Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation
Abstract
Material and methods: The MIA PaCa-2 pancreatic carcinoma cell line was irradiated with 1.6- Gy proton beam. After irradiation, cell viability was measured colorimetrically, and the cell cycle, apoptosis, and gH2AX expression were evaluated on a FACScan cytometer.
Results: Low-dose proton beam irradiation had an effect on the MIA PaCa-2 tumor cell line already 1 h after exposure, but maximal lethality was reached after 72 h postirradiation with a cell viability rate of 24%. The cell cycle went into partial G1/0 arrest, and was released after 72 h. The expression of gH2AX was strong and its levels were significantly elevated as late as 48 h post radiation. The apoptosis levels increased with post radiation incubation time to reach 79% after 72 h.
Conclusions: Our data demonstrate that low-doses proton beam irradiation had an effect on MIA PaCa-2 pancreatic carcinoma cell line. Full extent of irradiation had an impact only 24 h postirradiation, triggering DNA arrested cell cycle in G1/0 phase. Formed DNA DSBs were found to be repaired via the NHEJ pathway mechanism within 72 h. Unsuccessful repaired DSBs induced apoptotic cell death. After 72 h reparation processes were completed, and cell cycle was released from arrest in G1/0 phase.
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Liubavičiūtė, A.; Kraśko, J.A.; Mlynska, A.; Lagzdina, J.; Sužiedėlis, K.; Pašukonienė, V. Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation. Medicina 2015, 51, 302-306. https://doi.org/10.1016/j.medici.2015.10.002
Liubavičiūtė A, Kraśko JA, Mlynska A, Lagzdina J, Sužiedėlis K, Pašukonienė V. Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation. Medicina. 2015; 51(5):302-306. https://doi.org/10.1016/j.medici.2015.10.002
Chicago/Turabian StyleLiubavičiūtė, Aušra, Jan Aleksander Kraśko, Agata Mlynska, Jelena Lagzdina, Kęstutis Sužiedėlis, and Vita Pašukonienė. 2015. "Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation" Medicina 51, no. 5: 302-306. https://doi.org/10.1016/j.medici.2015.10.002
APA StyleLiubavičiūtė, A., Kraśko, J. A., Mlynska, A., Lagzdina, J., Sužiedėlis, K., & Pašukonienė, V. (2015). Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation. Medicina, 51(5), 302-306. https://doi.org/10.1016/j.medici.2015.10.002
