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Article

Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer

by
Dwi Anita Suryandari
1,2,*,
Miftahuzzakiyah Miftahuzzakiyah
3,
Luluk Yunaini
1,
Ria Kodariah
2,4,
Dewi Sukmawati
5,
Primariadewi Rustamadji
4,
Puji Sari
1 and
Sri Suciati Ningsih
6
1
Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
2
Biobank Research IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
3
Master Program in Biomedical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
4
Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
5
Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
6
Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2025, 47(2), 78; https://doi.org/10.3390/cimb47020078
Submission received: 3 December 2024 / Revised: 18 January 2025 / Accepted: 19 January 2025 / Published: 26 January 2025
(This article belongs to the Section Molecular Medicine)
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Abstract

AKNA is identified as a gene that regulates inflammation, immune response, and Epithelial–Mesenchymal Transition (EMT), which plays an important role in the progression of epithelial ovarian cancer. In this study, we analyzed the genotype and allele distribution as well as 3D modeling of one of the AKNA rs10817595 (−1372 C>A). The distribution of genotypes and alleles was analyzed using the T-ARMS PCR method on 63 ovarian cancer samples and 65 controls. AKNA mRNA expression was analyzed using qRT-PCR on 35 low-grade and 28 high-grade samples. Fifteen low-grade and 12 high-grade samples were analyzed for AKNA protein levels using immunohistochemistry. A 3D model of protein structure was constructed using AlphaFold. Significant differences in AKNA protein levels were found. However, no significant correlation was found for relative AKNA mRNA expression with protein levels. This result is thought to be related to decreased immune system response, increased inflammation, and increased EMT in epithelial ovarian cancer. AKNA gene variant (−1372 C>A) can cause a decrease in mRNA and protein levels in the low-grade and high-grade groups, so it has the potential as a genetic susceptibility factor in epithelial ovarian cancer.
Keywords: AKNA; genetic susceptibility; epithelial ovarian cancer AKNA; genetic susceptibility; epithelial ovarian cancer

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MDPI and ACS Style

Suryandari, D.A.; Miftahuzzakiyah, M.; Yunaini, L.; Kodariah, R.; Sukmawati, D.; Rustamadji, P.; Sari, P.; Ningsih, S.S. Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer. Curr. Issues Mol. Biol. 2025, 47, 78. https://doi.org/10.3390/cimb47020078

AMA Style

Suryandari DA, Miftahuzzakiyah M, Yunaini L, Kodariah R, Sukmawati D, Rustamadji P, Sari P, Ningsih SS. Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer. Current Issues in Molecular Biology. 2025; 47(2):78. https://doi.org/10.3390/cimb47020078

Chicago/Turabian Style

Suryandari, Dwi Anita, Miftahuzzakiyah Miftahuzzakiyah, Luluk Yunaini, Ria Kodariah, Dewi Sukmawati, Primariadewi Rustamadji, Puji Sari, and Sri Suciati Ningsih. 2025. "Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer" Current Issues in Molecular Biology 47, no. 2: 78. https://doi.org/10.3390/cimb47020078

APA Style

Suryandari, D. A., Miftahuzzakiyah, M., Yunaini, L., Kodariah, R., Sukmawati, D., Rustamadji, P., Sari, P., & Ningsih, S. S. (2025). Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer. Current Issues in Molecular Biology, 47(2), 78. https://doi.org/10.3390/cimb47020078

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