Identify Cytoplasmic Male Sterility Candidate Genes" International Journal of Molecular Sciences 25, no. 17: 9743. https://doi.org/10.3390/ijms25179743