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Article

Combined BSA-Seq and RNA-Seq Analysis to Identify Candidate Genes Associated with Aluminum Toxicity in Rapeseed (Brassica napus L.)

by
Huiwen Zhou
1,†,
Paolan Yu
2,†,
Lanhua Wu
1,
Depeng Han
2,
Yang Wu
1,
Wei Zheng
2,
Qinghong Zhou
3,* and
Xiaojun Xiao
2,*
1
Institute of Jiangxi Oil-Tea Camellia, College of Pharmacy and Life Science, Jiujiang University, Jiujiang 332005, China
2
Jiangxi Institute of Red Soil and Germplasm Resources, Key Laboratory of Arable Land Improvement and Quality Improvement of Jiangxi Province, Nanchang 330046, China
3
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Agronomy College, Jiangxi Agricultural University, Nanchang 330045, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Int. J. Mol. Sci. 2024, 25(20), 11190; https://doi.org/10.3390/ijms252011190
Submission received: 6 September 2024 / Revised: 7 October 2024 / Accepted: 12 October 2024 / Published: 17 October 2024
(This article belongs to the Section Molecular Genetics and Genomics)
Browse Figures
Versions Notes

Abstract

:
Exchangeable aluminum (Al) ions released from acidic soils with pH < 5.5 inhibit root elongation of crops, ultimately leading to yield reduced. It is necessary to identify the quantitative trait locus (QTLs) and candidate genes that confer toxicity resistance to understand the mechanism and improve tolerance of rapeseed. In this study, an F2 segregating population was derived from a cross between Al-tolerance inbred line FDH188 (R178) and -sensitive inbred line FDH152 (S169), and the F2:3 were used as materials to map QTLs associated with the relative elongation of taproot (RET) under Al toxicity stress. Based on bulked segregant analysis sequencing (BSA-seq), three QTLs (qAT-A07-1, qAT-A07-2, and qAT-A09-1) were detected as significantly associated with RET, and 656 candidate genes were screened. By combined BSA and RNA-seq analysis, 55 candidate genes showed differentially expressed, including genes encoding ABC transporter G (ABCG), zinc finger protein, NAC, ethylene-responsive transcription factor (ERF), etc. These genes were probably positive factors in coping with Al toxicity stress in rapeseed. This study provides new insight into exploring the QTLs and candidate genes’ response to Al toxicity stress by combined BSA-seq and RNA-seq and is helpful to further research on the mechanism of Al resistance in rapeseed.

1. Introduction

Aluminum (Al) is the most abundant metallic element found in the earth’s crust. When the soil pH value drops below 5.5, exchangeable Al ions (primarily A13+, Al(OH)2+, and Al(OH)2+) released from silicates or oxides can promote exponential [1,2]. Unfortunately, approximately 40% of the world’s potentially cultivable land exhibits a pH level below 5.5 [2,3,4]. Under Al toxicity stress, the first and most important symptom of crops is the inhibition of root elongation, ultimately affecting water and nutrient uptake [5,6,7,8]. The high-producing area of B. napus, one of the important oil crops, is the Yangtze River region in China. However, the soil in this area is mainly acidic soils (pH < 5.5), and the exchangeable Al ions were precipitated from the soil to form Al toxicity stress, which limits B. napus growth and yield [9,10,11]. Al toxicity has emerged as a significant factor impeding crop growth in these acidic soils.
In the long-term adaption of plants to Al toxicity, plants have indeed developed two main detoxification mechanisms: external exclusion and internal tolerance [12,13]. Exposed to Al toxicity stress, the root system of the plant was initially compromised, and the root cell wall was the primary line of defense by absorbing Al ions (about 30%~90% of Al ion absorbed) or secreting organic acids to chelate Al ions, thereby performing a crucial detoxification function [12,14]. It is the complex regulatory mechanism of plant response to Al toxicity, which is regulated by multiple genes and pathways. For instance, the ANAC017-XTH31 (xyloglucan endo-transglycosylases/hydrolase 31) model regulated the Al tolerance in Arabidopsis by adjusting the xiylogulcan content [15]. SbXTH7, acting downstream of SbHY5, regulated the Al tolerance of sweet sorghum by modulating cell wall hemicellulose content [16]. F-box proteins RAE1 (AtALMT1 expression 1) and RAH1 (RAE1 homolog) affect plant resistance to Al toxicity by regulating ubiquitination and the degradation of Al core transcription factor STOP1, while ALR1 (LRR receptor-like kinase) could reduce the degradation of STOP1 by increasing the content of reactive oxygen species (ROS) in plants and inhibit the interaction between RAE1 and STOP1, thus activating the secretion of organic acid anions for detoxification [17,18,19]. In B. napus, a few homologous genes were cloned and verified to improve the Al tolerance of transgenic plants [20,21,22]. The Al tolerance of plants is a complex trait, which requires further exploration of new genes.
In our previous study, 64 candidate genes showed differential expression at least in 6 h vs. 0 h or 24 h vs. 0 h of two lines by integration of genome-wide association analysis (GWAS) and RNA-seq analysis [23]. With the diversification of gene mapping methods, the efficacy of bulked segregant analysis sequencing (BSA-seq) in identifying trait-associated genes has been demonstrated through extensive application in various crops [24,25,26,27]. Based on combined BSA-seq and RNA-seq analysis, 32 candidate differentially expressed genes (DEGs) response to cadmium stress in B. napus were identified within nine significant mapping intervals, including genes encoding a glutathione S-transferase (GST), a molecular chaperone (DnaJ), and a phosphoglycerate kinase (PGK), among others [28]. Moreover, several candidate genes associated with nitrogen utilization efficiency (NUtE) in rapeseed were screened by integration of BSA-Seq and RNA-Seq analysis, such as the high-affinity nitrate transporter gene NRT2.1 (BnaC08g43370D) and the abscisic acid (ABA) signal transduction related genes (BnaC02g14540D, BnaA03g20760D, and BnaA05g01330D); overexpression of BnaA5.AIB could reduce the NUtE under low N levels in Arabidopsis [25].
In this study, a population of F2 generation was constructed by crossing the Al-tolerant inbred line FDH188 (R178) and the Al-sensitive inbred line FDH152 (S169). The representative type F2:3 Al-tolerance was identified to select the extreme population, and then BSA-seq was employed to re-sequence the whole genome of parents and two extreme phenotypic progeny mixing pools. SNP markers were utilized for locating the target trait association region. Then, the differentially expressed genes associated with Al toxicity stress were identified in QTLs by combination with our previous transcriptome sequencing. The aim of this study was to explore Al-resistant genes in rapeseed and to provide new insights into the molecular mechanism of Al-resistant rapeseed.

