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Open AccessArticle
Effects of Alkalinity Stress on Amino Acid Metabolism Profiles and Oxidative-Stress-Mediated Apoptosis/Ferroptosis in Hybrid Sturgeon (Huso dauricus ♀ × Acipenser schrenckii ♂) Livers
by
Cunhua Zhai
Cunhua Zhai 1,2,†,
Xiafei Liu
Xiafei Liu 1,3,†,
Yutao Li
Yutao Li 1,
Ruoyu Wang
Ruoyu Wang
Dr. Ruo Yu Wang obtained a bachelor's degree in Aquaculture from Dalian Ocean University from 2011 a [...]
Dr. Ruo Yu Wang obtained a bachelor's degree in Aquaculture from Dalian Ocean University from September 2011 to June 2015, a master's degree in Aquaculture from Dalian Ocean University from September 2015 to June 2018, and a doctoral degree in Aquaculture from Ningbo University from
September 2018 to June 2023. Since July 2023, he has been working as an associate researcher at the Aquaculture Research Laboratory of the Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences. His research focuses on green and healthy aquaculture techniques for characteristic
freshwater fish and the microecology of aquatic animals.
CunhuaZhai is postgraduate from College of Life Science and Technology, Harbin Normal University. Her research focuses on fisheries resources research and conservation.
1,
Weihua Lv
Weihua Lv 1,
Bo Ma
Bo Ma 1,2,
Dingchen Cao
Dingchen Cao 1 and
Ying Zhang
Ying Zhang 1,3,*
1
Key Laboratory of Cold Water Fish Germplasm Resources and Multiplication and Cultivation of Heilongjiang Province, Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
2
College of Life Science and Technology, Harbin Normal University, Harbin 150025, China
3
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(19), 10456; https://doi.org/10.3390/ijms251910456 (registering DOI)
Submission received: 6 September 2024
/
Revised: 23 September 2024
/
Accepted: 25 September 2024
/
Published: 27 September 2024
Abstract
Alkaline water is toxic to cultured aquatic animals that frequently live in pH-neutral freshwater. Overfishing and habitat destruction have contributed to the decline in the wild sturgeon population; consequently, the domestic hybrid sturgeon has become an increasingly important commercial species in China. Hybrid sturgeons are widely cultured in alkaline water, but little is known about the effects of alkalinity stress on hybrid sturgeon liver tissues. We exposed hybrid sturgeons to four alkaline concentrations (3.14 ± 0.02 mmol/L, 7.57 ± 0.08 mmol/L, 11.78 ± 0.24 mmol/L and 15.46 ± 0.48 mmol/L). Histopathology, biochemical index assessment, gene expression level detection and metabolomics analysis were used to investigate the negative effects on liver functions following exposure to NaHCO3. Livers exposed to alkaline stress exhibited severe tissue injury and clear apoptotic characteristics. With increased exposure concentrations, the hepatic superoxide dismutase, catalase, glutathione peroxidase and alkaline phosphatase activities significantly decreased in a dose-dependent manner. NaHCO3 exposure up-regulated the transcriptional levels of apoptosis/ferroptosis-related genes in livers. Similarly, the expression trends of interleukin-1β and heat shock protein genes also increased in high-alkalinity environments. However, the expression levels of complement protein 3 significantly decreased (p < 0.05). Hepatic untargeted metabolomics revealed the alteration conditions of various metabolites associated with the antioxidant response, the ferroptosis process and amino acid metabolism (such as beta-alanine metabolism; alanine, aspartate and glutamate metabolism; and glycine, serine and threonine metabolism). These data provided evidence that NaHCO3 impaired immune functions and the integrity of hybrid sturgeon liver tissues by mediating oxidative-stress-mediated apoptosis and ferroptosis. Our results shed light on the breeding welfare of domestic hybrid sturgeons and promote the economic development of fisheries in China.
Share and Cite
MDPI and ACS Style
Zhai, C.; Liu, X.; Li, Y.; Wang, R.; Lv, W.; Ma, B.; Cao, D.; Zhang, Y.
Effects of Alkalinity Stress on Amino Acid Metabolism Profiles and Oxidative-Stress-Mediated Apoptosis/Ferroptosis in Hybrid Sturgeon (Huso dauricus ♀ × Acipenser schrenckii ♂) Livers. Int. J. Mol. Sci. 2024, 25, 10456.
https://doi.org/10.3390/ijms251910456
AMA Style
Zhai C, Liu X, Li Y, Wang R, Lv W, Ma B, Cao D, Zhang Y.
Effects of Alkalinity Stress on Amino Acid Metabolism Profiles and Oxidative-Stress-Mediated Apoptosis/Ferroptosis in Hybrid Sturgeon (Huso dauricus ♀ × Acipenser schrenckii ♂) Livers. International Journal of Molecular Sciences. 2024; 25(19):10456.
https://doi.org/10.3390/ijms251910456
Chicago/Turabian Style
Zhai, Cunhua, Xiafei Liu, Yutao Li, Ruoyu Wang, Weihua Lv, Bo Ma, Dingchen Cao, and Ying Zhang.
2024. "Effects of Alkalinity Stress on Amino Acid Metabolism Profiles and Oxidative-Stress-Mediated Apoptosis/Ferroptosis in Hybrid Sturgeon (Huso dauricus ♀ × Acipenser schrenckii ♂) Livers" International Journal of Molecular Sciences 25, no. 19: 10456.
https://doi.org/10.3390/ijms251910456
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