Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Gingival Mesenchymal Stromal Cell (gMSC) Isolation and Characterization
2.3. Preparation of Experimental Culture Media
2.4. Cell Proliferation (CCK-8)
2.5. Cell Survival by MTT Assay
2.6. Cell Culture on Treated Root Samples
2.7. Statistical Analysis
3. Results
3.1. CCK Analysis
3.2. MTT Analysis
3.3. SEM Observations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Product | Manufacturer | Composition | Indication |
---|---|---|---|
HYBENX® [35,36] (HI) | Epien Medical, St Paul, MN, USA | Hygroscopic solution Sulfonated phenolics (60%) Sulfuric acid (28%) Water (12%) | Oral Tissue Decontaminant Removal of plaque biofilm Topical irrigation during scaling and root planing Topical rinse/solution for oral cavity Adjunctive rinse of tooth root canal systems in endodontic treatments |
Perisolv® [29] (PS) | Regedent AG, Zurich, Switzerland | Gel: Amino acids (glutamic acid, leucine, lysine) Sodium chloride, Sodium carboxymethyl cellulose Ultrapure water Liquid: Sodium hypochlorite solution 0.95% | Adjunctive therapy to scaling and root planing in periodontitis and peri-implantitis due to inhibitory bacterial effects and biofilm elimination |
Perioflush® [37] (PF) | Dental Life Sciences LTD, Wigan, UK | Water based solution: Ultrapurified water Silver nanocolloid solution Sodium nitrate Apple, mint flavours Lactic acid Phosphoric acid | Rinsing and cleaning the gums and oral mucosa Rinsing around bridge spans, erupting teeth, around implant areas, overhanging fillings, interdental spaces, after scaling |
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Lupșe, I.; Pall, E.; Barbu Tudoran, L.; Bulboacă, A.E.; Ciurea, A.; Micu, I.C.; Roman, A.; Delean, A.G.; Muntean, A.; Soancă, A. Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment. Materials 2021, 14, 5049. https://doi.org/10.3390/ma14175049
Lupșe I, Pall E, Barbu Tudoran L, Bulboacă AE, Ciurea A, Micu IC, Roman A, Delean AG, Muntean A, Soancă A. Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment. Materials. 2021; 14(17):5049. https://doi.org/10.3390/ma14175049
Chicago/Turabian StyleLupșe, Irina, Emoke Pall, Lucian Barbu Tudoran, Adriana Elena Bulboacă, Andreea Ciurea, Iulia Cristina Micu, Alexandra Roman, Ada Gabriela Delean, Alexandrina Muntean, and Andrada Soancă. 2021. "Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment" Materials 14, no. 17: 5049. https://doi.org/10.3390/ma14175049