Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Lonicera japonica Thunb. Extracts
2.2. Cell Line and Cell Culture
2.3. Matrix Metallopeptidase (MMP) Zymography
2.4. Cell Counting Kit-8 (CCK-8) Assay
2.5. Quantitative Reverse Transcription–PCR (qRT-PCR)
2.6. Western Blot Analysis
2.7. Cell Migration Assay
2.8. Immunofluorescence Stain
2.9. Luciferase Assay
2.10. ELISA
2.11. Statistical Analysis
3. Results
3.1. COX-2 and MMP-9 Expression upon TNF-α Treatment in GES-1 Cells
3.2. Effect of Lonicera japonica Thunb. Ethanol Extract on the Expressions of TNF-α-Induced COX-2, PGE2, and MMP-9 in GES-1 Cells
3.3. The Role of ERK1/2 and c-Fos in TNF-α-Stimulated COX-2 and MMP-9 Expression in Normal GES-1 Cells
3.4. The Effect of NF-κB on TNF-α-Stimulated COX-2 and MMP-9 Expression in Normal GES-1 Cells
3.5. The Antimetastatic Activity of Ethanol Extract from Lonicera japonica Thunb. Was Evaluated In Vitro
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Hsieh, H.-L.; Yu, M.-C.; Chang, Y.-C.; Wu, Y.-H.; Huang, K.-H.; Tsai, M.-M. Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9. Curr. Issues Mol. Biol. 2024, 46, 7303-7323. https://doi.org/10.3390/cimb46070433
Hsieh H-L, Yu M-C, Chang Y-C, Wu Y-H, Huang K-H, Tsai M-M. Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9. Current Issues in Molecular Biology. 2024; 46(7):7303-7323. https://doi.org/10.3390/cimb46070433
Chicago/Turabian StyleHsieh, Hsi-Lung, Ming-Chin Yu, Yu-Chia Chang, Yi-Hsuan Wu, Kuo-Hsiung Huang, and Ming-Ming Tsai. 2024. "Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9" Current Issues in Molecular Biology 46, no. 7: 7303-7323. https://doi.org/10.3390/cimb46070433