Long Non-Coding RNA Expression in Laser Micro-Dissected Luminal A and Triple Negative Breast Cancer Tissue Samples—A Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients’ Characteristics and Tumor Samples
2.2. Laser Capture Microdissection (LCM)
2.3. RNA Extraction
2.4. LncRNA Profiling in FFPE Tissues
2.5. LncRNA Validation
2.6. Cell Lines
2.7. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Ethics Approval
Consent to Participate and for Publication
References
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Characteristics | TNBC N (%) | Luminal A N (%) | |
---|---|---|---|
Age | >50 | 7 (77.78) | 8 (88.89) |
<50 | 2 (22.22) | 1 (11.11) | |
BMI | Normal | 5 (55.56) | 5 (55.56) |
Obese | 4 (44.44) | 4 (44.44) | |
Stage | I and II | 5 (55.56) | 4 (44.44) |
III | 4 (44.44) | 5 (55.56) | |
Tumor size | <5 cm | 5 (55.56) | 6 (66.67) |
>5 cm | 4 (44.44) | 3 (33.33) | |
Lymph node involvement | Yes | 5 (55.56) | 4 (44.44) * |
No | 4 (44.44) | 3 (33.33) * | |
HER2 | Positive | 5 (55.56) | 5 (55.56) |
Negative | 4 (44.44) | 4 (44.44) | |
Ki-67 | Positive | 9 (100.00) | 9 (100.00) |
Negative | 0 (0.00) | 0 (0.00) | |
ER | Positive | 0 (0.00) | 9 (100.00) |
Negative | 9 (100.00) | 0 (0.00) | |
PR | Positive | 0 (0.00) | 9 (100.00) |
Negative | 9 (100.00) | 0 (0.00) |
LncRNA Name | Fold Change | p Value | LncRNA Name | Fold Change | p Value |
---|---|---|---|---|---|
BCYRN1 | 0.967 | 0.457 | MEG3 | 5.394 | 0.605 |
BDNF-AS | 0.894 | 0.469 | NEAT1 | 5.818 | 0.548 |
DISC2 | 0.624 | 0.325 | OTX2-AS1 | 205.222 | 0.029 |
EGOT | 1.634 | 0.860 | PANDAR | 1.101 | 0.236 |
FTX | 13.518 | 0.456 | PTENP1-AS | 0.989 | 0.708 |
GACAT1 | 1.347 | 0.728 | SNHG16 | 1.115 | 0.614 |
GAS5 | 1.734 | 0.914 | SOX2-OT | 0.020 | 0.042 |
H19 | 1.163 | 0.764 | ST7-AS2 | 2.206 | 0.956 |
HEIH | 1.388 | 0.702 | TERC | 1.652 | 0.812 |
HOTAIR | 5.771 | 0.543 | TMEM161B-AS1 | 0.909 | 0.474 |
IPW | 1.163 | 0.656 | TRERNA1 | 0.444 | 0.372 |
JPX | 1.014 | 0.512 | TUG1 | 1.453 | 0.814 |
KCNQ1OT1 | 4.028 | 0.690 | UCA1 | 0.386 | 0.601 |
FALEC | 1.204 | 0.633 | XIST | 0.769 | 0.316 |
LINC-ROR | 0.624 | 0.174 | ZFAS1 | 1.680 | 0.868 |
MALAT1 | 1.763 | 0.936 |
LncRNA | Sample Type | Fold Change | p Value |
---|---|---|---|
GAS5 | FFPE tissue | 0.33 | 0.022 |
Cell lines | 0.52 | 0.035 | |
NEAT1 | FFPE tissue | 0.66 | 0.27 |
Cell lines | 0.23 | 0.035 |
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Marcu, A.; Nitusca, D.; Vaduva, A.; Baderca, F.; Cireap, N.; Coricovac, D.; Dehelean, C.A.; Seclaman, E.; Ilina, R.; Marian, C. Long Non-Coding RNA Expression in Laser Micro-Dissected Luminal A and Triple Negative Breast Cancer Tissue Samples—A Pilot Study. Medicina 2021, 57, 371. https://doi.org/10.3390/medicina57040371
Marcu A, Nitusca D, Vaduva A, Baderca F, Cireap N, Coricovac D, Dehelean CA, Seclaman E, Ilina R, Marian C. Long Non-Coding RNA Expression in Laser Micro-Dissected Luminal A and Triple Negative Breast Cancer Tissue Samples—A Pilot Study. Medicina. 2021; 57(4):371. https://doi.org/10.3390/medicina57040371
Chicago/Turabian StyleMarcu, Anca, Diana Nitusca, Adrian Vaduva, Flavia Baderca, Natalia Cireap, Dorina Coricovac, Cristina Adriana Dehelean, Edward Seclaman, Razvan Ilina, and Catalin Marian. 2021. "Long Non-Coding RNA Expression in Laser Micro-Dissected Luminal A and Triple Negative Breast Cancer Tissue Samples—A Pilot Study" Medicina 57, no. 4: 371. https://doi.org/10.3390/medicina57040371