Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation
Abstract
:1. Introduction
2. Results
2.1. YB-1 and YB-3 Expressions Partially Restore Translation in HEK293TΔYB-1ΔYB-3 Cells
2.2. Translational Changes Correlate in YB-1- and YB-3-Expressing Cells
2.3. Translationally Altered Genes in YB-1- and YB-3-Expressing Cells Participate in Similar Biological Processes
3. Discussion
4. Materials and Methods
4.1. Plasmids
4.2. Cell Cultures
4.3. Western Blot and Antibodies
4.4. Sucrose Gradient
4.5. Ribo-Seq and RNA-Seq
4.6. PAR-CLIP
4.7. High-Throughput Sequencing and Data Processing
4.8. PAR-CLIP and eCLIP Data Processing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Eliseeva, I.A.; Buyan, A.I.; Smolin, E.A.; Kaliadzenka, K.S.; Popov, S.; Kulakovskiy, I.V.; Lyabin, D.N. Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation. Int. J. Mol. Sci. 2024, 25, 1736. https://doi.org/10.3390/ijms25031736
Eliseeva IA, Buyan AI, Smolin EA, Kaliadzenka KS, Popov S, Kulakovskiy IV, Lyabin DN. Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation. International Journal of Molecular Sciences. 2024; 25(3):1736. https://doi.org/10.3390/ijms25031736
Chicago/Turabian StyleEliseeva, Irina A., Andrey I. Buyan, Egor A. Smolin, Karina S. Kaliadzenka, Sergey Popov, Ivan V. Kulakovskiy, and Dmitry N. Lyabin. 2024. "Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation" International Journal of Molecular Sciences 25, no. 3: 1736. https://doi.org/10.3390/ijms25031736