2. Result

2.1. Statistical Analysis of the Phenotype of the F2:3 Generation Population

After seven days of Al toxicity stress, the RET of R178 and S169 were 0.868 and 0.413, respectively (Figure 1). The phenotypic data of the F2:3 population showed that the F2:3 population exhibited maximum, minimum, and average relative elongation of taproot of 0.930, 0.281, and 0.600, and the kurtosis was −0.304; Shapiro–Wilk and Kolmogorov Smirnov tests conform to normal distribution (Figure 2 and Table 1). The results indicated that RET in the F2:3 population exhibits an unimodal continuous distribution without an obvious proportional relationship, exhibiting the genetic characteristics as a quantitative trait. Therefore, it is subject to polygenic regulation and suitable for QTL analysis using BSA-seq.

2.2. Assessment of Sequencing Quality, SNP Detection, and Annotation

A total of 1.0 billion high-quality clean reads with GC content ranging from 37.91% to 38.56% were generated (Supplementary Table S1). After the clean reads mapped to reference genome Brassica napus V.1, the results showed that the average sequencing depth was higher than 29 (Supplementary Table S2).
The SNP analysis results showed that 2,978,096, 3,297,428, 4,220,595, and 4,127,362 SNPs were detected in the R178, S169, ATL pool, and ASL pool, respectively. The proportion of SNP within the intergenic was found to be the highest, accounting for no less than 70%, while the number of SNP associated with splice site donors was observed to be the lowest (Supplementary Table S3). With the utilization of SNP markers Δ(SNP-index), regression fitting is performed. After fitting, when the confidence level of the delta SNP-index reaches 99% confidence interval, four QTLs are located outside the confidence interval, including A07 (15,402,793~18,799,754, 20,308,564~20,760,352), A09 (26,382,178~27,159,317), and C08 (6,061,199~6,099,270) (Figure 3, Supplementary Table S4).

2.3. Indel Detection and Annotation

The Indel analysis results showed that 1,004,146, 1,025,346, 1,060,849, and 1,054,800 Indels were detected in the R178, S169, ATL pool, and ASL pool, respectively. The proportion of Indel mutation sites within the intergenic was found to be the highest, accounting for no less than 65%, while the number of Indel mutation sites associated with exon deletions and stop losses was observed to be the lowest (Supplementary Table S5).
With the utilization of SNP markers Δ(Indel-index), regression fitting is performed. After fitting, when the confidence level of the delta Indel-index reaches 99% confidence interval, four QTLs are located outside the confidence interval, including A02 (9,219,187~9,219,189), A07 (15,712,254~19,360,892, 2,068,093~20,755,053), and A09 (26,393,183~27,036,238) (Figure 4, Supplementary Table S6).

2.4. QTL for Al-Tolerance Identified by SNP and Indel Markers

Based on the integration of SNP and Indel markers, three QTLs on two chromosomes (A07 and A09) were identified with the confidence of Δ(SNP-index) and Δ(Indel-index) > 99%, containing qAT-A07-1 (15,712,254~18,799,754), qAT-A07-2 (20,680,931~20,755,053), and qAT-A09-2 (3,125,541~3,153,126) (Table 2). Among these QTLs, qAT-A07-1 is the longest. A total of 656 candidate genes distributed in three QTLs were located outside the 99% confidence interval, 554 candidate genes were distributed in two QTLs on chr.A07, 542 candidate genes in qAT-A07-1, and 102 candidate genes were distributed in one QTL on chr.A09 (Table 2, Supplementary Table S7).

2.5. Candidate Differentially Expresssed Genes (DEGs) Analysis

For RNA-seq, compared with 0 h, there were 2618 DEGs both at 6 h in R178 and 6 h/24 h in S169, and 576 DEGs both at 24 h in R178 and 6 h/24 h in S169 [23]. In addition, there were 8279 DEGs in R178 6 h vs. S169 6 h (4484 up-regulated and 3795 down-regulated) and 8776 in R178 24 h vs. S169 24 h (4330 up-regulated and 4446 down-regulated), including 4481 DEGs both in R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h (2108 DEGs both up-regulated, 2366 DEGs both down-regulated, four DEGs up-regulated at 6 h and down-regulated at 24 h, and three DEGs down-regulated at 6 h and up-regulated at 24 h) (Supplementary Figure S1). The combined analysis of BSA-seq and our previous RNA-seq showed that 55 candidate genes were differentially expressed, including 11 candidate DEGs both at 6 h in R178 and 6 h/24 h in S169, six candidate DEGs both at 24 h in R178 and 6 h/24 h in S169, and 45 candidate DEGs both in R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h (Table 3).
There were 46 candidate DEGs in qAT-A07-1, two candidate DEGs in qAT-A07-2, and seven candidate DEGs in qAT-A09-1 (Table 3). Among these candidate genes, 10 genes showed differential expressions at 6 h vs. 0 h both in R178 and S169 and two genes at 24 h vs. 0 h both in R178 and S169. At 6 h vs. 0 h, six candidate genes were up-regulated both in R178 and S169, with four candidate genes both being down-regulated. At 24 h vs. 0 h, one candidate gene was up-regulated both in R178 and S169 and one gene was both down-regulated. Among R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h, 21 candidate genes were both up-regulated, 23 candidate genes were both down-regulated, and one gene was down-regulated in R178 6 h vs. S169 6 h and up-regulated in R178 24 h vs. S169 24 h. Following gene functional annotation and classification of the 55 candidate DEGs, 48 DEGs were found to be of known function, including genes encoding ABC transporter G (ABCG), zinc finger protein, NAC and ethylene-responsive transcription factor (ERF), etc., and seven DEGs uncharacterized protein.

