Genome-Wide Identification of the WRKY Gene Family and Functional Characterization of CpWRKY5 in Cucurbita pepo
Abstract
:1. Introduction
2. Results
2.1. Identification and Evaluation of Chromosomal Location, Multiple Sequence Alignment, and Phylogenetic Relationships of CpWRKY Proteins
2.2. Gene Structure and Conserved Motif Analysis of CpWRKYs
2.3. Synteny and Collinearity Analyses
2.4. Analysis of Cis-Acting Elements in the Promoters of CpWRKYs
2.5. Expression Analysis of WRKY TFs of Seed Coat Development in C. pepo
2.6. CpWRKY5 Positively Regulates Lignin Synthesis in Transgenic Tobacco
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification and Characterization of Putative WRKY Genes in C. pepo
4.3. Sequence Alignment, Phylogenetic Tree, Classification, Gene Structure, and Conserved Motif Analyse
4.4. Chromosomal Location, Gene Duplication, Collinearity Analyses, and Cis-Acting Elements in the Promoters of the CpWRKY Family Genes
4.5. Gene Expression Analysis
4.6. Construction of Transgenic Nicotiana tabacum Overexpressing CpWRKY5
4.7. Determination of Lignin Content and Related Gene Expression
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, J.; Tao, F.; Xue, Y.; Xu, B.; Li, X. Genome-Wide Identification of the WRKY Gene Family and Functional Characterization of CpWRKY5 in Cucurbita pepo. Int. J. Mol. Sci. 2024, 25, 4177. https://doi.org/10.3390/ijms25084177
Chen J, Tao F, Xue Y, Xu B, Li X. Genome-Wide Identification of the WRKY Gene Family and Functional Characterization of CpWRKY5 in Cucurbita pepo. International Journal of Molecular Sciences. 2024; 25(8):4177. https://doi.org/10.3390/ijms25084177
Chicago/Turabian StyleChen, Junhong, Fei Tao, Yingyu Xue, Bingliang Xu, and Xiaowei Li. 2024. "Genome-Wide Identification of the WRKY Gene Family and Functional Characterization of CpWRKY5 in Cucurbita pepo" International Journal of Molecular Sciences 25, no. 8: 4177. https://doi.org/10.3390/ijms25084177