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Open AccessArticle
A Protocol for the Isolation of Oval Cells without Preconditioning
by
Rocío Olivera-Salazar
Rocío Olivera-Salazar 1,
Aránzazu Sánchez
Aránzazu Sánchez 2,3,
Blanca Herrera
Blanca Herrera 2,3,
Juan García-Sáez
Juan García-Sáez 2,3,
Luz Vega-Clemente
Luz Vega-Clemente 1,
Pedro Villarejo Campos
Pedro Villarejo Campos 4,
Damián García-Olmo
Damián García-Olmo 1,4,5 and
Mariano García-Arranz
Mariano García-Arranz 1,5,*
1
New Therapies Laboratory, Health Research Institute-Fundación Jiménez Díaz University Hospital (IIS-FJD), Avda. Reyes Católicos, 2, 28040 Madrid, Spain
2
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University of Madrid (UCM), Health Research Institute of the “Hospital Clínico San Carlos” (IdISSC), 28040 Madrid, Spain
3
Biomedical Research Networking Center in Hepatic and Digestive Diseases (CIBEREHD-ISCIII), 28029 Madrid, Spain
4
Department of Surgery, Fundación Jiménez Díaz University Hospital (FJD), 28040 Madrid, Spain
5
Department of Surgery, Universidad Autónoma de Madrid, 28034 Madrid, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(19), 10497; https://doi.org/10.3390/ijms251910497 (registering DOI)
Submission received: 30 August 2024
/
Revised: 20 September 2024
/
Accepted: 25 September 2024
/
Published: 29 September 2024
Abstract
Oval cells (OCs) is the name of hepatic progenitor cells (HPCs) in rodents. They are a small population of cells in the liver with the remarkable ability to proliferate and regenerate hepatocytes and cholangiocytes in response to acute liver damage. Isolating OCs generally requires a pretreatment with special diets, chemicals, and/or surgery to induce hepatic damage and OC proliferation in mice. Unfortunately, these pretreatments are not only painful for the mice but also increase the cost of the assays, and the effects on the different organs as well as on various liver cells are still unclear. Therefore, the search for a protocol to obtain OCs without prior liver damage is mandatory. In our study, we present a protocol to isolate murine OCs from healthy liver (HL-OCs) and compare them with OCs isolated from mice pretreated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC-OCs). Our results demonstrated that cells derived from untreated mice exhibited similar behavior to those from treated mice in terms of surface marker expression, proliferation, and differentiation capacity. Therefore, given the impracticability of isolating human cells with prior hepatotoxic treatment, our model holds promise for enabling the isolation of progenitor cells from human tissue in the future. This advancement could prove invaluable for translational medicine in the understanding and treatment of liver diseases.
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MDPI and ACS Style
Olivera-Salazar, R.; Sánchez, A.; Herrera, B.; García-Sáez, J.; Vega-Clemente, L.; Villarejo Campos, P.; García-Olmo, D.; García-Arranz, M.
A Protocol for the Isolation of Oval Cells without Preconditioning. Int. J. Mol. Sci. 2024, 25, 10497.
https://doi.org/10.3390/ijms251910497
AMA Style
Olivera-Salazar R, Sánchez A, Herrera B, García-Sáez J, Vega-Clemente L, Villarejo Campos P, García-Olmo D, García-Arranz M.
A Protocol for the Isolation of Oval Cells without Preconditioning. International Journal of Molecular Sciences. 2024; 25(19):10497.
https://doi.org/10.3390/ijms251910497
Chicago/Turabian Style
Olivera-Salazar, Rocío, Aránzazu Sánchez, Blanca Herrera, Juan García-Sáez, Luz Vega-Clemente, Pedro Villarejo Campos, Damián García-Olmo, and Mariano García-Arranz.
2024. "A Protocol for the Isolation of Oval Cells without Preconditioning" International Journal of Molecular Sciences 25, no. 19: 10497.
https://doi.org/10.3390/ijms251910497
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