Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Model and Exposure
2.2. Cigarette Smoke Exposure
2.3. Collection of Bronchoalveolar Lavage (BAL)
2.4. Total Cell Count in BAL Fluid
2.5. Differential Cell Count in BAL Fluid
2.6. Inflammatory Mediators
2.7. Measurement of Lung Mechanics
2.8. Lung Morphometry and Histopathology
2.9. Statistical Analysis
3. Results
3.1. Three Days (Acute) Exposure
3.1.1. Inflammatory Cellular Influx in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) and WT (Rhot1fl/fl) Mice
3.1.2. Effect of Acute, CS Exposure on Levels of Pro-Inflammatory Mediators in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) and WT (Rhot1fl/fl) Mice
3.2. Four Months (Chronic) Exposure
3.2.1. Inflammatory Cellular Influx in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) and WT (Rhot1fl/fl) Mice by Chronic CS Exposures
3.2.2. Effect of Chronic, CS Exposure on Levels of Pro-Inflammatory Mediators in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) and WT (Rhot1fl/fl) Mice
3.2.3. Effect of Chronic CS on Lung Mechanical Properties in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) WT (Rhot1fl/fl) Mice
3.2.4. Effect on Airspace Enlargement and Histopathology of Lung Tissues in Rhot1 Epithelial Cell Specific KO (Rhot1CreCC10) and WT (Rhot1fl/fl) Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sharma, S.; Wang, Q.; Muthumalage, T.; Rahman, I. Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke. Pathophysiology 2021, 28, 501-512. https://doi.org/10.3390/pathophysiology28040033
Sharma S, Wang Q, Muthumalage T, Rahman I. Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke. Pathophysiology. 2021; 28(4):501-512. https://doi.org/10.3390/pathophysiology28040033
Chicago/Turabian StyleSharma, Shikha, Qixin Wang, Thivanka Muthumalage, and Irfan Rahman. 2021. "Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke" Pathophysiology 28, no. 4: 501-512. https://doi.org/10.3390/pathophysiology28040033