Antimicrobial Activity of Hybrid Nanomaterials Based on Star and Linear Polymers of N,N′-Dimethylaminoethyl Methacrylate with In Situ Produced Silver Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of N,N′-Dimethylaminoethyl Methacrylate Star and Linear Polymers
2.3. In-Situ-Produced Silver Nanoparticles by N,N′-Dimethylaminoethyl Methacrylate Star and Linear Polymers
2.4. Quaternization of Linear and Star PDMAEMA in the Solution
2.5. Culture Media and Growth Conditions
2.5.1. Microorganisms
2.5.2. Preparation and Storage of Resazurin
2.5.3. Preparation of Standardized Inoculum
2.5.4. Preparation of 96 Well-Plates for Testing Reagents
2.6. Methods
3. Results and Discussion
3.1. Linear and Star poly(N,N′-Dimethylaminoethyl Methacrylate)s for AgNPs Preparation
3.2. Preparation and Characterization of the Hybrid Polymeric Materials with Incorporated AgNPs
3.3. Preparation of Quaternization of the Star and Linear PDMAEMA
3.4. Antimicrobial Activity of Obtained Materials
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Topology | DMAEMA:Initiator | Monomer Conversion [%] a | Mn [g/mol] b | Mw/Mn b |
---|---|---|---|---|---|
S-PDMAEMA | star | 2800:1 | 25 | 115,000 * | 2.6 |
L-PDMAEMA | linear | 1000:1 | 64 | 100,000 | 1.2 |
Time | AgNPs/S-PDMAEMA | AgNPs/L-PDMAEMA | ||
---|---|---|---|---|
Dh [nm] | Zeta Potential [mV] | Dh [nm] | Zeta Potential [mV] | |
1 h | 4.9; 38.8 | 38.0 | 2.4; 46.4 | 27.6 |
2 h | 4.8; 34.2 | 39.0 | 6.3; 46.8 | 27.5 |
3 h | 3.2; 38.9 | 39.6 | 4.1; 51.0 | 29.5 |
4 h | 4.7; 38.4 | 39.6 | 8.2; 58.9 | 31.2 |
5 h | 3.6; 33.7 | 39.4 | 5.6; 50.0 | 31.2 |
24 h | 4.2; 45.9 | 42.0 | 9.0; 64.7 | 35.3 |
48 h | 4.6; 42.3 | 42.8 | 8.9; 76.0 | 36.8 |
72 h | 4.9; 56.2 | 45.5 | 8.4; 76.3 | 39.4 |
96 h | 5.0; 69.5 | 49.8 | 8.4; 87.9 | 38.8 |
31 days | 8.4; 71.0 | 42.2 | 13.5; 92.0 | 39.2 |
Sample | STRAIN | ||||||||
---|---|---|---|---|---|---|---|---|---|
Pseudomonas aeruginosa 1390 | Escherichia coli W 1655 | Bacillus subtilis 168 | |||||||
MIC [mg/mL] | MBC [mg/mL] | MBC/MIC | MIC [mg/mL] | MBC [mg/mL] | MBC/MIC | MIC [mg/mL] | MBC [mg/mL] | MBC/MIC | |
S-PDMAEMA | 0.03 | 0.03 | 1 | 0.03 | 0.03 | 1 | 0.03 | 0.03 | 1 |
QS-PDMAEMA a | 0.03 | 0.03 | 1 | 0.03 | 0.03 | 1 | 0.015 | 0.015 | 1 |
AgNPs/S-PDMAEMA b | 0.01 | 0.01 | 1 | 0.01 | 0.01 | 1 | 0.01 | 0.01 | 1 |
L-PDMAEMA | 0.125 | 0.125 | 1 | 0.125 | 0.125 | 1 | 0.03 | 0.03 | 1 |
QL-PDMAEMA c | 0.06 | 0.06 | 1 | 0.06 | 0.06 | 1 | 0.03 | 0.03 | 1 |
AgNPs/L-PDMAEMA d | 0.01 | 0.01 | 1 | 0.02 | 0.02 | 1 | 0.01 | 0.01 | 1 |
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Teper, P.; Sotirova, A.; Mitova, V.; Oleszko-Torbus, N.; Utrata-Wesołek, A.; Koseva, N.; Kowalczuk, A.; Mendrek, B. Antimicrobial Activity of Hybrid Nanomaterials Based on Star and Linear Polymers of N,N′-Dimethylaminoethyl Methacrylate with In Situ Produced Silver Nanoparticles. Materials 2020, 13, 3037. https://doi.org/10.3390/ma13133037
Teper P, Sotirova A, Mitova V, Oleszko-Torbus N, Utrata-Wesołek A, Koseva N, Kowalczuk A, Mendrek B. Antimicrobial Activity of Hybrid Nanomaterials Based on Star and Linear Polymers of N,N′-Dimethylaminoethyl Methacrylate with In Situ Produced Silver Nanoparticles. Materials. 2020; 13(13):3037. https://doi.org/10.3390/ma13133037
Chicago/Turabian StyleTeper, Paulina, Anna Sotirova, Violeta Mitova, Natalia Oleszko-Torbus, Alicja Utrata-Wesołek, Neli Koseva, Agnieszka Kowalczuk, and Barbara Mendrek. 2020. "Antimicrobial Activity of Hybrid Nanomaterials Based on Star and Linear Polymers of N,N′-Dimethylaminoethyl Methacrylate with In Situ Produced Silver Nanoparticles" Materials 13, no. 13: 3037. https://doi.org/10.3390/ma13133037