Biosynthesis and Response of Zinc Oxide Nanoparticles against Periimplantitis Triggering Pathogens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. APLAE Preparation
2.3. ZnPs Biosynthesis
2.4. Optimization of ZnPs Biosynthesis
2.5. Stability of ZnPs
2.6. Antimicrobial Activity of ZnPs
2.7. Characterization of ZnPs
3. Results
3.1. Biosynthesis of ZnPs
3.2. Optimization of ZnPs Biosynthesis Parameters
3.2.1. Optimization of Zinc Acetate Concentration
3.2.2. Optimization of APLAE Volume
3.2.3. Optimization of pH
3.2.4. Optimization of Temperature
3.3. Stability of ZnPs
3.4. Characterization of ZnPs
3.4.1. Fourier Transformed Infrared (FTIR) Analysis
3.4.2. Field Emission Scanning Electron Microscopy (FESEM)
3.4.3. X-ray Diffraction (XRD) Analysis
3.4.4. Energy Dispersive X-ray Diffraction (EDX) Analysis
3.5. Antimicrobial Activity of ZnPs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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2θ | hkl | FWHM (β) | D (nm) |
---|---|---|---|
31.70 | 100 | 0.9890 | 87.18 |
34.33 | 002 | 0.9700 | 88.89 |
36.17 | 101 | 0.9971 | 86.17 |
47.45 | 102 | 0.9163 | 98.14 |
56.52 | 110 | 0.9515 | 98.97 |
62.78 | 103 | 0.9859 | 98.56 |
69.02 | 201 | 0.9850 | 98.65 |
Microorganism | Zone of Inhibition in mm | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ZnPs | APLAE | Ciprofloxacin | |||||||||||
Concentration (mg/mL) | 1.0 | 2.0 | 4.0 | 6.0 | 8.0 | 10.0 | 1.0 | 2.0 | 4.0 | 6.0 | 8.0 | 10.0 | 10 µg/mL |
S. aureus | 16 ± 1 | 18 ± 1 | 18.5 ± 0.5 | 19 ± 2 | 19 ± 3 | 25 ± 1 | N/A | N/A | N/A | N/A | N/A | N/A | 21 ± 0.58 |
E. coli | 8 ± 1 | 10 ± 0.57 | 15 ± 0.57 | 19 ± 1 | 20.5 ± 1.5 | 22 ± 0.57 | N/A | N/A | N/A | N/A | 15.5 ± 1.5 | 16 ± 0.57 | 20 ± 0.00 |
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Ting, B.Y.S.; Fuloria, N.K.; Subrimanyan, V.; Bajaj, S.; Chinni, S.V.; Reddy, L.V.; Sathasivam, K.V.; Karupiah, S.; Malviya, R.; Meenakshi, D.U.; et al. Biosynthesis and Response of Zinc Oxide Nanoparticles against Periimplantitis Triggering Pathogens. Materials 2022, 15, 3170. https://doi.org/10.3390/ma15093170
Ting BYS, Fuloria NK, Subrimanyan V, Bajaj S, Chinni SV, Reddy LV, Sathasivam KV, Karupiah S, Malviya R, Meenakshi DU, et al. Biosynthesis and Response of Zinc Oxide Nanoparticles against Periimplantitis Triggering Pathogens. Materials. 2022; 15(9):3170. https://doi.org/10.3390/ma15093170
Chicago/Turabian StyleTing, Bernice Yii Shu, Neeraj Kumar Fuloria, Vetriselvan Subrimanyan, Sakshi Bajaj, Suresh V. Chinni, Lebaka Veeranjaneya Reddy, Kathiresan V. Sathasivam, Sundram Karupiah, Rishabha Malviya, Dhanalekshmi Unnikrishnan Meenakshi, and et al. 2022. "Biosynthesis and Response of Zinc Oxide Nanoparticles against Periimplantitis Triggering Pathogens" Materials 15, no. 9: 3170. https://doi.org/10.3390/ma15093170