TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture and Image-Based Fecal Metabolite Library Screening
2.3. Migrasome Counting and Live Cell Imaging
2.4. Western Blot Analysis
2.5. Statistical Analyses
3. Results and Discussion
3.1. Cadaverine and Putrescine Promotes Migrasome in RPE/GFP-TSPAN4 Cells
3.2. Cadaverine and Putrescine Inhibit PKA Phosphorylation in RPE Cells
3.3. TAAR8 Knock-Down Inhibits Cadaverine-Inducing Migrasome Formation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kim, J.B.; Bae, J.-E.; Park, N.Y.; Kim, Y.H.; Kim, S.H.; Hyung, H.; Yeom, E.; Choi, D.K.; Jeong, K.; Cho, D.-H. TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells. Curr. Issues Mol. Biol. 2024, 46, 8658-8664. https://doi.org/10.3390/cimb46080510
Kim JB, Bae J-E, Park NY, Kim YH, Kim SH, Hyung H, Yeom E, Choi DK, Jeong K, Cho D-H. TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells. Current Issues in Molecular Biology. 2024; 46(8):8658-8664. https://doi.org/10.3390/cimb46080510
Chicago/Turabian StyleKim, Joon Bum, Ji-Eun Bae, Na Yeon Park, Yong Hwan Kim, Seong Hyun Kim, Hyejin Hyung, Eunbyul Yeom, Dong Kyu Choi, Kwiwan Jeong, and Dong-Hyung Cho. 2024. "TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells" Current Issues in Molecular Biology 46, no. 8: 8658-8664. https://doi.org/10.3390/cimb46080510