Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Exercise Protocol
2.3. Motor Nerve Conduction Velocity (MNCV)
2.4. Sciatic Nerve Sampling, Homogenization, and Protein Content Determination
2.5. Antioxidant Enzyme Activities
2.6. Western Blot Analysis
2.7. Data Analysis
3. Results
3.1. Blood Glucose Concentration
3.2. Body Weight
3.3. MNVC
3.4. MDA and 4HNE Contents
3.5. Antioxidant Enzyme Activities
3.6. NGF and BDNF Proteins
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cont (n = 10) | DM (n = 9) | DM + Ex (n = 8) | One-Way ANOVA | Effect Size d Value | ||
---|---|---|---|---|---|---|
p-Value | Con vs. DM | DM vs. DM + Ex | ||||
MNVC (m/s) | 45.1 ± 0.9 | 32.2 ± 1.1 *** | 36.0 ± 1.4 † | <0.001 | 4.25 | 1.06 |
Cont (n = 10) | DM (n = 9) | DM + Ex (n = 8) | One-Way ANOVA | Effect Size d Value | ||
---|---|---|---|---|---|---|
p-Value | Con vs. DM | DM vs. DM + Ex | ||||
Catalase (U/mg of protein) | 10.70 ± 0.93 | 8.20 ± 0.63 | 8.93 ± 0.82 | 0.092 | 1.00 | 0.35 |
GPx (mU/mg of protein) | 0.66 ± 0.07 | 0.86 ± 0.10 | 0.81 ± 0.09 | 0.235 | 0.76 | 0.17 |
SOD (U/mg of protein) | 1.01 ± 0.14 | 1.64 ± 0.22 * | 1.46 ± 0.09 | 0.029 | 1.12 | 0.36 |
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Nonaka, K.; Akiyama, J.; Une, S. Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats. Medicina 2024, 60, 480. https://doi.org/10.3390/medicina60030480
Nonaka K, Akiyama J, Une S. Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats. Medicina. 2024; 60(3):480. https://doi.org/10.3390/medicina60030480
Chicago/Turabian StyleNonaka, Koji, Junichi Akiyama, and Satsuki Une. 2024. "Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats" Medicina 60, no. 3: 480. https://doi.org/10.3390/medicina60030480