Diagnostic Roles of α-Methylacyl-CoA Racemase (AMACR) Immunohistochemistry in Gastric Dysplasia and Adenocarcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Tissue Array Methods
2.2. Immunohistochemical Staining
2.3. Evaluation of Immunohistochemistry
2.4. Statistical Analysis
3. Results
3.1. AMACR Expression in Gastric Dysplasia and Adenocarcinoma
3.2. Loss of AMACR Expression in Gastric Dysplasia and Adenocarcinoma
3.3. AMACR Expression in Normal Mucosa Adjacent to the Lesion
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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AMACR Negative | AMACR Positive | p-Value | ||
---|---|---|---|---|
Luminal and/or Cytoplasmic | Cytoplasmic Only | |||
Total (n = 79) | 36 (45.6) | 18 (22.8) | 25 (31.6) | |
Low-grade dysplasia (n = 20) | 4 (20.0) | 14 (70.0) | 2 (10.0) | <0.001 |
High-grade dysplasia (n = 19) | 9 (47.4) | 3 (15.8) | 7 (36.8) | |
Adenocarcinoma (n = 40) | 23 (57.5) | 1 (2.5) | 16 (40.0) |
AMACR Expression | p-Value | ||
---|---|---|---|
Positive | Negative | ||
Low-grade dysplasia (n = 20) | 16 (80.0) | 4 (20.0) | |
Age (years) | 64.69 ± 9.08 | 75.25 ± 5.56 | 0.041 |
Sex Male Female | 11 (68.8) 5 (31.3) | 4 (100.0) 0 (0.0) | 0.530 |
Tumor size (mm) | 16.56 ± 6.98 | 25.75 ± 5.12 | 0.025 |
High-grade dysplasia (n = 19) | 10 (52.6) | 9 (47.4) | |
Age (years) | 67.30 ± 8.04 | 69.00 ± 10.14 | 0.689 |
Sex Male Female | 6 (60.0) 4 (40.0) | 6 (66.7) 3 (33.3) | 1.000 |
Tumor size (mm) | 12.60 ± 7.75 | 8.89 ± 6.51 | 0.277 |
Adenocarcinoma (n = 40) | 17 (42.5) | 23 (57.5) | |
Age (years) | 69.00 ± 7.71 | 69.65 ± 8.79 | 0.808 |
Sex Male Female | 13 (69.8) 4 (30.2) | 20 (83.3) 3 (16.7) | 0.432 |
Tumor size (mm) | 28.59 ± 16.52 | 23.39 ± 11.89 | 0.254 |
AMACR Positivity | Discrepancy | ||
---|---|---|---|
Hotspot * | Overall | ||
Low-grade dysplasia (n = 20) | 20 (100.0) | 16 (80.0) | 4 (20.0) |
High-grade dysplasia (n = 19) | 12 (62.3) | 10 (52.6) | 2 (10.5) |
Adenocarcinoma (n = 40) | 26 (65.0) | 17 (42.5) | 9 (22.5) |
AMACR Expression in Lesion * | p-Value | ||
---|---|---|---|
Positive | Negative | ||
Normal mucosa adjacent to the lesion (n = 79) | |||
within 2 mm Positive Negative | 10 (23.3) 33 (76.7) | 9 (25.0) 27 (75.0) | 1.000 |
away 2 mm Positive Negative | 6 (14.0) 37 (86.0) | 8 (22.2) 28 (77.8) | 0.386 |
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Pyo, J.-S.; Min, K.-W.; Choi, J.-E.; Kang, D.-W. Diagnostic Roles of α-Methylacyl-CoA Racemase (AMACR) Immunohistochemistry in Gastric Dysplasia and Adenocarcinoma. Medicina 2024, 60, 1475. https://doi.org/10.3390/medicina60091475
Pyo J-S, Min K-W, Choi J-E, Kang D-W. Diagnostic Roles of α-Methylacyl-CoA Racemase (AMACR) Immunohistochemistry in Gastric Dysplasia and Adenocarcinoma. Medicina. 2024; 60(9):1475. https://doi.org/10.3390/medicina60091475
Chicago/Turabian StylePyo, Jung-Soo, Kyeung-Whan Min, Ji-Eun Choi, and Dong-Wook Kang. 2024. "Diagnostic Roles of α-Methylacyl-CoA Racemase (AMACR) Immunohistochemistry in Gastric Dysplasia and Adenocarcinoma" Medicina 60, no. 9: 1475. https://doi.org/10.3390/medicina60091475