High NaCl Concentrations in Water Are Associated with Developmental Abnormalities and Altered Gene Expression in Zebrafish
Abstract
:1. Introduction
2. Results
2.1. Impact of Increased Salt Concentrations on Zebrafish Development from the Blastocyst to Larval Stage
2.2. Impact of Increased Salt Concentrations on Zebrafish Development from the Two-Cell to Blastocyst Stage
2.3. Altered Expression of Sonic Hedgehog Pathway Genes in Zebrafish Embryos Treated with Higher NaCl Concentrations
2.4. Transcriptomic Landscape of Zebrafish Embryos Treated with Higher NaCl Concentrations
3. Discussion
4. Materials and Methods
4.1. Zebrafish Culture
4.2. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR)
4.3. Statistical Analyses
4.4. RNA Extraction and Sequencing
4.5. RNA Sequencing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Seli, D.A.; Prendergast, A.; Ergun, Y.; Tyagi, A.; Taylor, H.S. High NaCl Concentrations in Water Are Associated with Developmental Abnormalities and Altered Gene Expression in Zebrafish. Int. J. Mol. Sci. 2024, 25, 4104. https://doi.org/10.3390/ijms25074104
Seli DA, Prendergast A, Ergun Y, Tyagi A, Taylor HS. High NaCl Concentrations in Water Are Associated with Developmental Abnormalities and Altered Gene Expression in Zebrafish. International Journal of Molecular Sciences. 2024; 25(7):4104. https://doi.org/10.3390/ijms25074104
Chicago/Turabian StyleSeli, Denis A., Andrew Prendergast, Yagmur Ergun, Antariksh Tyagi, and Hugh S. Taylor. 2024. "High NaCl Concentrations in Water Are Associated with Developmental Abnormalities and Altered Gene Expression in Zebrafish" International Journal of Molecular Sciences 25, no. 7: 4104. https://doi.org/10.3390/ijms25074104