Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an Engineered Strain of Streptomyces albidoflavus
Abstract
:1. Introduction
2. Results
2.1. Heterologous Biosynthesis of Diosmetin
2.2. Heterologous Biosynthesis of Chrysoeriol
2.3. Identification of Putative Luteolin 3′,4′-Dimethyl Ether in Both Diosmetin- and Chrysoeriol-Producing Strains
2.4. Use of a DAHP Synthase to Increase the Production Titers of Diosmetin and Chrysoeriol through Precursor Titer Enhancement
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Culture Conditions
Description | Reference | |
---|---|---|
Plasmids | ||
pSEVA181-At4g26220 | Source of At4g26220 (Level 0 MoClo) | This study |
pSEVA181SP25 | Source of SP25 (Level 0 MoClo) | [21] |
pSEVA181SP43 | Source of SP43 (Level 0 MoClo) | [21] |
pSEVA181-M444_29925 | Source of M444_29925 (Level 0 MoClo) | This study |
pSEVA181RiboJ-RBS | Source of RiboJ-RBS (Level 0 MoClo) | [21] |
pIDTSMARTttsbib | Source of ttsbib (Level 0 MoClo) | [21] |
pSEVAUO-M21102 | Level 2 MoClo receptor | [21] |
pSEVAUO-M31205 | Level 2 MoClo receptor | [21] |
pSEVAUO-M21206F3H-CPR | Level 1 MoClo harboring F3′H-CPR | [21] |
PCR-Blunt II-TOPO-FNS1 | Source of FNS1 (Level 0 MoClo) | [24] |
pSEVAUO-M21102-FNS1 | Level 1 MoClo harboring FNS1 | This study |
pSEVAUO-M21503-FNS1/F3′H-CPR | Level 2 MoClo harboring FNS1 and F3′H-CPR | This study |
pSEVAUO-M31105-At4g26220 | Level 1 MoClo plasmid harboring At4g26220 | This study |
pSEVAUO-M31105-M444_29925 | Level 1 MoClo plasmid harboring M444_29925 | This study |
pSEVAUO-M31105 | Level 1 MoClo receptor | [21] |
pSEVAUO-M31205-dahp | Level 1 MoClo plasmid harboring dahp | This study |
pSEVAUO-M31505 | Level 2 MoClo receptor | [21] |
pSEVAUO-M31505-At4g26220-dahp | Level 2 MoClo harboring At4g26220 and dahp | This study |
pSEVAUO-M31505-M444_29925-dahp | Level 2 MoClo harboring M444_29925 and dahp | This study |
Strains | ||
E. coli TOP10 | Strain used for routine subcloning | Invitrogen (Waltham, MA, USA) |
E. coli ET12567/pUZ8002 | Strain used for conjugation | [48] |
UO-FLAV-004 | S. albidoflavus strain used in this work | [24] |
UO-FLAV-004-NAR | UO-FLAV-004 harboring TAL, 4CL, CHS and CHI | [24] |
UO-FLAV-004-LUT | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1 and F3′H-CPR | This study |
UO-FLAV-004-DIO | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1, F3′H-CPR and M444_29925 | This study |
UO-FLAV-004-CHR | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1, F3′H-CPR and At4g26220 | This study |
UO-FLAV-004-DIO-dahp | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1, F3′H-CPR, M444_29925 and dahp | This study |
UO-FLAV-004-CHR-dahp | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1, F3′H-CPR, At4g26220 and dahp | This study |
UO-FLAV-004-FNS1 | UO-FLAV-004 harboring FNS1 | [24] |
4.2. Reagents and Biochemicals
4.3. Genes and Enzymes
4.4. Plasmids Construction
4.4.1. Construction of pSEVAUO-M21503-FNS1/F3′H-CPR
4.4.2. Construction of pSEVAUO-M31105-At4g26220, pSEVAUO-M31105-M444_29925, and pSEVAUO-M31205-dahp
4.4.3. Construction of pSEVAUO-M31505-At4g26220-dahp and pSEVAUO-M31505-M444_29925-dahp
4.5. Flavonoid Extraction and LC-DAD Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pérez-Valero, Á.; Serna-Diestro, J.; Villar, C.J.; Lombó, F. Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an Engineered Strain of Streptomyces albidoflavus. Int. J. Mol. Sci. 2024, 25, 2776. https://doi.org/10.3390/ijms25052776
Pérez-Valero Á, Serna-Diestro J, Villar CJ, Lombó F. Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an Engineered Strain of Streptomyces albidoflavus. International Journal of Molecular Sciences. 2024; 25(5):2776. https://doi.org/10.3390/ijms25052776
Chicago/Turabian StylePérez-Valero, Álvaro, Juan Serna-Diestro, Claudio J. Villar, and Felipe Lombó. 2024. "Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an Engineered Strain of Streptomyces albidoflavus" International Journal of Molecular Sciences 25, no. 5: 2776. https://doi.org/10.3390/ijms25052776