3. Discussion

Al toxicity inhibited root elongation of crop and significantly decreased the relative elongation of taproot (RET) [12,16,23], which suggests that RET, as one of the main traits, was used for QTLs under Al toxicity stress [29,30]. There were 13 new candidate regions related with the relative root elongation and 69 new candidate genes response to Al toxicity in candidate regions detected in rice [30]; two QTL (qAl_Gm13 and qAl_Gm20) were significantly associated with primary root length ration and may have helped maintain root elongation and initiation in soybean [29]. In our previous study, 43 SNPs significantly associated with eight Al-tolerance traits in the seedling stage were detected by GWAS, and 64 candidate genes from the flanking 100 kb region of these SNPs were screened by integration of GWAS and RNA-seq analysis [23]. During germination of rapeseed, eight SNPs (located on chromosomes A03, A07, A09, A10, C05, C06, and C09) and five SNPs (located on chromosomes A03, A04, A10, C05, and C07) were detected as significantly associated with relative root length and relative dry weight under Al toxicity [31]. In addition, 23 QTLs for five traits in rape germination period detected, including 4 QTLs related with relative root length [32], and 44 DEGs were screened by integrating the results of RNA-seq and QTL mapping of root-related traits at germination stage under Al toxicity [33]. In this study, RET of R178 (0.868) were higher than S169 (0.413) under Al toxicity stress (Figure 1). Based on the integration of SNP and Indel markers, three QTLs on two chromosomes (A07 and A09) with RET were identified between ATL and ASL, including qAT-A07-1, qAT-A07-2, and qAT-A09-1 (Table 2). Compared with previous studies [23,31,32], three QTLs detected in current research were the novel discovered loci. And among these QTLs, a total of 55 candidate DEGs were detected by combining BSA-seq with RNA-seq, including 48 DEGs with known function and seven uncharacterized function.
In the complex process of detoxifying Al toxicity, there are many transcription factors involved in regulating the response of crops to Al stress [34,35,36,37]. The zinc finger protein STOP1 regulated plant response to Al toxicity by increasing the expression of MATE and ALMT, resulting in increasing the organic acid transported for detoxification [38,39]. OsWRKY22 enhances the expression level of OsFRDL4, thus improving Al-induced citrate secretion and Al tolerance in rice [40]. Both CML24 and CAMTA2 interacted with WRKY46 and alleviated the transcriptional repression of ALMT1 by WRKY46, which increased malate secretion and enhanced the Al-tolerance of the plant [36]. NAC transcription factors VnNAR1 and ANAC017 regulated Al tolerance by regulating the cell wall metabolism [41,42]. Moreover, over-expression of GmABR1 (ERF transcription factor) and GsERF1 significantly increased Al resistance in Arabidopsis and decreased the content of Al ions in the root tips [43,44]. In this study, seven transcription factors in qAT-A07-1 were detected by combining BSA-seq with RNA-seq (Table 3). Two candidate DEGs (BnaA07g20030D and BnaA07g21640D) related to zinc finger proteins showed down-regulation in R178 (6 h vs. 0 h and 24 h vs. 0 h), R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h, respectively. NAC transcription factor 29 (BnaA07g24270D) showed down-regulation in S169 (6 h vs. 0 h and 24 h vs. 0 h), R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h. The probable WRKY transcription factor 57 (BnaA07g24310D) showed down-regulation in R178 (6 h vs. 0 h and 24 h vs. 0 h) and S169 (6 h vs. 0 h). The results showed that these candidate transcription factors play an important regulatory role in B. napus response to Al toxicity, but the regulatory mechanism needs to be further verified.
Under Al toxicity stress, many membrane transporters play vital regulatory roles in the process of plant detoxification. For instance, genes related to the encoding Al-activated malate transporter (SgALMT2, BnALMT1, etc.) and multidrug and toxic compound extrusion (GmMATE, ZmMATE1, etc.) increased organic acid secretion in the roots to chelate Al ions, which participate in the detoxification process [21,45,46,47]. Moreover, ATP-binding cassette (ABC) transporter proteins, which are also involved in the transport and response of abacisic acid (ABA) and regulate the activity of glucosyltransferase/hydrolase in xyloglucan to affect the cell wall, play an important role in the growth and response to abiotic stress [48,49,50]. OsSTAR1 (for sensitive to Al rhizotoxicity 1) and OsSTAR2 as an ABC transporter may be used to modify the cell wall by transporting UDP-glucose for detoxification of Al in rice [50]. Knockout of OsABCG36 resulted in increased cadmium accumulation in root cell sap and enhanced cadmium sensitivity [51]. In this study, one ABC transporter G family member 25 (BnaA07g23320D) in qAT-A07-1 was detected by combining BSA-seq with RNA-seq. The expression of BnaA07g23320D was both down-regulated in R178 and S169 under 6 h vs. 0 h and down-regulated in R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h. AtABCG25 is an exporter of ABA and is involved in the intercellular ABA signaling response to environmental stress among plant cells [49]. In addition, the exogenous application of ABA could increase the activity of citrate synthase and decrease Al accumulation [52,53]. In addition to ABA, Al3+-induced ethylene production is likely to act as a signal to alter auxin distribution in roots, which leads to local auxin accumulation in the root–apex transition zone and the arrest of root elongation [54,55,56]. Phosphoethanolamine N-methyltransferase 1 (PEAMT1) was an important regulator of root development by affecting ROS over-accumulation or inhibition of auxin signaling under choline starvation [57]. In this study, one candidate DEG (BnaA07g23650D) encoding the ethylene-responsive transcription factor was screened and showed up-regulation in R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h. One candidate DEG (BnaA07g22620D) encoding PEAMT was up-regulated at 6 h in R178 and 24 h in S169 compared with 0 h and was up-regulated in R178 6 h vs. S169 6 h. These results showed that the Al toxicity stress-induced DEGs related to the ABC transporter and ethylene-responsive transcription factor, which may affect auxin accumulation in the root–apex and inhibit root elongation.

4. Materials and Methods

4.1. Plant Materials

The Al-tolerant inbred line (FDH188, R178) and the Al-sensitive inbred line (FDH152, S169) were obtained from the Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding of the Ministry of Education at Jiangxi Agricultural University [23]. R178 was selected as the male parent and S169 as the female parent; 274 F2 individual populations were used in this study.

4.2. Phenotypic Identification of Parents and F2:3 Population

Seeds of R178, S169, and F2:3 populations with full and uniform were sown on gauze in a square plastic bowl filled with pure water. After six days, the uniform and healthy seedlings were sequentially transferred into 1/4 and 1/2 Hoagland’s nutrient solution (Coolaibo Technology, Beijing, China), and each nutrient solution was cultured for three days under 25 °C-14 h/20 °C-10 h (day/night). Subsequently, the seedlings were transplanted into 0.5 mmol·L−1 CaCl2 solution (pH4.5) for 24 h. Finally, the seedlings were exposed to the 1/2 nutrient solution containing 150 µmol·L−1 AlCl3 and 0.5 mmol·L−1 CaCl2 for seven days of Al toxicity stress, with 0 µmol·L−1 AlCl3 as the control. The length of the taproot was measured both at the beginning of treatment and seven days after treatment. The relative elongation of taproot (RET) was calculated: the relative elongation of taproot = (treatment root elongation/control root elongation). The resulting data were analyzed using Excel and Origin to identify outliers within the F2 generation population. Three biological replicates were performed.

4.3. Construction of DNA Mixed Pool and Re-Sequencing

The genomic DNA of parental materials (R178, S169) and extreme individuals of the F2 generation population were extracted from young leaves by the CTAB method. Then, the pool of Al tolerance lines (ATL pool) was constructed by equally mixing the DNA from 27 Al-tolerance individuals. A pool of Al-sensitive lines (ASL pool) was constructed by equally mixing the DNA from 27 Al-sensitive individuals. After qualifying the DNA of R178, S169, ATL pool, and ASL pool, whole-genome re-sequencing was performed using the Illumina HiSeq platform (Genepioneer Biotechnology Co., Ltd., Nanjing, China). Then, the quality of the original reads was assessed, and the cleaned reads from four pools were compared to the reference genome sequence of Brassica napus V.1 by BWA software (0.7.17-r1188) [58]. Based on GATK (3.7-0-gcfedb67) [59] and SnpEff (v4_5covid19) [60], the SNPs and Indels were identified and annotated based on the comparison results.

