In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Children’s Toothpaste Eluates
2.2. Isolation and Culture of Human Gingival Fibroblasts
2.3. MTT Assays
2.4. Cell Cycle Analysis
2.5. Cell Cytoskeleton Staining
2.6. Apoptosis/Necrosis Assays
2.7. ICP-MS and Ion Chromatography
2.8. Statistical Analysis
3. Results
3.1. MTT
3.2. Cell Cycle Analysis
3.3. Cell Cytoskeleton Staining
3.4. Apoptosis/Necrosis Assay
3.5. ICP-MS and Ion Chromatography
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Material | Manufacturer | Composition | Lot Number |
---|---|---|---|
Oral B Kids +3 | Procter & Gamble Poligono Industrial El Espartal, 1, 03100, Xixona, Alicante, Spain | Aqua, Hydrated Silica, Cocamidopropyl Betaine, Trisodium Phosphate, Aroma, Cellulose Gum, Sodium Fluoride, Carbomer, Sodium Saccharin, Limonene, Benzyl Alcohol, Sodium Benzoate, Cinnamal, Polysorbate 80, CÑ 42090 (1450 ppm Fluoride) | 0213028870 |
Fluor Kin Calcium | Laboratorios KIN SA Calle Fuerteventura, 4—PLT BJ, San Sebastian de los Reyes, Madrid, Spain | Aqua, Sorbitol, Glycerin, Hydrated Silica, Aroma, Titanium Dioxide, Xanthan Gum, Xylitol, Sodium Monofluorophosphate, Sodium Lauryl Sulfate, Sodium Methylparaben, Calcium Glycerophosphate, Citric Acid, Sodium Propylparaben, Potassium Acesulfame (1000 ppm Fluoride) | 20C09 |
PHB Junior | Laboratorios de Prevención e Higiene Bucal, Castanyer 25, 08022, Barcelona, Spain | Aqua, Sorbitol, Silica, Glycerin, Titanium Dioxide, Sodium Gluconate, Potassium Acesulfame, Sodium Lauryl Sulfate, PEG-40, Hydrogenated Castor Oil, Xanthan Gum, Sodium Fluoride, Sodium Saccharin, Sodium Methylparaben, Tocopheryl Acetate, Lactic Acid, Aroma (1000 ppm Fluoride) | M2005 |
Colgate 3+ | Colgate-Palmolive, C/Quintanavides, 19—Edificio 4. 28050, Madrid, Spain | Sorbitol, Aqua, Hydrated Silica, Xylitol, PEG-12, Cellulose Gum, Benzyl Alcohol, Sodium Lauryl Sulfate, Sodium Fluoride, Aroma (1000 ppm Fluoride) | (L)1007PL112C 1 |
Vitis Kids | Dentaid Benimaclet, 39, 46120 Alboraia, Valencia, Spain | Aqua, Sorbitol, Silica, Glycerin, Xylitol, Sodium Benzoate, Cellulose Gum, PEG-40, Hydrogenated Castor Oil, Sodium Lauryl Sarcosinate, Propylene Glycol, Sodium Fluoride, Sodium Saccharin, Benzoic Acid, Mica, Titanium Dioxide, Tetrasodium EDTA, Neohesperidin Dihydrochalcone, Aroma, CL16035 (1000 ppm Fluoride) | R2015 |
Ca (318.127 nm) ppm | K (766.491 nm) ppm | Mg (277.983 nm) ppm | Na (588.995 nm) ppm | P (214.914 nm) ppm | Si (251.432 nm) ppm | |
---|---|---|---|---|---|---|
Colgate | 1.1 | 1.1 | 0.6 | 459.5 | 1.4 | 12.1 |
Fluor Kin | 13.8 | 11 | 0.8 | 576.2 | 270.2 | 5.9 |
PHB Junior | 0 | 286.6 | 0 | 640.1 | 5.4 | 20.6 |
Oral B Kids | 0 | 2.3 | 0 | 1002.6 | 334.8 | 4.6 |
Vitis Kids | 0 | 1.2 | 0.1 | 843.1 | 2.5 | 10.7 |
Toothpaste | Fluoride Concentration |
---|---|
Colgate | 118.14 ± 2.22 mg/L |
Oral B Kids | 230.42 ± 5.56 mg/L |
Vitis Kids | 146.75 ± 5.08 mg/L |
PHB Junior | 163.54 ± 3.03 mg/L |
Fluor Kin | 22.91 ± 3.00 mg/L |
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Pecci-Lloret, M.P.; López-García, S.; Rodríguez-Lozano, F.J.; Álvarez-Novoa, P.; García-Bernal, D. In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts. Int. J. Environ. Res. Public Health 2022, 19, 2954. https://doi.org/10.3390/ijerph19052954
Pecci-Lloret MP, López-García S, Rodríguez-Lozano FJ, Álvarez-Novoa P, García-Bernal D. In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts. International Journal of Environmental Research and Public Health. 2022; 19(5):2954. https://doi.org/10.3390/ijerph19052954
Chicago/Turabian StylePecci-Lloret, María Pilar, Sergio López-García, Francisco Javier Rodríguez-Lozano, Pablo Álvarez-Novoa, and David García-Bernal. 2022. "In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts" International Journal of Environmental Research and Public Health 19, no. 5: 2954. https://doi.org/10.3390/ijerph19052954