Effects of Diabetes and Voluntary Exercise on IgA Concentration and Polymeric Immunoglobulin Receptor Expression in the Submandibular Gland of Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Sampling
2.3. Measurement of Blood Pressure and Heart Rate
2.4. Measurement of Blood Glucose Concentration
2.5. Measurement of Blood Albumin Concentration
2.6. Measurement of IgA Concentration
2.7. Measurement of Serum IgG Concentration
2.8. Measurement of Tyrosine Hydroxylase (TyrH) Concentration in SGs
2.9. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis
2.10. SG Tissue Preparation for Hematoxylin–Eosin Staining
2.11. Statistical Analysis
3. Results
3.1. Body Weight Gain and SG Weight
3.2. Blood Glucose Concentration, Mean Blood Pressure, Serum Albumin Concentration, and Heart Rate
3.3. Histological Changes in SGs due to Diabetes Mellitus and Voluntary Exercise
3.4. c-Tissue Weight, c-Digesta Weight, and IgA Concentrations in c-Digesta
3.5. IgA and IgG Concentrations in Serum
3.6. IgA Concentration, Poly-IgR mRNA Expression Level, and TyrH Concentration in SGs
3.7. Correlations of IgA Concentration in the SGs with Different Parameters
3.8. Correlation of Poly-IgR mRNA Expression Level in the SGs with Different Parameters
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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IgA Concentrations in SGs | |||
---|---|---|---|
r * | p | n | |
poly-IgR § mRNA expression level in SGs | 0.76 | 0.001 | 15 |
IgA concentration in serum | 0.68 | 0.005 | 15 |
Albumin concentration in serum | 0.63 | 0.01 | 15 |
IgG concentration in serum | 0.60 | 0.02 | 15 |
c-digesta ‡ weight | 0.16 | 0.6 | 15 |
IgA concentration in c-digesta | 0.056 | 0.8 | 15 |
Heart rate | −0.76 | 0.001 | 15 |
Mean blood pressure | −0.62 | 0.01 | 15 |
Blood glucose concentration | −0.59 | 0.02 | 15 |
c-tissue # weight | −0.44 | 0.1 | 15 |
TyrH † concentration in SGs | −0.11 | 0.7 | 15 |
Poly-IgR mRNA Expression Levels in SGs | |||
---|---|---|---|
r * | p | n | |
IgA concentration in serum | 0.81 | 0.0002 | 15 |
Albumin concentration in serum | 0.73 | 0.002 | 15 |
IgG concentration in serum | 0.64 | 0.01 | 15 |
IgA concentration in c-digesta | 0.094 | 0.7 | 15 |
c-digesta ‡ weight | 0.051 | 0.9 | 15 |
Heart rate | −0.75 | 0.001 | 15 |
Mean blood pressure | −0.64 | 0.01 | 15 |
Blood glucose concentration | −0.64 | 0.01 | 15 |
c-tissue # weight | −0.37 | 0.2 | 15 |
TyrH † concentration in SGs | −0.10 | 0.7 | 15 |
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Park, J.; Yamamoto, Y.; Hidaka, K.; Wada-Takahashi, S.; Takahashi, S.-s.; Morozumi, T.; Kubota, N.; Saita, M.; Saruta, J.; Sakaguchi, W.; et al. Effects of Diabetes and Voluntary Exercise on IgA Concentration and Polymeric Immunoglobulin Receptor Expression in the Submandibular Gland of Rats. Medicina 2023, 59, 789. https://doi.org/10.3390/medicina59040789
Park J, Yamamoto Y, Hidaka K, Wada-Takahashi S, Takahashi S-s, Morozumi T, Kubota N, Saita M, Saruta J, Sakaguchi W, et al. Effects of Diabetes and Voluntary Exercise on IgA Concentration and Polymeric Immunoglobulin Receptor Expression in the Submandibular Gland of Rats. Medicina. 2023; 59(4):789. https://doi.org/10.3390/medicina59040789
Chicago/Turabian StylePark, Jaebum, Yuko Yamamoto, Kouki Hidaka, Satoko Wada-Takahashi, Shun-suke Takahashi, Toshiya Morozumi, Nobuhisa Kubota, Makiko Saita, Juri Saruta, Wakako Sakaguchi, and et al. 2023. "Effects of Diabetes and Voluntary Exercise on IgA Concentration and Polymeric Immunoglobulin Receptor Expression in the Submandibular Gland of Rats" Medicina 59, no. 4: 789. https://doi.org/10.3390/medicina59040789