4.4. Correlation Region Analysis of BSA

The Euclidean distance method (ED) is utilized to calculate the Euclidean distances between mixtures, with greater distances indicating larger differences. Regression fitting is performed on SNP marker Δ(SNP-index) and Indel marker Δ(Indel-index) located on the same chromosome to obtain a correlation threshold. The region above the 99% confidence interval of this threshold is selected as the correlation region related to the target trait.

4.5. Combined Analysis of BSA-Seq and RNA-Seq

For RNA-seq, R178 and S169 were treated with 150 µmol·L−1 AlCl3 for 0 h (control), 6 h, and 24 h, respectively, which has been described in our previous research [23]. To detect the DEGs, compared with 0 h, the genes with a false discovery rate ≤ 0.05 and |log2(fold change)| ≥ 1.0 were determined as the significantly differential expression under 6 h or 24 h Al toxicity stress in R178 and S169. In addition, the genes with a false discovery rate ≤ 0.05 and |log2(fold change)| ≥ 1.0 of 6 h in R178 vs. S169 or 24 h in R178 vs. S169 were considered the significantly differentially expressed genes.
After analyzing overlapping regions of SNP marker Δ(SNP-index) and Indel marker Δ(Indel-index), the candidate genes in overlapping regions were obtained. Then, combined with the results of previous RNA-seq under Al toxicity stress [23], the candidate genes with differential expression were screened. The function of candidate DEG was annotated using NR (ftp://ftp.ncbi.nih.gov/blast/db/FASTA/ (accessed on 25 March 2023)), SwissProt (http://www.expasy.org/sprot/ (accessed on 26 March 2023)).

5. Conclusions

In summary, three QTLs (qAT-A07-1, qAT-A07-2, and qAT-A09-1) were identified with the confidence of Δ(SNP-index) and Δ(Indel-index) > 99%, and 656 candidate genes were screened among these QTLs. By combining the analysis of BSA-seq and our previous RNA-seq, 55 candidate genes showed differential expression, including 11 candidate DEGs both at 6 h in R178 and 6 h/24 h in S169, 6 candidate DEGs both at 24 h in R178 and 6 h/24 h in S169, and 45 candidate DEGs both in R178 6 h vs. S169 6 h and R178 24 h vs. S169 24 h. In total, 48 out of 55 candidate DEGs with known function were detected, including genes encoding ABC transporter G (ABCG), zinc finger protein, NAC, ERF, etc. These candidate DEGs may play an important regulatory role in the B. napus response to Al toxicity. The combined analysis of BSA-seq and RNA-seq provided an effective strategy to explore the QTLs and candidate genes, which was useful in understanding the molecular mechanisms response to Al toxicity stress in B. napus.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/ijms252011190/s1.

Author Contributions

X.X. and Q.Z. designed the research plan; H.Z., P.Y. and L.W. performed the research work; D.H., Y.W. and W.Z. analyzed the data; H.Z., P.Y. and X.X. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the National Natural Science Foundation of China project (32260458), the Jiangxi Provincial Natural Science Foundation (20224BAB205021), the Key Laboratory of Arable Land Improvement and Quality Improvement of Jiangxi Province (2024SSY04223), and the Jiangxi Training Project of high-level and high-skill leading talents (2023).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in this article and Supplementary Materials.

