Do Stainless-Steel Pins Coated with Fibroblast Growth Factor–Calcium Phosphatase Composite Layers Have Anti-Infective Effects?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Screws Coated with Fibroblast Growth Factor–Calcium Phosphate Composite Layers
2.2. Scanning Electron Microscope of the Coating Layer
2.3. X-ray Diffraction of the Coating Layer
2.4. Coating Detaching Test
2.5. Sustained Release Test
2.6. Bioassay
2.7. Animal Experiments
2.8. Statistical Analysis
3. Results
3.1. Scanning Electron Microscope of the Coated Layer
3.2. X-ray Diffraction of the Coated Layer
3.3. Coating Detachment Test
3.4. Sustained Release Test
3.5. Bioassay
3.6. Animal Experiments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Infusion Solution Containing Calcium |
---|
Ringer’s solution, 500 mL (Otsuka, Otsuka Pharmaceutical Factory, Tokushima, Japan) |
Calcium chloride correction solution, 1 mEq/mL, 20 mL (Otsuka Pharmaceutical Factory, Tokushima, Japan) |
Intravenous solution containing phosphoric acid |
Klinisalz infusion solution, 500 mL (Kyowa CritiCare, Tokyo, Japan) |
Dipotassium phosphate injection, 20 mEq Kit, 20 mL (Terumo, Tokyo, Japan) |
Alkalizing agent (sodium bicarbonate solution) |
Meylon Intravenous Injection 7%, 20 mL (Otsuka Pharmaceutical Factory, Tokushima, Japan) |
Dissolving and diluting solution |
Otsuka Physiological Saline Injection, 20 mL (Otsuka Pharmaceutical Factory, Tokushima, Japan) |
Water for injection PL, 20 mL (Fuso, Fuso Pharmaceutical Industries, Osaka, Japan) |
FGF-2 (trafermin) |
Fiblast Spray 500 (Kaken Pharmaceuticals, Tokyo, Japan) |
Name of Soaking Solution | F0 | F4 |
---|---|---|
FGF-2 (μg/mL) | 0 | 4 |
Na+ (mM) | 138–139 | 138–139 |
K+ (mM) | 6.1–7.4 | 6.1–7.4 |
Ca2+ (mM) | 3.7–4.9 | 3.7–4.9 |
Mg2+ (mM) | 0.2 | 0.2 |
Cl− (mM) | 134–137 | 134–137 |
PO43− (mM) | 1.3–1.8 | 1.3–1.8 |
HCO3− (mM) | 15.1 | 15.1 |
Temperature (°C) | 37 | 37 |
Name | Substrate | Technique | Components of Coating Layer |
---|---|---|---|
Cp-FGF titanium method | Titanium | Immersed in F4 solution for 48 h | Calcium phosphate + FGF-2 |
Cp-FGF stainless-steel method | Stainless steel | Immersed in liquid F0 for 4 h, then immersed in liquid F4 for 44 h | Calcium phosphate + FGF-2 |
CP method | Stainless steel, titanium | Immersed in F0 solution for 48 h | calcium phosphate |
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© 2024 by the authors. Published by MDPI on behalf of the Lithuanian University of Health Sciences. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Totoki, Y.; Mutsuzaki, H.; Yanagisawa, Y.; Sogo, Y.; Yasunaga, M.; Noguchi, H.; Matsumoto, Y.; Koda, M.; Ito, A.; Yamazaki, M. Do Stainless-Steel Pins Coated with Fibroblast Growth Factor–Calcium Phosphatase Composite Layers Have Anti-Infective Effects? Medicina 2024, 60, 1419. https://doi.org/10.3390/medicina60091419
Totoki Y, Mutsuzaki H, Yanagisawa Y, Sogo Y, Yasunaga M, Noguchi H, Matsumoto Y, Koda M, Ito A, Yamazaki M. Do Stainless-Steel Pins Coated with Fibroblast Growth Factor–Calcium Phosphatase Composite Layers Have Anti-Infective Effects? Medicina. 2024; 60(9):1419. https://doi.org/10.3390/medicina60091419
Chicago/Turabian StyleTotoki, Yasukazu, Hirotaka Mutsuzaki, Yohei Yanagisawa, Yu Sogo, Mayu Yasunaga, Hiroshi Noguchi, Yukei Matsumoto, Masao Koda, Atsuo Ito, and Masashi Yamazaki. 2024. "Do Stainless-Steel Pins Coated with Fibroblast Growth Factor–Calcium Phosphatase Composite Layers Have Anti-Infective Effects?" Medicina 60, no. 9: 1419. https://doi.org/10.3390/medicina60091419