LINC00941 Promotes Cell Malignant Behavior and Is One of Five Costimulatory Molecule-Related lncRNAs That Predict Prognosis in Renal Clear Cell Carcinoma
Abstract
:1. Introduction
2. Methods
2.1. Data Collection
2.2. Identification of CMLs
2.3. CMLs Prognosis Analysis
2.4. Development of a Nomogram
2.5. Biological Enrichment
2.6. Immune Features and Immunotherapy Response Prediction
2.7. Cell Lines and qRT-Pcr Assay
2.8. Cell Transfection
2.9. Cell Proliferation Assay
2.10. Transwell Assays
2.11. Statistical Analysis
3. Results
3.1. Construction of CMLs Prognosis Signature
3.2. Validation and Evaluation of the Prognosis Model
3.3. Nomogram
3.4. Biological Enrichment
3.5. Immune Analysis
3.6. Exploring the Effect of LINC00941 in ccRCC
3.7. LINC00941 Promotes Cancer Cell Proliferation, Invasion, and Migration
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Features | Group | Number | Proportion (%) |
---|---|---|---|
Age | ≤60 | 262 | 51.0 |
>60 | 251 | 49.0 | |
Gender | Female | 176 | 34.3 |
Male | 337 | 65.7 | |
Grade | G1 | 12 | 2.4 |
G2 | 216 | 42.7 | |
G3 | 201 | 39.2 | |
G4 | 73 | 14.2 | |
unknown | 8 | 1.6 | |
Stage | Stage I | 255 | 49.8 |
Stage II | 56 | 10.9 | |
Stage III | 117 | 22.8 | |
Stage IV | 82 | 16.0 | |
unknown | 3 | 0.6 | |
T-classification | T1 | 261 | 50.8 |
T2 | 68 | 13.2 | |
T3 | 173 | 33.8 | |
T4 | 11 | 2.2 | |
N-classification | N0 | 229 | 44.7 |
N1 | 16 | 3.1 | |
unknown | 268 | 52.3 | |
M-classification | M0 | 407 | 79.3 |
M1 | 78 | 15.2 | |
unknown | 28 | 5.4 |
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Pan, H.; Wei, W.; Fu, G.; Pan, J.; Jin, B. LINC00941 Promotes Cell Malignant Behavior and Is One of Five Costimulatory Molecule-Related lncRNAs That Predict Prognosis in Renal Clear Cell Carcinoma. Medicina 2023, 59, 187. https://doi.org/10.3390/medicina59020187
Pan H, Wei W, Fu G, Pan J, Jin B. LINC00941 Promotes Cell Malignant Behavior and Is One of Five Costimulatory Molecule-Related lncRNAs That Predict Prognosis in Renal Clear Cell Carcinoma. Medicina. 2023; 59(2):187. https://doi.org/10.3390/medicina59020187
Chicago/Turabian StylePan, Huafeng, Wei Wei, Guanghou Fu, Jiaren Pan, and Baiye Jin. 2023. "LINC00941 Promotes Cell Malignant Behavior and Is One of Five Costimulatory Molecule-Related lncRNAs That Predict Prognosis in Renal Clear Cell Carcinoma" Medicina 59, no. 2: 187. https://doi.org/10.3390/medicina59020187