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Article

Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy

by
Fei Cao
1,†,
Xintong Wang
1,†,
Qingqing Ye
1,
Fang Yan
1,
Weicheng Lu
1,
Jingdun Xie
1,
Bingtian Bi
2,* and
Xudong Wang
1,*
1
Department of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, China
2
Department of Clinical Trial Center, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2023, 45(8), 6804-6822; https://doi.org/10.3390/cimb45080430
Submission received: 18 July 2023 / Revised: 13 August 2023 / Accepted: 15 August 2023 / Published: 16 August 2023
(This article belongs to the Special Issue Studying the Function of RNAs Using Omics Approaches)
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Abstract

Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and severe side effect of first-line chemotherapeutic agents. The association between circular RNAs (circRNAs) and CIPN remains unclear. In this study, CIPN models were constructed with Taxol, while 134 differentially expressed circRNAs, 353 differentially expressed long non-coding RNAs, and 86 differentially expressed messenger RNAs (mRNAs) were identified utilizing RNA sequencing. CircRNA-targeted microRNAs (miRNAs) were predicted using miRanda, and miRNA-targeted mRNAs were predicted using TargetScan and miRDB. The intersection of sequencing and mRNA prediction results was selected to establish the circRNA–miRNA–mRNA networks, which include 15 circRNAs, 18 miRNAs, and 11 mRNAs. Functional enrichment pathway analyses and immune infiltration analyses revealed that differentially expressed mRNAs were enriched in the immune system, especially in T cells, monocytes, and macrophages. Cdh1, Satb2, Fas, P2ry2, and Zfhx2 were further identified as hub genes and validated by RT-qPCR, correlating with macrophages, plasmacytoid dendritic cells, and central memory CD4 T cells in CIPN. Additionally, we predicted the associated diseases, 36 potential transcription factors (TFs), and 30 putative drugs for hub genes using the DisGeNET, TRRUST, and DGIdb databases, respectively. Our results indicated the crucial role of circRNAs, and the immune microenvironment played in CIPN, providing novel insights for further research.
Keywords: chemotherapy-induced peripheral neuropathy; circular RNA; ceRNA; immune infiltration; regulatory network chemotherapy-induced peripheral neuropathy; circular RNA; ceRNA; immune infiltration; regulatory network

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MDPI and ACS Style

Cao, F.; Wang, X.; Ye, Q.; Yan, F.; Lu, W.; Xie, J.; Bi, B.; Wang, X. Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy. Curr. Issues Mol. Biol. 2023, 45, 6804-6822. https://doi.org/10.3390/cimb45080430

AMA Style

Cao F, Wang X, Ye Q, Yan F, Lu W, Xie J, Bi B, Wang X. Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy. Current Issues in Molecular Biology. 2023; 45(8):6804-6822. https://doi.org/10.3390/cimb45080430

Chicago/Turabian Style

Cao, Fei, Xintong Wang, Qingqing Ye, Fang Yan, Weicheng Lu, Jingdun Xie, Bingtian Bi, and Xudong Wang. 2023. "Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy" Current Issues in Molecular Biology 45, no. 8: 6804-6822. https://doi.org/10.3390/cimb45080430

APA Style

Cao, F., Wang, X., Ye, Q., Yan, F., Lu, W., Xie, J., Bi, B., & Wang, X. (2023). Identifying circRNA–miRNA–mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy. Current Issues in Molecular Biology, 45(8), 6804-6822. https://doi.org/10.3390/cimb45080430

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