Cambium Reactivation Is Closely Related to the Cell-Cycle Gene Configuration in Larix kaempferi
Abstract
:1. Introduction
2. Results
2.1. Low-Temperature Treatment Accelerates Bud Break in Seedlings Overall
2.2. Cell-Cycle Genes Are Regulated by Temperature
2.3. Water Culture Induces Cambium Reactivation and Cell-Cycle Gene Expression in L. kaempferi
2.4. Cambium Reactivation Is Closely Related to the Cell-Cycle Gene Configuration
3. Discussion
3.1. Chilling Induces the Transition of L. kaempferi from the Rest to the Quiescence Stage
3.2. Spring Temperatures Induce Cell-Cycle Gene Expression in L. kaempferi
3.3. Cell-Cycle Genes Cooperatively Regulate Cell Division
3.4. Age Affects the Timing of Bud Reactivation and Orchestrates the Configuration Process of Cell-Cycle Genes
4. Materials and Methods
4.1. Sample Treatment and Collection
4.1.1. Natural Chilling Treatment Experiments
4.1.2. Materials Collected from the Active and Dormant L. kaempferi Trees
4.1.3. Materials Collected during Natural Reactivation of L. kaempferi Trees
4.1.4. Water Culture Experiments
4.2. RNA Extraction and cDNA Synthesis
4.3. qRT-PCR
4.4. Anatomical Observations of Secondary Vascular Tissue
4.5. Yeast Two-Hybrid Assay
4.6. Bimolecular Fluorescence Complementation Assay
4.7. Relationship between Cell-Cycle Gene Expression and Temperature
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment Time (Days) | Number of Seedlings | Number of Seedlings with Bud Break | Number of Seedlings with No Bud Break | Range of Bud Break Time (Day) | Average Bud Break Time (Day) | Standard Deviation of Bud Break Time | |
---|---|---|---|---|---|---|---|
Low Temperature | Warm Temperature | ||||||
0 | 93 | 10 | 9 | 1 | 29–91 | 52 | 19.5 |
5 | 88 | 10 | 5 | 5 | 24–78 | 56 | 20.1 |
10 | 83 | 10 | 7 | 3 | 17–78 | 49 | 24.5 |
15 | 78 | 10 | 3 | 7 | 48–77 | 65 | 15.3 |
20 | 73 | 10 | 8 | 2 | 9–73 | 32 | 21.2 |
25 | 68 | 10 | 8 | 2 | 5–65 | 28 | 21.1 |
30 | 63 | 10 | 9 | 1 | 6–34 | 14 | 8.9 |
35 | 58 | 10 | 9 | 1 | 5–18 | 10 | 4.4 |
39 | 54 | 10 | 10 | 0 | 4–40 | 11 | 10.5 |
44 | 49 | 8 | 8 | 0 | 4–18 | 10 | 5.1 |
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Cheng, D.-X.; Wang, X.-H.; Wang, C.-L.; Li, X.-Y.; Ye, Z.-L.; Li, W.-F. Cambium Reactivation Is Closely Related to the Cell-Cycle Gene Configuration in Larix kaempferi. Int. J. Mol. Sci. 2024, 25, 3578. https://doi.org/10.3390/ijms25073578
Cheng D-X, Wang X-H, Wang C-L, Li X-Y, Ye Z-L, Li W-F. Cambium Reactivation Is Closely Related to the Cell-Cycle Gene Configuration in Larix kaempferi. International Journal of Molecular Sciences. 2024; 25(7):3578. https://doi.org/10.3390/ijms25073578
Chicago/Turabian StyleCheng, Dong-Xia, Xin-Hao Wang, Cong-Li Wang, Xiang-Yi Li, Zha-Long Ye, and Wan-Feng Li. 2024. "Cambium Reactivation Is Closely Related to the Cell-Cycle Gene Configuration in Larix kaempferi" International Journal of Molecular Sciences 25, no. 7: 3578. https://doi.org/10.3390/ijms25073578