Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Ethics
2.2. Chemicals and Antibodies
2.3. Study Design
2.4. Measurement of Scratching Behaviour
2.5. Measurement of Ear Thickness
2.6. Measurement of Cytokine Levels
2.7. Histological Analysis
2.8. Immunohistochemical Analysis
2.9. Statistical Analysis
3. Results
3.1. Effect of Remote Ischemic Postconditioning on Ear Thickness and Itching Behaviour
3.2. Effect of Remote Ischemic Postconditioning on Tissue and Serum Cytokine Concentrations
3.3. Effect of Remote Ischemic Postconditioning on Histological Changes
3.3.1. Haematoxylin & Eosin Staining
3.3.2. Toluidine Staining
3.4. Effect of Remote Ischemic Postconditioning on T-Cell Infiltration
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gunduz, O.; Sapmaz-Metin, M.; Topuz, R.D.; Kaya, O.; Karadag, C.H.; Ulugol, A. Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis. Medicina 2023, 59, 1816. https://doi.org/10.3390/medicina59101816
Gunduz O, Sapmaz-Metin M, Topuz RD, Kaya O, Karadag CH, Ulugol A. Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis. Medicina. 2023; 59(10):1816. https://doi.org/10.3390/medicina59101816
Chicago/Turabian StyleGunduz, Ozgur, Melike Sapmaz-Metin, Ruhan Deniz Topuz, Oktay Kaya, Cetin Hakan Karadag, and Ahmet Ulugol. 2023. "Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis" Medicina 59, no. 10: 1816. https://doi.org/10.3390/medicina59101816