Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum
Abstract
:1. Introduction
2. Results
2.1. Effects of Cancer Patient Serum on Human Skeletal Muscle Differentiation and Atrophy In Vitro
2.2. Establishment of a High-Content Phenotypic Screening System Using Cancer Patient Serum for Drug Screening
2.3. Drug Screening Using High-Content Phenotypic Screening with Cancer Patient Sera
2.4. The Effects of AR-42 on Muscle Differentiation Inhibition by Cancer Patient Sera
3. Discussion
4. Materials and Methods
4.1. Human Cell Culture and Evaluation of Skeletal Muscle Differentiation and Atrophy
4.2. High Content, Phenotypic Screening
4.3. Immunostaining and Fluorescence Analysis
4.4. Quantitative Real Time PCR
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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¥ | Patient Diagnosis | Grade | TNM Classification |
---|---|---|---|
A | – * | – * | – * |
B | – * | – * | – * |
C | Lung Cancer | III | T3, N0, M0 |
D | Lung Cancer | IIIA | T2, N2, M0 |
E | Colon Cancer | III | T3, Nx, M0 |
F | Colon Cancer | III | T3, N0, M0 |
G | Pancreatic Cancer | IV | Unknown |
H | Pancreatic Cancer | IV | Unknown |
I | Stomach Cancer | IV | T3, Nx, M1 |
J | Stomach Cancer | Unknown | Unknown |
K | Pancreatic Cancer | III | T4, N0, M0 |
L | Pancreatic Cancer | III | T4, N0, M0 |
M | Pancreatic Cancer | IV | Unknown |
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Nakane, A.; Nakagawa, H.; Nagata, H. Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum. Pharmaceuticals 2025, 18, 445. https://doi.org/10.3390/ph18040445
Nakane A, Nakagawa H, Nagata H. Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum. Pharmaceuticals. 2025; 18(4):445. https://doi.org/10.3390/ph18040445
Chicago/Turabian StyleNakane, Atsushi, Hiroyuki Nakagawa, and Hidetaka Nagata. 2025. "Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum" Pharmaceuticals 18, no. 4: 445. https://doi.org/10.3390/ph18040445
APA StyleNakane, A., Nakagawa, H., & Nagata, H. (2025). Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum. Pharmaceuticals, 18(4), 445. https://doi.org/10.3390/ph18040445