A Visual Distance-Based Capillary Immunoassay Using Biomimetic Polymer Nanoparticles for Highly Sensitive and Specific C-Reactive Protein Quantification
Abstract
:1. Introduction
2. Results
2.1. Principle of PMPC NPs-Based Immunoassay for Visual CRP Quantification
2.2. Synthesis and Characterization of the PMPC NPs and Antibody-Functionalized NPs
2.3. Performance of the PMPC NPs for Binding CRP
2.4. Optimization of Experimental Conditions
2.5. Linearity Range and Limit of Detection
2.6. Specificity Experiments
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Instrumentation
4.3. Synthesis and Characterization of PMPC Nanoparticles
4.4. Synthesis and Characterization of Antibody-Conjugated Magnetic Nanoparticles
4.5. Optimization of Incubation Time
4.6. Microscope Imaging of Nanoparticle Complex
4.7. Detection of CRP Concentrations Using PMPC NPs
4.8. Specificity Experiment
4.9. Statisticsusing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Huang, R.; Liu, Z.; Jiang, X.; Huang, J.; Zhou, P.; Mou, Z.; Ma, D.; Cui, X. A Visual Distance-Based Capillary Immunoassay Using Biomimetic Polymer Nanoparticles for Highly Sensitive and Specific C-Reactive Protein Quantification. Int. J. Mol. Sci. 2024, 25, 9771. https://doi.org/10.3390/ijms25189771
Huang R, Liu Z, Jiang X, Huang J, Zhou P, Mou Z, Ma D, Cui X. A Visual Distance-Based Capillary Immunoassay Using Biomimetic Polymer Nanoparticles for Highly Sensitive and Specific C-Reactive Protein Quantification. International Journal of Molecular Sciences. 2024; 25(18):9771. https://doi.org/10.3390/ijms25189771
Chicago/Turabian StyleHuang, Ruodong, Zhenbo Liu, Xinlin Jiang, Junqi Huang, Ping Zhou, Zongxia Mou, Dong Ma, and Xin Cui. 2024. "A Visual Distance-Based Capillary Immunoassay Using Biomimetic Polymer Nanoparticles for Highly Sensitive and Specific C-Reactive Protein Quantification" International Journal of Molecular Sciences 25, no. 18: 9771. https://doi.org/10.3390/ijms25189771