Acknowledgments

We thank Genepioneer Biotechnology (Nanjing, China) for BSA-seq in this study.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Shetty, R.; Vidya, C.S.; Prakash, N.B.; Lux, A.; Vaculík, M. Aluminum toxicity in plants and its possible mitigation in acid soils by biochar: A review. Sci. Total Environ. 2021, 765, 142744. [Google Scholar] [CrossRef] [PubMed]
  2. Kochian, L.V.; Piñeros, M.A.; Liu, J.; Magalhaes, J.V. Plant Adaptation to Acid Soils: The Molecular Basis for Crop Aluminum Resistance. Annu. Rev. Plant Biol. 2015, 66, 571–598. [Google Scholar] [CrossRef] [PubMed]
  3. von Uexküll, H.; Mutert, E. Global extent, development and economic impact of acid soils. Plant Soil 1995, 171, 1–15. [Google Scholar] [CrossRef]
  4. Zhu, X.F.; Shen, R.F. Towards sustainable use of acidic soils: Deciphering aluminum-resistant mechanisms in plants. Fundam. Res. 2023, 14, 41. [Google Scholar] [CrossRef]
  5. Mejia-Alvarado, F.S.; Botero-Rozo, D.; Araque, L.; Bayona, C.; Herrera-Corzo, M.; Montoya, C.; Ayala-Díaz, I.; Romero, H.M. Molecular network of the oil palm root response to aluminum stress. BMC Plant Biol. 2023, 23, 346. [Google Scholar] [CrossRef]
  6. Liu, H.; Zhu, R.; Shu, K.; Lv, W.; Wang, S.; Wang, C. Aluminum stress signaling, response, and adaptive mechanisms in plants. Plant Signal. Behav. Plant Signal. Behav. 2022, 17, 2057060. [Google Scholar] [CrossRef]
  7. Tohidi, Z.; Baghizadeh, A.; Enteshari, S. The Effects of Aluminum and Phosphorous on Brassica napus. Am. Eurasian J. Agric. Environ. Sci. 2009, 6, 137–142. [Google Scholar]
  8. Roshani, M.; Abbaspour, H.; Saeidi-sar, S. Effect of aluminium stress on germination and mineral nutrition of kidney bean cultivars with diferent sensitivity to aluminium. Biosci. Biotechnol. Res. Asia 2014, 11, 545–553. [Google Scholar] [CrossRef]
  9. Guo, J.H.; Liu, X.J.; Zhang, Y.; Shen, J.L.; Han, W.X.; Zhang, W.F.; Christie, P.; Goulding, K.W.; Vitousek, P.M.; Zhang, F.S. Significant acidification in major Chinese croplands. Science 2010, 327, 1008–1010. [Google Scholar] [CrossRef]
  10. Ryan, P.R.; Tyerman, S.D.; Sasaki, T.; Furuichi, T.; Yamamoto, Y.; Zhang, W.H.; Delhaize, E. The identification of aluminium-resistance genes provides opportunities for enhancing crop production on acid soils. J. Exp. Bot. 2011, 62, 9–20. [Google Scholar] [CrossRef]
  11. Wu, J.; Guo, X.S.; Wang, W.J.; Zhu, H.B. Effect of dolomite application on soil acidity and yield of rapeseed on yellow-red soil. Chin. J. Oil Crop Sci. 2006, 28, 55–58. [Google Scholar]
  12. Rahman, R.; Upadhyaya, H. Aluminium Toxicity and Its Tolerance in Plant: A Review. J. Plant Biol. Singmul Hakhoe Chi 2021, 64, 101–121. [Google Scholar] [CrossRef]
  13. Yang, J.L.; Fan, W.; Zheng, S.J. Mechanisms and regulation of aluminum-induced secretion of organic acid anions from plant roots. J. Zhejiang Univ. Sci. B 2019, 20, 513–527. [Google Scholar] [CrossRef] [PubMed]
  14. Shweta, S.; Durgesh, K.T.; Swati, S.; Shivesh, S.; Nawal Kishore, D.; Devendra, K.C.; Marek, V. Toxicity of aluminium on various levels of plant cells and organism: A review. Environ. Exp. Bot. 2017, 137, 177–193. [Google Scholar] [CrossRef]
  15. Li, S.; Yang, J.B.; Li, J.Q.; Huang, J.; Shen, R.F.; Zeng, D.L.; Zhu, X.F. A NAC transcription factor represses a module associated with xyloglucan content and regulates aluminum tolerance. Plant Physiol. 2024, 196, 564–578. [Google Scholar] [CrossRef] [PubMed]
  16. Zhan, M.; Li, H.; You, J.; Meng, X.; Guan, K.; Zheng, M.; Gao, J.; Yang, Z. SbXTH7, acting downstream of transcription factor SbHY5, regulates aluminum tolerance by modulating cell wall hemicellulose content and aluminum accumulation in sweet sorghum [Sorghum bicolor (L.)]. Ind. Crop. Prod. 2024, 214, 118485. [Google Scholar] [CrossRef]
  17. Ding, Z.J.; Xu, C.; Yan, J.Y.; Wang, Y.X.; Cui, M.Q.; Yuan, J.J.; Wang, Y.N.; Li, G.X.; Wu, J.X.; Wu, Y.R.; et al. The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor. Cell Res. 2024, 34, 281. [Google Scholar] [CrossRef]
  18. Fang, Q.; Zhou, F.; Zhang, Y.; Singh, S.; Huang, C.F. Degradation of STOP1 mediated by the F-box proteins RAH1 and RAE1 balances aluminum resistance and plant growth in Arabidopsis thaliana. Plant J. 2021, 106, 493–506. [Google Scholar] [CrossRef]
  19. Zhang, Y.; Zhang, J.; Guo, J.; Zhou, F.; Singh, S.; Xu, X.; Xie, Q.; Yang, Z.; Huang, C. F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis. Proc. Natl. Acad. Sci. USA 2019, 116, 319–327. [Google Scholar] [CrossRef]
  20. Wang, Y.; Xu, H.; Kou, J.; Shi, L.; Zhang, C.; Xu, F. Dual effects of transgenic Brassica napus overexpressing CS gene on tolerances to aluminum toxicity and phosphorus deficiency. Plant Soil 2013, 362, 231–246. [Google Scholar] [CrossRef]
  21. Ligaba, A.; Katsuhara, M.; Ryan, P.R.; Shibasaka, M.; Matsumoto, H. The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells. Plant Physiol. 2006, 142, 1294–1303. [Google Scholar] [CrossRef] [PubMed]
  22. Basu, U.; Good, A.G.; Taylor, G.J. Transgenic Brassica napus plants overexpressing aluminium—Induced mitochondrial manganese superoxide dismutase cDNA are resistant to aluminium. Plant Cell Environ. 2001, 24, 1269–1278. [Google Scholar] [CrossRef]
  23. Zhou, H.; Xiao, X.; Asjad, A.; Han, D.; Zheng, W.; Xiao, G.; Huang, Y.; Zhou, Q. Integration of GWAS and transcriptome analyses to identify SNPs and candidate genes for aluminum tolerance in rapeseed (Brassica napus L.). BMC Plant Biol. 2022, 22, 130. [Google Scholar] [CrossRef] [PubMed]
  24. Lei, S.; Chen, L.; Liang, F.; Zhang, Y.; Zhang, C.; Xiao, H.; Tang, R.; Yang, B.; Wang, L.; Jiang, H. Identification of a major QTL and candidate genes analysis for branch angle in rapeseed (Brassica napus L.) using QTL-seq and RNA-seq. Front. Plant Sci. 2024, 15, 1340892. [Google Scholar] [CrossRef] [PubMed]
  25. Nan, Y.; Xie, Y.; He, H.; Wu, H.; Gao, L.; Atif, A.; Zhang, Y.; Tian, H.; Hui, J.; Gao, Y. Integrated BSA-seq and RNA-seq analysis to identify candidate genes associated with nitrogen utilization efficiency (NUtE) in rapeseed (Brassica napus L.). Int. J. Biol. Macromol. 2024, 254, 127771. [Google Scholar] [CrossRef]
  26. Zheng, R.; Deng, M.; Lv, D.; Tong, B.; Liu, Y.; Luo, H. Combined BSA-Seq and RNA-Seq Reveal Genes Associated with the Visual Stay-Green of Maize (Zea mays L.). Int. J. Mol. Sci. 2023, 24, 17617. [Google Scholar] [CrossRef]
  27. Majeed, A.; Johar, P.; Raina, A.; Salgotra, R.K.; Feng, X.; Bhat, J.A. Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding. Front. Genet. 2022, 13, 944501. [Google Scholar] [CrossRef]
  28. Wang, H.; Liu, J.; Huang, J.; Xiao, Q.; Hayward, A.; Li, F.; Gong, Y.; Liu, Q.; Ma, M.; Fu, D.; et al. Mapping and identifying candidate genes enabling cadmium accumulation in brassica napus revealed by combined BSA-Seq and RNA-Seq Analysis. Int. J. Mol. Sci. 2023, 24, 10163. [Google Scholar] [CrossRef]
  29. Li, Y.; Ye, H.; Song, L.; Vuong, T.D.; Song, Q.; Zhao, L.; Shannon, J.G.; Li, Y.; Nguyen, H.T. Identification and characterization of novel QTL conferring internal detoxification of aluminium in soybean. J. Exp. Bot. 2021, 72, 4993–5009. [Google Scholar] [CrossRef]
  30. Zhang, P.; Zhong, K.; Zhong, Z.; Tong, H. Mining candidate gene for rice aluminum tolerance through genome wide association study and transcriptomic analysis. BMC Plant Biol. 2019, 19, 490. [Google Scholar] [CrossRef]
