A Bioinformatic Analysis Predicts That Cannabidiol Could Function as a Potential Inhibitor of the MAPK Pathway in Colorectal Cancer

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

I have read the submission entitled 'Cannabidiol acts as a predicted inhibitor of the MAPK pathway in colorectal cancer'. The study is well written and design good. However, the result in the present form is too less. I suggest Western blotting and PCR should added in this study to prove the MAPK pathway.

Author Response

Comment 1: I have read the submission entitled 'Cannabidiol acts as a predicted inhibitor of the MAPK pathway in colorectal cancer'. The study is well written and design good. However, the result in the present form is too less. I suggest Western blotting and PCR should added in this study to prove the MAPK pathway.

Response 1: We have read the comment and as we do agree that in vitro validation is needed, it is not feasible to do it within the short time frame. This paper is part of my MSc degree so in vitro target validation will only be able to be submitted at a later stages. Therefore no changes has been made to the present manuscript as the intent was always for this to be a purely bioinformatic paper, with in vitro results being added in a second paper. 

Reviewer 2 Report

Comments and Suggestions for Authors

Computation studies are accompanied enzymatic or receptor assays that are important in searching for inhibitors that can serve as potential drugs in fight against cancer and it helps to understand their physicochemicals properties AFTER show by in vivo assays that examined compound (here CBD) inhibit all or some of elements of EGFR and MAPK pathways – presented MS lack of most important part of study. Silico study cannot guarantee the same action in real life, so they are highly speculative. 

Author Response

Comment 1: Computation studies are accompanied enzymatic or receptor assays that are important in searching for inhibitors that can serve as potential drugs in fight against cancer and it helps to understand their physicochemicals properties AFTER show by in vivo assays that examined compound (here CBD) inhibit all or some of elements of EGFR and MAPK pathways – presented MS lack of most important part of study. Silico study cannot guarantee the same action in real life, so they are highly speculative. 

Response 1: We have read the comment and as we do agree that in vitro validation is needed, it is not feasible to do it within the short time frame. This paper is part of my MSc degree so in vitro target validation will only be able to be submitted at a later stages. Therefore no changes has been made to the present manuscript as the intent was always for this to be a purely bioinformatic paper, with in vitro results being added in a second paper. 

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

MS is written very well although I strongly think that in vitro enzymatic assays should be added as part of study. As Editor accept MS to revision and as this part is presented very well I can agree with Editor decision to accept MS.  

Author Response

Comments 1: MS is written very well although I strongly think that in vitro enzymatic assays should be added as part of study. As Editor accept MS to revision and as this part is presented very well I can agree with Editor decision to accept MS.  

Response 1: The suggestion from the editor to change/alter the title has been accepted and changed accordingly.

Author Response File: Author Response.docx