The Prolonged Activation of the p65 Subunit of the NF-Kappa-B Nuclear Factor Sustains the Persistent Effect of Advanced Glycation End Products on Inflammatory Sensitization in Macrophages
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Isolation of Lipoproteins
4.2. LDL Acetylation
4.3. Advanced Glycation of Albumin In Vitro
4.4. Culture of RAW 264.7 Macrophages
4.5. Determining the Persistence Time of the Effect of AGE-Albumin Effect on Macrophages via NFKB Activation
4.6. Gene Expression Analysis of Nfkb1, RelA, Il6, Tnf, Abca1, Tlr4, and Ager
4.7. Determination of Protein Content by Western Blot
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Assis, S.I.S.d.; Amendola, L.S.; Okamoto, M.M.; Ferreira, G.d.S.; Iborra, R.T.; Santos, D.R.; Santana, M.d.F.M.; Santana, K.G.; Correa-Giannella, M.L.; Barbeiro, D.F.; et al. The Prolonged Activation of the p65 Subunit of the NF-Kappa-B Nuclear Factor Sustains the Persistent Effect of Advanced Glycation End Products on Inflammatory Sensitization in Macrophages. Int. J. Mol. Sci. 2024, 25, 2713. https://doi.org/10.3390/ijms25052713
Assis SISd, Amendola LS, Okamoto MM, Ferreira GdS, Iborra RT, Santos DR, Santana MdFM, Santana KG, Correa-Giannella ML, Barbeiro DF, et al. The Prolonged Activation of the p65 Subunit of the NF-Kappa-B Nuclear Factor Sustains the Persistent Effect of Advanced Glycation End Products on Inflammatory Sensitization in Macrophages. International Journal of Molecular Sciences. 2024; 25(5):2713. https://doi.org/10.3390/ijms25052713
Chicago/Turabian StyleAssis, Sayonara Ivana Santos de, Leonardo Szalo Amendola, Maristela Mitiko Okamoto, Guilherme da Silva Ferreira, Rodrigo Tallada Iborra, Danielle Ribeiro Santos, Monique de Fátima Mello Santana, Kelly Gomes Santana, Maria Lucia Correa-Giannella, Denise Frediani Barbeiro, and et al. 2024. "The Prolonged Activation of the p65 Subunit of the NF-Kappa-B Nuclear Factor Sustains the Persistent Effect of Advanced Glycation End Products on Inflammatory Sensitization in Macrophages" International Journal of Molecular Sciences 25, no. 5: 2713. https://doi.org/10.3390/ijms25052713