  31. Gao, H.; Ye, S.; Wu, J.; Wang, L.; Wang, R.; Lei, W.; Meng, L.; Yuan, F.; Zhou, Q.; Cui, C. Genome-wide association analysis of aluminum tolerance related traits in rapeseed (Brassica napus L.) during germination. Genet. Resour. Crop Evol. 2021, 68, 335–357. [Google Scholar] [CrossRef]
  32. Wang, R.L.; Wang, L.Y.; Ye, S.; Gao, H.H.; Lei, W.; Wu, J.Y.; Yuan, F.; Meng, L.J.; Tang, Z.L.; Li, J.N.; et al. QTL mapping of seed germination-related traits in Brassica napus L. under aluminum toxicity stress. Acta Agron. Sin. 2020, 46, 832–843. [Google Scholar]
  33. Wang, R.L.; Wang, L.Y.; Lei, W.; Wu, J.Y.; Shi, H.S.; Li, C.Y.; Tang, Z.L.; Li, J.N.; Zhou, Q.Y.; Cui, C. Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L. Acta Agron. Sin. 2021, 47, 2407–2422. [Google Scholar]
  34. Su, N.; Gong, Y.; Hou, X.; Liu, X.; Shabala, S.; Demidchik, V.; Yu, M.; Jiang, M.; Huang, L. Zinc finger protein ZFP36 and pyruvate dehydrogenase kinase PDK1 function in ABA-mediated aluminum tolerance in rice. Crop J. 2024. [Google Scholar] [CrossRef]
  35. Liu, L.; Cheng, L.; Liu, K.; Yu, T.; Liu, Q.; Gong, Z.; Cai, Z.; Liu, J.; Zhao, X.; Nian, H.; et al. Transgenic soybean of GsMYB10 shapes rhizosphere microbes to promote resistance to aluminum (Al) toxicity. J. Hazard. Mater. 2023, 455, 131621. [Google Scholar] [CrossRef]
  36. Zhu, X.; Wang, P.; Bai, Z.; Herde, M.; Ma, Y.; Li, N.; Liu, S.; Huang, C.F.; Cui, R.; Ma, H.; et al. Calmodulin—Like protein CML24 interacts with CAMTA2 and WRKY46 to regulate ALMT1—Dependent Al resistance in Arabidopsis thaliana. New Phytol. 2022, 233, 2471–2487. [Google Scholar] [CrossRef]
  37. Zhang, L.; Dong, D.; Wang, J.; Wang, Z.; Zhang, J.; Bai, R.Y.; Wang, X.; Rubio, W.M.; Blumwald, E.; Zhang, N.; et al. A zinc finger protein SlSZP1 protects SlSTOP1 from SlRAE1-mediated degradation to modulate aluminum resistance. New Phytol. 2022, 236, 165–181. [Google Scholar] [CrossRef]
  38. Huang, C. Activation and activity of STOP1 in aluminium resistance. J. Exp. Bot. 2021, 72, 2269–2272. [Google Scholar] [CrossRef]
  39. Tokizawa, M.; Enomoto, T.; Ito, H.; Wu, L.; Kobayashi, Y.; Mora-Macías, J.; Armenta-Medina, D.; Iuchi, S.; Kobayashi, M.; Nomoto, M.; et al. High affinity promoter binding of STOP1 is essential for the early aluminum-inducible expression of novel Al resistance genes GDH1 and GDH2 in Arabidopsis. J. Exp. Bot. 2021, 72, 2769–2789. [Google Scholar] [CrossRef]
  40. Li, G.Z.; Wang, Z.Q.; Yokosho, K.; Ding, B.; Fan, W.; Gong, Q.Q.; Li, G.X.; Wu, Y.R.; Yang, J.L.; Ma, J.F.; et al. Transcription factor WRKY22 promotes aluminum tolerance via activation of OsFRDL4 expression and enhancement of citrate secretion in rice (Oryza sativa). New Phytol. 2018, 219, 149–162. [Google Scholar] [CrossRef]
  41. Tao, Y.; Wan, J.X.; Liu, Y.S.; Yang, X.Z.; Shen, R.F.; Zhu, X.F. The NAC transcription factor ANAC017 regulates aluminum tolerance by regulating the cell wall-modifying genes. Plant Physiol. 2022, 189, 2517–2534. [Google Scholar] [CrossRef] [PubMed]
  42. Lou, H.Q.; Fan, W.; Jin, J.F.; Xu, J.M.; Chen, W.W.; Yang, J.L.; Zheng, S.J. A NAC-type transcription factor confers aluminum resistance by regulating cell wall-associated receptor kinase 1 and cell wall pectin. Plant Cell Environ. 2020, 43, 463–478. [Google Scholar] [CrossRef] [PubMed]
  43. Wang, H.; Li, C.; Wang, L.; Zhong, H.; Xu, X.; Cheng, Y.; Nian, H.; Liu, W.; Chen, P.; Zhang, A.; et al. GmABR1 encoding an ERF transcription factor enhances the tolerance to aluminum stress in Arabidopsis thaliana. Front. Plant Sci. 2023, 14, 1125245. [Google Scholar] [CrossRef] [PubMed]
  44. Li, L.; Li, X.; Yang, C.; Cheng, Y.; Cai, Z.; Nian, H.; Ma, Q. GsERF1 enhances Arabidopsis thaliana aluminum tolerance through an ethylene-mediated pathway. BMC Plant Biol. 2022, 22, 258. [Google Scholar] [CrossRef]
  45. Zhou, Y.; Wang, Z.; Gong, L.; Chen, A.; Liu, N.; Li, S.; Sun, H.; Yang, Z.; You, J. Functional characterization of three MATE genes in relation to aluminum-induced citrate efflux from soybean root. Plant Soil 2019, 443, 121–138. [Google Scholar] [CrossRef]
  46. Maron, L.G.; Pineros, M.A.; Guimaraes, C.T.; Magalhaes, J.V.; Pleiman, J.K.; Mao, C.; Shaff, J.; Belicuas, S.N.; Kochian, L.V. Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize. Plant J. 2010, 61, 728–740. [Google Scholar] [CrossRef]
  47. Miao, Y.; Hu, X.; Wang, L.; Schultze-Kraft, R.; Wang, W.; Chen, Z. Characterization of SgALMT genes reveals the function of SgALMT2 in conferring aluminum tolerance in Stylosanthes guianensis through the mediation of malate exudation. Plant Physiol. Biochem. 2024, 208, 108535. [Google Scholar] [CrossRef]
  48. Huang, J.; Li, X.; Chen, X.; Guo, Y.; Liang, W.; Wang, H. Genome-Wide Identification of Soybean ABC Transporters Relate to Aluminum Toxicity. Int. J. Mol. Sci. 2021, 22, 6556. [Google Scholar] [CrossRef]
  49. Kuromori, T.; Miyaji, T.; Yabuuchi, H.; Shimizu, H.; Sugimoto, E.; Kamiya, A.; Moriyama, Y.; Shinozaki, K. ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proc. Natl. Acad. Sci. USA 2010, 107, 2361–2366. [Google Scholar] [CrossRef]
  50. Huang, C.F.; Yamaji, N.; Mitani, N.; Yano, M.; Nagamura, Y.; Ma, J.F. A bacterial-type ABC transporter is involved in aluminum tolerance in rice. Plant Cell 2009, 21, 655–667. [Google Scholar] [CrossRef]
  51. Fu, S.; Lu, Y.; Zhang, X.; Yang, G.; Chao, D.; Wang, Z.; Shi, M.; Chen, J.; Chao, D.; Li, R.; et al. The ABC transporter ABCG36 is required for cadmium tolerance in rice. J. Exp. Bot. 2019, 70, 5909–5918. [Google Scholar] [CrossRef] [PubMed]
  52. Fan, W.; Xu, J.M.; Wu, P.; Yang, Z.X.; Lou, H.Q.; Chen, W.W.; Jin, J.F.; Zheng, S.J.; Yang, J.L. Alleviation by abscisic acid of Al toxicity in rice bean is not associated with citrate efflux but depends on ABI5-mediated signal transduction pathways. J. Integr. Plant Biol. 2019, 61, 140–154. [Google Scholar] [CrossRef]
  53. Hou, N.; You, J.; Pang, J.; Xu, M.; Chen, G.; Yang, Z. The accumulation and transport of abscisic acid insoybean (Glycine max L.) under aluminum stress. Plant Soil 2010, 330, 127–137. [Google Scholar] [CrossRef]
  54. Li, C.; Liu, G.; Geng, X.; He, C.; Quan, T.; Hayashi, K.I.; De Smet, I.; Robert, H.S.; Ding, Z.; Yang, Z.B. Local regulation of auxin transport in root—Apex transition zone mediates aluminium—Induced Arabidopsis root—Growth inhibition. Plant J. 2021, 108, 55–66. [Google Scholar] [CrossRef] [PubMed]
  55. Yang, Z.; Geng, X.; He, C.; Zhang, F.; Wang, R.; Horst, W.J.; Ding, Z. TAA1-regulated local auxin biosynthesis in the root-apex transition zone mediates the aluminum-induced inhibition of root growth in Arabidopsis. Plant Cell 2014, 26, 2889–2904. [Google Scholar] [CrossRef] [PubMed]
  56. Sun, P.; Tian, Q.Y.; Chen, J.; Zhang, W.H. Aluminium-induced inhibition of root elongation in Arabidopsis is mediated by ethylene and auxin. J. Exp. Bot. 2010, 61, 347–356. [Google Scholar] [CrossRef]
  57. Zou, Y.; Zhang, X.; Tan, Y.; Huang, J.B.; Zheng, Z.; Tao, L.Z. Phosphoethanolamine N-methyltransferase 1 contributes to maintenance of root apical meristem by affecting ROS and auxin-regulated cell differentiation in Arabidopsis. New Phytol. 2019, 224, 258–273. [Google Scholar] [CrossRef]
  58. Li, H.; Durbin, R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 2009, 25, 1754–1760. [Google Scholar] [CrossRef]
