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Open AccessArticle
Hyperbaric Treatment Stimulates Chaperone-Mediated Macroautophagy and Autophagy in the Liver Cells of Healthy Female Rats
by
Agnieszka Pedrycz
Agnieszka Pedrycz 1,
Mariusz Kozakiewicz
Mariusz Kozakiewicz 2,
Mansur Rahnama
Mansur Rahnama 3,
Marek Kos
Marek Kos 4,
Ewelina Grywalska
Ewelina Grywalska 5,*,
Marietta Bracha
Marietta Bracha 6,
Anna Grzywacz
Anna Grzywacz 7 and
Iwona Bojar
Iwona Bojar 8
1
Faculty of Medicine and Health Sciences, University of Applied Sciences in Tarnow, Mickiewicza 8, 33-100 Tarnów, Poland
2
Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Jagiellońska 13/15, 85-067 Bydgoszcz, Poland
3
Departament of Dental Surgery, Medical University of Lublin, Chodźki 6, 20-093 Lublin, Poland
4
Department of Public Health, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland
5
Department of Experimental Immunology, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
6
Department of Geriatrics, Faculty of Health Sciences, Nicolaus Copernicus University in Toruń, Jagiellońska 15, 85-067 Bydgoszcz, Poland
7
Independent Laboratory of Behaviour Genetics and Epigenetics, Pomeranian Medical University in Szczecin, Rybacka 1, 70-204 Szczecin, Poland
8
Department of Women’s Health, Institute of Rural Health, 20-093 Lublin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(19), 10476; https://doi.org/10.3390/ijms251910476 (registering DOI)
Submission received: 3 September 2024
/
Revised: 19 September 2024
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Accepted: 25 September 2024
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Published: 28 September 2024
Abstract
Abstract: The role of autophagy goes far beyond the elimination of damaged cellular components and the quality control of proteins. It also cleanses cells from inclusions, including pathogenic viruses, and provides energy-forming components. The liver, which is an organ with increased metabolism, is made up of cells that are particularly vulnerable to damage. Therefore, detoxification of liver cells in the process of autophagy has become a very important issue clinically. The aim of this study was an immunohistochemical evaluation of proteins activated in rat liver cells at different stages of hyperbaric autophagy. The rats used for the study were randomly divided into six equivalent groups—three control groups and three experimental groups. Animals from the experimental groups were subjected to hyperbaric treatment in a hyperbaric chamber, with a pressure of 1.6 ATA for 120 min. They breathed atmospheric air. Rats were decapitated within 5 or 10 days after removal from the chamber. Immunohistochemical reactions with beclin 1, LC3B, RAB7, and HSC73 proteins were carried out on preparations made from liver slices. A three-step labeled streptavidin–biotin detection method of paraffin blocks (LSAB three-step) was used for immunohistochemical research. The results were evaluated using computer programs for morphometric analysis of microscopic images by calculating the mean surface areas occupied by a positive immunohistochemical reaction in individual groups for all antibodies tested. Increased closure of substrates in the autophagosome (beclin 1) induced late endosome transport and accelerated autophagosome maturation process (RAB7). Furthermore, a larger number of autophagosomes (LC3B) was observed in liver cells immediately after the cessation of hyperbaric activity; however, this decreased after 5 days. During this time, chaperone-mediated autophagy (HSC73) was observed on a larger scale. This means that increased macroautophagy induced by hyperbaric treatment weakens with time that has elapsed since the cessation of high pressure, whereas similarly induced chaperone-mediated autophagy intensifies over time.
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MDPI and ACS Style
Pedrycz, A.; Kozakiewicz, M.; Rahnama, M.; Kos, M.; Grywalska, E.; Bracha, M.; Grzywacz, A.; Bojar, I.
Hyperbaric Treatment Stimulates Chaperone-Mediated Macroautophagy and Autophagy in the Liver Cells of Healthy Female Rats. Int. J. Mol. Sci. 2024, 25, 10476.
https://doi.org/10.3390/ijms251910476
AMA Style
Pedrycz A, Kozakiewicz M, Rahnama M, Kos M, Grywalska E, Bracha M, Grzywacz A, Bojar I.
Hyperbaric Treatment Stimulates Chaperone-Mediated Macroautophagy and Autophagy in the Liver Cells of Healthy Female Rats. International Journal of Molecular Sciences. 2024; 25(19):10476.
https://doi.org/10.3390/ijms251910476
Chicago/Turabian Style
Pedrycz, Agnieszka, Mariusz Kozakiewicz, Mansur Rahnama, Marek Kos, Ewelina Grywalska, Marietta Bracha, Anna Grzywacz, and Iwona Bojar.
2024. "Hyperbaric Treatment Stimulates Chaperone-Mediated Macroautophagy and Autophagy in the Liver Cells of Healthy Female Rats" International Journal of Molecular Sciences 25, no. 19: 10476.
https://doi.org/10.3390/ijms251910476
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