Infiltration by Intratumor and Stromal CD8 and CD68 in Cervical Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Immunohistochemical Examination
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patients Characteristics | N | % |
---|---|---|
FIGO 1 | 13 | 18.06 |
FIGO 2 | 12 | 16.66 |
FIGO 3 | 47 | 65.28 |
N0 | 25 | 34.72 |
N1 | 47 | 65.28 |
Total | 72 | 100 |
Primary Antibody | Clone | Company | Solutions | Visualization System | Platform | Cellular Specificity |
---|---|---|---|---|---|---|
CD8, Mo | C8/144B, Isotype: IgG1, kappa | Dako, Agilent, Glostrup, Denmark | Ready to use | EnVision™ FLEX, High pH, (Link), DAKO | AutostainerLink 48, DAKO | Cytotoxic T cells |
CD68, Mo | PG-M1, Isotype: IgG3, kappa | Dako, Agilent, Glostrup, Denmark | Ready to use | EnVision™ FLEX, High pH, (Link), DAKO | AutostainerLink 48, DAKO | Macrophages |
Primary Antibody | Control Tissue | Reaction Location in the Cells | Positive Quality Control |
---|---|---|---|
CD8 | Tonsil | Membrane and/or cytoplasm | Moderate to strong intensity of expression in T cells of the interfollicular areas; expression is absent in B cells and germinal centers |
CD68 | Tonsil | Cytoplasm | Moderate to strong intensity of expression in germinal center’s macrophages |
Stage Histologic Results | FIGO Stage I | FIGO Stage II | FIGO Stage III |
---|---|---|---|
Intratumoral CD8 | |||
missing | 0 | 1 | 2 |
low concentration | 6 | 7 | 38 |
high concentration | 7 | 4 | 7 |
Stromal CD8 | |||
missing | 0 | 0 | 1 |
low concentration | 7 | 7 | 25 |
high concentration | 6 | 5 | 21 |
Intratumoral CD68 | |||
missing | 0 | 0 | 1 |
low concentration | 7 | 9 | 37 |
high concentration | 6 | 3 | 9 |
Stromal CD68 | |||
missing | 0 | 0 | 0 |
low concentration | 11 | 6 | 25 |
high concentration | 2 | 6 | 22 |
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Dimitrova, P.; Vasileva-Slaveva, M.; Shivarov, V.; Hasan, I.; Yordanov, A. Infiltration by Intratumor and Stromal CD8 and CD68 in Cervical Cancer. Medicina 2023, 59, 728. https://doi.org/10.3390/medicina59040728
Dimitrova P, Vasileva-Slaveva M, Shivarov V, Hasan I, Yordanov A. Infiltration by Intratumor and Stromal CD8 and CD68 in Cervical Cancer. Medicina. 2023; 59(4):728. https://doi.org/10.3390/medicina59040728
Chicago/Turabian StyleDimitrova, Polina, Mariela Vasileva-Slaveva, Velizar Shivarov, Ihsan Hasan, and Angel Yordanov. 2023. "Infiltration by Intratumor and Stromal CD8 and CD68 in Cervical Cancer" Medicina 59, no. 4: 728. https://doi.org/10.3390/medicina59040728