  59. McKenna, A.; Hanna, M.; Banks, E.; Sivachenko, A.; Cibulskis, K.; Kernytsky, A.; Garimella, K.; Altshuler, D.; Gabriel, S.; Daly, M.; et al. The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 2010, 20, 1297–1303. [Google Scholar] [CrossRef]
  60. Cingolani, P.; Platts, A.; Wang, L.L.; Coon, M.; Nguyen, T.; Wang, L.; Land, S.J.; Lu, X.; Ruden, D.M. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly 2012, 6, 80–92. [Google Scholar] [CrossRef]
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Figure 1. The hydroponic phenotypic of R178 (ATL) and S169 (ASL) under Al toxicity stress. Asterisks indicate significant differences between ATL and ASL (t test, ** p < 0.01).
Figure 1. The hydroponic phenotypic of R178 (ATL) and S169 (ASL) under Al toxicity stress. Asterisks indicate significant differences between ATL and ASL (t test, ** p < 0.01).
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Figure 2. The frequency distribution of RET of the F2:3 population under Al toxicity stress.
Figure 2. The frequency distribution of RET of the F2:3 population under Al toxicity stress.
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Figure 3. SNP-index distribution map on the whole genome. The black line is for the delta-SNP-index curve and the red line is the threshold at 99% confidence interval.
Figure 3. SNP-index distribution map on the whole genome. The black line is for the delta-SNP-index curve and the red line is the threshold at 99% confidence interval.
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Figure 4. The Indel-index distribution map on the whole genome. The black line is for the delta-Indel-index curve and the red line is the threshold at 99% confidence interval.
Figure 4. The Indel-index distribution map on the whole genome. The black line is for the delta-Indel-index curve and the red line is the threshold at 99% confidence interval.
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Table 1. Statistic analysis and normal distribution test of RET in the F2:3 population.
Table 1. Statistic analysis and normal distribution test of RET in the F2:3 population.
IndexesF2 NumberAverageMinimumMedianMaximumStandard DeviationCV/%KurtosisShapiro–WilkKolmogorov Smirnov
Value2740.6000.2810.5940.9300.14123.49−0.304p = 0.1441.000
Table 2. The SNP and Indel overlapping linked regions and genes.
Table 2. The SNP and Indel overlapping linked regions and genes.
ChromosomeQTLStartEndSize/MbGenes
chrA07qAT-A07-115,712,25418,799,7543.09542
chrA07qAT-A07-220,680,93120,755,0530.0712
chrA09qAT-A09-126,393,18327,036,2380.64102
Table 3. The candidate DEGs related Al-resistance screened by BSA-seq and RNA-seq.
Table 3. The candidate DEGs related Al-resistance screened by BSA-seq and RNA-seq.
Gene IDQTLFunction DescriptionR178S169R178 vs. S169
6 h vs. 0 h24 h vs. 0 h6 h vs. 0 h24 h vs. 0 h6 h24 h
BnaA07g20010DqAT-A07-1DeSI-like protein At4g17486−1.43-−1.59-1.59-
BnaA07g20030DqAT-A07-1Zinc finger protein 1−1.15−1.42−1.08---
BnaA07g20140DqAT-A07-1Vacuolar fusion protein CCZ1 homolog B----−1.67−1.48
BnaA07g20150DqAT-A07-1Chaperone protein dnaJ 8, chloroplastic----−2.52−2.49
BnaA07g20260DqAT-A07-1Protein ACTIVITY OF BC1 COMPLEX KINASE 3, chloroplastic----3.151.46
BnaA07g20290DqAT-A07-1Phosphoglycerate kinase 3, cytosolic----−1.71−1.48
BnaA07g20500DqAT-A07-1DNA repair protein recA homolog 1, chloroplastic----1.691.90
BnaA07g20510DqAT-A07-1Photosystem II 10 kDa polypeptide, chloroplastic--−1.89-3.051.17
BnaA07g20530DqAT-A07-1Interactor of constitutive active ROPs 41.08-1.28---
BnaA07g20740DqAT-A07-1Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, chloroplastic----−10.51−8.94
BnaA07g20870DqAT-A07-1Thaumatin-like protein----1.761.69
BnaA07g20960DqAT-A07-1Indole glucosinolate O-methyltransferase 1-1.22-1.191.891.53
BnaA07g21010DqAT-A07-1Ubinuclein-2----−1.82−2.43
BnaA07g21360DqAT-A07-1Increased DNA methylation 3----−1.15−1.22
BnaA07g21490DqAT-A07-1Dehydrin ERD14-−2.26-−2.19−1.10-
BnaA07g21500DqAT-A07-1PREDICTED: pro-resilin-like [Brassica rapa]----−1.16−1.08
BnaA07g21560DqAT-A07-1Rac-like GTP-binding protein ARAC5----2.722.71
BnaA07g21640DqAT-A07-1B-box zinc finger protein 21----−1.47−1.70
BnaA07g21850DqAT-A07-1Glycine--tRNA ligase, chloroplastic/mitochondrial 2----−1.52−1.33
BnaA07g21970DqAT-A07-1Protein MARD12.01-2.13---
BnaA07g22050DqAT-A07-1Protein NUCLEAR FUSION DEFECTIVE 4--1.35-−1.59−1.28
BnaA07g22100DqAT-A07-1Probable arabinosyltransferase ARAD1----−1.58−1.77
BnaA07g22220DqAT-A07-1GDSL esterase/lipase At1g74460 ----1.481.79
BnaA07g22620DqAT-A07-1Phosphoethanolamine N-methyltransferase 31.63--4.735.71-
BnaA07g22680DqAT-A07-1Sucrose synthase 6 --−3.04-3.612.44
BnaA07g22710DqAT-A07-1protein-lysine methyltransferase METTL21D [Brassica napus] ----−3.16−5.01
BnaA07g22740DqAT-A07-1Serine carboxypeptidase-like 2 --−1.40-−2.22−3.67
BnaA07g22770DqAT-A07-1F-box/LRR-repeat protein At5g02910 ----−3.62−2.69
BnaA07g22810DqAT-A07-1Anaphase-promoting complex subunit 13 ----2.522.25
BnaA07g22890DqAT-A07-1Beta-galactosidase 17 ---2.943.792.07
BnaA07g23200DqAT-A07-1Protein NRT1/ PTR FAMILY 5.11 ----2.252.37
BnaA07g23230DqAT-A07-1Protein NRT1/ PTR FAMILY 5.11 1.85-1.81-1.352.23
BnaA07g23320DqAT-A07-1ABC transporter G family member 25−1.59-−1.59-−2.57−2.51
BnaA07g23350DqAT-A07-1Sucrose transport protein SUC1 −2.21-−2.07−1.15−4.03−3.87
BnaA07g23650DqAT-A07-1Ethylene-responsive transcription factor ERF070 ----1.051.58
BnaA07g23760DqAT-A07-1Probable peroxygenase 4 ----−2.39−2.67
BnaA07g24010DqAT-A07-1Transcription factor KUA1 -−1.58−1.14--−1.44
BnaA07g24230DqAT-A07-1Cyclic dof factor 5 −5.40−1.09−6.03---
BnaA07g24270DqAT-A07-1NAC transcription factor 29 --−1.05−1.53−1.20−4.43
BnaA07g24310DqAT-A07-1Probable WRKY transcription factor 57 −1.16−1.48−2.11---
BnaA07g28730DqAT-A07-2Protein ALTERED XYL----1.081.02
BnaA07g28790DqAT-A07-2Probable peroxygenase 4 ----−3.11−3.13
BnaA09g36740DqAT-A09-1Pentatricopeptide repeat-containing protein At3g57430, chloroplastic ----−1.30−1.27
BnaA09g36900DqAT-A09-1protein enabled homolog [Brassica napus] ----1.101.19
BnaA09g36940DqAT-A09-1Probable glucuronoxylan glucuronosyltransferase F8H --−1.79-2.291.48
BnaA09g37240DqAT-A09-1E3 ubiquitin-protein ligase SINAT2 ----1.131.08
BnaA09g37360DqAT-A09-1MATH domain and coiled-coil domain-containing protein At3g58340 ----2.712.18
BnaA09g37400DqAT-A09-1Rhomboid-like protein 15 ----1.661.40
BnaA07g20780DqAT-A07-1PREDICTED: uncharacterized protein LOC1038305461.38-1.14---
BnaA07g21140DqAT-A07-1uncharacterized protein----−2.03−1.75
BnaA07g21190DqAT-A07-1uncharacterized protein----−9.48−8.96
BnaA07g23870DqAT-A07-1PREDICTED: uncharacterized protein LOC103830885 [Brassica rapa]----1.721.37
BnaA07g24050DqAT-A07-1uncharacterized protein−1.62−2.01--−1.01−1.24
BnaA07g24110DqAT-A07-1PREDICTED: uncharacterized protein LOC103830913 [Brassica rapa] −2.36---−2.762.66
BnaA09g36790DqAT-A09-1uncharacterized protein BNAA09G36790D [Brassica napus]----3.051.99
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Zhou, H.; Yu, P.; Wu, L.; Han, D.; Wu, Y.; Zheng, W.; Zhou, Q.; Xiao, X. Combined BSA-Seq and RNA-Seq Analysis to Identify Candidate Genes Associated with Aluminum Toxicity in Rapeseed (Brassica napus L.). Int. J. Mol. Sci. 2024, 25, 11190. https://doi.org/10.3390/ijms252011190

