Evidence of the Role of Inflammation and the Hormonal Environment in the Pathogenesis of Adrenal Myelolipomas in Congenital Adrenal Hyperplasia
Abstract
:1. Introduction
2. Results
2.1. Characteristics of the CAH Study Population
2.2. Histological Features of CAH Adrenal Myelolipoma
2.3. Gene Expression of Inflammatory and Lipogenic Genes by qRT-PCR
2.4. RNA-Sequencing Analysis
2.5. Cell Chemotactic Assay
3. Discussion
4. Materials and Methods
4.1. Tissue Collection
4.2. Histology and Immunohistochemistry
4.3. Gene Expression Analysis by qRT-PCR
4.4. Cell Culture
4.5. RNA-Sequencing and Differential Expression Testing
4.6. In Vitro Invasion and Transfilter Migration Assays
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kolli, V.; Frucci, E.; da Cunha, I.W.; Iben, J.R.; Kim, S.A.; Mallappa, A.; Li, T.; Faucz, F.R.; Kebebew, E.; Nilubol, N.; et al. Evidence of the Role of Inflammation and the Hormonal Environment in the Pathogenesis of Adrenal Myelolipomas in Congenital Adrenal Hyperplasia. Int. J. Mol. Sci. 2024, 25, 2543. https://doi.org/10.3390/ijms25052543
Kolli V, Frucci E, da Cunha IW, Iben JR, Kim SA, Mallappa A, Li T, Faucz FR, Kebebew E, Nilubol N, et al. Evidence of the Role of Inflammation and the Hormonal Environment in the Pathogenesis of Adrenal Myelolipomas in Congenital Adrenal Hyperplasia. International Journal of Molecular Sciences. 2024; 25(5):2543. https://doi.org/10.3390/ijms25052543
Chicago/Turabian StyleKolli, Vipula, Emily Frucci, Isabela Werneck da Cunha, James R. Iben, Sun A. Kim, Ashwini Mallappa, Tianwei Li, Fabio Rueda Faucz, Electron Kebebew, Naris Nilubol, and et al. 2024. "Evidence of the Role of Inflammation and the Hormonal Environment in the Pathogenesis of Adrenal Myelolipomas in Congenital Adrenal Hyperplasia" International Journal of Molecular Sciences 25, no. 5: 2543. https://doi.org/10.3390/ijms25052543