Increased Motility in Campylobacter jejuni and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation
Abstract
:1. Introduction
2. Results
2.1. Changes in the C. jejuni Growth Phenotype after Loss of RsmA Methylation
2.2. Changes in C. jejuni Biofilm Formation after Loss of RsmA Methylation
2.3. Changes in C. jejuni Virulence after Loss of RsmA Methylation
2.4. Upregulated Expression of Proteins Associated with Motility in the C. jejuni rsmA Mutant
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Growth Conditions
4.2. Construction of rsmA Knockout Mutant
Strains | Relevant Characteristics | Source/Reference |
---|---|---|
C. jejuni 81-176 | Wild-type | [53] |
C. jejuni 81-176 ΔrsmA | Cmr, rsmA deletion mutant | This study |
C. jejuni 81-176 ΔrsmA::rsmA | Cmr, Kmr, rsmA deletion mutant complemented with C. jejuni rsmA | This study |
4.3. Motility
4.4. Virulence Phenotypes
4.5. MIC Determination
4.6. Primer Extension
4.7. Competition Growth Assay and Florescence PCR
4.8. Transmission Electron Microscopy (TEM)
4.9. Scanning Electron Microscopy (SEM)
4.10. Protein Extraction and Peptide Labeling
4.11. LC-MS3 Tandem Mass Spectrometry
4.12. Protein Identification and Quantification
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Description | Protein | Gene ID | Abundance Ratio | |
---|---|---|---|---|
ΔrsmA/ WT | ΔrsmA/ ΔrsmA::rsmA | |||
Flagellar hook protein | FlgE | CJJ81176_0025 | 2.1 | 4.2 |
Flagellar protein FlaG | FlaG | CJJ81176_0572 | 2.2 | 1.6 |
Flagellar hook-associated protein | FliD | CJJ81176_0573 | 2.0 | 1.5 |
Flagellar basal-body rod protein | FlgG | CJJ81176_0721 | 2.2 | 6.6 |
Flagellar biosynthesis protein | FlhA | CJJ81176_0890 | 1.8 | 1.3 |
PaaI family thioesterase | Cjj0996 | CJJ81176_0996 | 3.2 | 3.6 |
Putative lipoprotein. | FlgP | CJJ81176_1045 | 4.2 | 4.3 |
Flagellin modification protein | PseA | CJJ81176_1333 | 2.0 | 1.2 |
Flagellin | FlaB | CJJ81176_1338 | 2.1 | 4.3 |
Flagellin | FlaA | CJJ81176_1339 | 2.7 | 3.3 |
30S ribosomal protein S12 | RpsL | CJJ81176_0511 | 2.2 | 1.5 |
Putative imidazole glycerol phosphate synthase subunit | HisF2 | CJJ81176_1331 | 2.1 | 1.4 |
Imidazole glycerol phosphate synthase subunit | HisH1 | CJJ81176_1332 | 2.9 | 1.4 |
Amino acid-binding protein | GlnH | CJJ81176_0836 | 0.5 | 0.4 |
Phosphate ABC transporter. periplasmic phosphate-binding protein | PstS | CJJ81176_0642 | 3.0 | 1.3 |
Thioredoxin family protein | Cjj1656 | CJJ81176_1656 | 1.9 | 1.4 |
Peptidase. M24 family | PepP | CJJ81176_0681 | 1.9 | 1.9 |
Peptidase. M23/M37 family | Cjj1105 | CJJ81176_1105 | 1.8 | 1.3 |
Pseudouridine synthase | RluB | CJJ81176_0003 | 0.4 | 0.7 |
pTet and pVir proteins | ||||
Uncharacterized protein | Cpp33 | CJJ81176_pTet0030 | 2.6 | 1.4 |
Single-stranded DNA-binding protein | Ssb | CJJ81176_pTet0031 | 2.1 | 1.3 |
Uncharacterized protein | Cpp35 | CJJ81176_pTet0032 | 2.1 | 1.3 |
VirB8 | VirB8 | CJJ81176_pVir0001 | 0.5 | 0.6 |
VirB9 | VirB9 | CJJ81176_pVir0002 | 0.5 | 0.6 |
Uncharacterized protein | Cjp07 | CJJ81176_pVir0008 | 0.5 | 0.5 |
Uncharacterized protein | Cjp08 | CJJ81176_pVir0009 | 0.4 | 0.6 |
VirB4 | VirB4 | CJJ81176_pVir0053 | 0.5 | 0.6 |
Uncharacterized protein | Cpp20 | CJJ81176_pTet0015 | 0.4 | 0.6 |
Uncharacterized protein | Cpp47 | CJJ81176_pTet0044 | 0.4 | 0.5 |
Ribosomal RNA small subunit methyltransferase A | RsmA | CJJ81176_0005 | 0.2 | 0.08 |
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Sałamaszyńska-Guz, A.; Murawska, M.; Bącal, P.; Ostrowska, A.; Kwiecień, E.; Stefańska, I.; Douthwaite, S. Increased Motility in Campylobacter jejuni and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation. Int. J. Mol. Sci. 2024, 25, 9797. https://doi.org/10.3390/ijms25189797
Sałamaszyńska-Guz A, Murawska M, Bącal P, Ostrowska A, Kwiecień E, Stefańska I, Douthwaite S. Increased Motility in Campylobacter jejuni and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation. International Journal of Molecular Sciences. 2024; 25(18):9797. https://doi.org/10.3390/ijms25189797
Chicago/Turabian StyleSałamaszyńska-Guz, Agnieszka, Małgorzata Murawska, Paweł Bącal, Agnieszka Ostrowska, Ewelina Kwiecień, Ilona Stefańska, and Stephen Douthwaite. 2024. "Increased Motility in Campylobacter jejuni and Changes in Its Virulence, Fitness, and Morphology Following Protein Expression on Ribosomes with Altered RsmA Methylation" International Journal of Molecular Sciences 25, no. 18: 9797. https://doi.org/10.3390/ijms25189797