AMA Style

Zhou H, Yu P, Wu L, Han D, Wu Y, Zheng W, Zhou Q, Xiao X. Combined BSA-Seq and RNA-Seq Analysis to Identify Candidate Genes Associated with Aluminum Toxicity in Rapeseed (Brassica napus L.). International Journal of Molecular Sciences. 2024; 25(20):11190. https://doi.org/10.3390/ijms252011190

Chicago/Turabian Style

Zhou, Huiwen, Paolan Yu, Lanhua Wu, Depeng Han, Yang Wu, Wei Zheng, Qinghong Zhou, and Xiaojun Xiao. 2024. "Combined BSA-Seq and RNA-Seq Analysis to Identify Candidate Genes Associated with Aluminum Toxicity in Rapeseed (Brassica napus L.)" International Journal of Molecular Sciences 25, no. 20: 11190. https://doi.org/10.3390/ijms252011190

APA Style

Zhou, H., Yu, P., Wu, L., Han, D., Wu, Y., Zheng, W., Zhou, Q., & Xiao, X. (2024). Combined BSA-Seq and RNA-Seq Analysis to Identify Candidate Genes Associated with Aluminum Toxicity in Rapeseed (Brassica napus L.). International Journal of Molecular Sciences, 25(20), 11190. https://doi.org/10.3390/ijms252011190

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