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Article

The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells

by
Sergey S. Soroko
1,
Dmitry V. Skamnitskiy
2,
Ekaterina N. Gorshkova
1,
Olga M. Kutova
1,
Ismail R. Seriev
1,
Anna V. Maslennikova
1,2,3,
Evgeniy L. Guryev
1,
Sergey V. Gudkov
1,4,5,
Vladimir A. Vodeneev
1,
Irina V. Balalaeva
1 and
Natalia Yu Shilyagina
1,*
1
Department of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, Russia
2
Nizhniy Novgorod Regional Oncology Hospital, St. Rodionova, 190, 603950 Nizhny Novgorod, Russia
3
Department of Oncology, Radiation Therapy and Radiation Diagnostics, Privolzhsky Research Medical University, Minin and Pozharsky Sq., 10/1, 603950 Nizhny Novgorod, Russia
4
Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilov Str. 38, 119991 Moscow, Russia
5
Federal Scientific Agronomic and Engineering Center VIM, 1st Institutsky Proezd 5, 109428 Moscow, Russia
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2024, 46(12), 13860-13880; https://doi.org/10.3390/cimb46120828
Submission received: 25 October 2024 / Revised: 29 November 2024 / Accepted: 2 December 2024 / Published: 6 December 2024
(This article belongs to the Special Issue Molecular Insights into Radiation Oncology)
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Abstract

Modern radiotherapy utilizes a broad range of sources of ionizing radiation, both low-dose-rate (LDR) and high-dose-rate (HDR). However, the mechanisms underlying specific dose-rate effects remain unclear, especially for corpuscular radiation. To address this issue, we have irradiated human epidermoid carcinoma A431 cells under LDR and HDR regimes. Reducing the dose rate has lower lethality at equal doses with HDR irradiation. The half-lethal dose after HDR irradiation was three times less than after LDR irradiation. The study of mechanisms showed that under HDR irradiation, the radiation-induced halt of mitosis with the accompanying emergence of giant cells was recorded. No such changes were recorded after LDR irradiation. The level of DNA damage is significantly greater after HDR irradiation, which may be the main reason for the different mechanisms of action of HDR and LDR irradiations. Comparing the mechanisms of cell response to LDR and HDR irradiations may shed light on the mechanisms of tumor cell response to ionizing radiation and answer the question of whether different dose rates within the same dose range can cause different clinical effects.
Keywords: ionizing radiation; radiotherapy; cancer; low dose rate; high dose rate; mechanism cell response; beta-radiation; cell cycle; cell death; apoptosis; ROS; senescence; giant cells ionizing radiation; radiotherapy; cancer; low dose rate; high dose rate; mechanism cell response; beta-radiation; cell cycle; cell death; apoptosis; ROS; senescence; giant cells
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MDPI and ACS Style

Soroko, S.S.; Skamnitskiy, D.V.; Gorshkova, E.N.; Kutova, O.M.; Seriev, I.R.; Maslennikova, A.V.; Guryev, E.L.; Gudkov, S.V.; Vodeneev, V.A.; Balalaeva, I.V.; et al. The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells. Curr. Issues Mol. Biol. 2024, 46, 13860-13880. https://doi.org/10.3390/cimb46120828

AMA Style

Soroko SS, Skamnitskiy DV, Gorshkova EN, Kutova OM, Seriev IR, Maslennikova AV, Guryev EL, Gudkov SV, Vodeneev VA, Balalaeva IV, et al. The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells. Current Issues in Molecular Biology. 2024; 46(12):13860-13880. https://doi.org/10.3390/cimb46120828

Chicago/Turabian Style

Soroko, Sergey S., Dmitry V. Skamnitskiy, Ekaterina N. Gorshkova, Olga M. Kutova, Ismail R. Seriev, Anna V. Maslennikova, Evgeniy L. Guryev, Sergey V. Gudkov, Vladimir A. Vodeneev, Irina V. Balalaeva, and et al. 2024. "The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells" Current Issues in Molecular Biology 46, no. 12: 13860-13880. https://doi.org/10.3390/cimb46120828

APA Style

Soroko, S. S., Skamnitskiy, D. V., Gorshkova, E. N., Kutova, O. M., Seriev, I. R., Maslennikova, A. V., Guryev, E. L., Gudkov, S. V., Vodeneev, V. A., Balalaeva, I. V., & Shilyagina, N. Y. (2024). The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells. Current Issues in Molecular Biology, 46(12), 13860-13880. https://doi.org/10.3390/cimb46120828

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