Potential of Crude Extract of Streptomyces sp. nov., Strain TRM76147 for Control of A. gaisen
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains TRM76147 and A. gaisen Were Obtained
2.2. 16S rRNA Gene and Multilocus Sequence Analysis (MLSA) Phylogeny
2.3. Polyphasic Isolation and Characterization of Strain TRM76147
2.3.1. Culture Characteristics of TRM76147
2.3.2. Peptidoglycan, Amino Acids, and Polar Lipids of TRM76147
2.4. Genomic Metabolic Potential Analysis of TRM76147
2.5. Stability Analysis of Active Crude Extract of Strain TRM76147
2.6. TRM76147 Exploration of the Mechanism of Inhibition of A. gaisen by Crude Extracts and Assessment of Bioprophylactic Potential of Pears
2.6.1. Investigation of the Mechanism of Inhibition of A. gaisen by the Crude Extract of TRM76147
2.6.2. Evaluation for Plant Pathogen Suppression Ability
2.7. Statistical Analysis
3. Results
3.1. Genetic Analysis of Strain TRM76147
3.2. Growth and Cultural Characteristics of Strain TRM76147 Compared with Reference Strains
Characterization of the Chemical Components of Bacterial Cell Walls
3.3. Analysis of Secondary Metabolites in TRM76147 Strain Using antiSMASH and bigSCAPE Software
3.4. Strain 76147 Antimicrobial Activity Analysis
3.4.1. Investigation of Fungistatic Effects of Streptomyces TRM76147 on A. gaisen Using Stepwise Extraction Method
3.4.2. Impact of Temperature on Antifungal Efficacy of TRM76147 Fermentation Crude Extract
3.4.3. Stability and Activity of TRM76147 Extract under UV and pH Conditions
3.5. Effect of Crude Extract of Strain 76147 on the Activity of A. gaisen
3.5.1. Impact of TRM76147 Compounds on A. gaisen Mycelium and Conidium Integrity
3.5.2. Impact of TRM76147 Fermentation Broth on A. gaisen Growth
4. Discussions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Feature | TRM76147 | Streptomyces griseoviridis NBRC12874T | Streptomyces niveoruber NBRC15428T |
---|---|---|---|
Color of aerial hyphae | White | Pale greyish-purple | Ivory |
Matrix hyphal color | Yellow | Purplish-red | Pale yellow |
Spore chain morphology | Straight | Rectiflexibiles | Straight |
Pigment production | Yellow | None | Brown |
Temperature range (°C) | 20–45 | ≤45 | <45 |
pH range | 7–10 | 5–12 | 6–11 |
NaCl range (%) | ≤5 | ≤7 | <7 |
Sucrose | + | ND | ND |
Maltose | + | + | + |
L-arabinose | + | (+) | + |
Galactose | + | ND | ND |
Lactose | ND | + | (+) |
Inositol | + | + | ND |
Dextrin | + | + | ND |
Melanin production | − | − | − |
Hydrogen sulfide production | + | − | − |
Gelatin liquefaction | + | + | + |
Protease production | + | + | − |
Amylase production | + | ND | ND |
Nitrate reduction | + | ND | ND |
Oxidase production | + | ND | ND |
Production of catalase | + | ND | ND |
Urease production | − | ND | ND |
Tween 20 | + | + | + |
Cellulose decomposition | + | ND | ND |
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Chen, Y.-H.; Zhang, J.-X.; Yang, G.; Liu, Y.; Ran, S.; Wang, J.-M.; Liu, Q.; Luo, X.-X. Potential of Crude Extract of Streptomyces sp. nov., Strain TRM76147 for Control of A. gaisen. Forests 2024, 15, 1605. https://doi.org/10.3390/f15091605
Chen Y-H, Zhang J-X, Yang G, Liu Y, Ran S, Wang J-M, Liu Q, Luo X-X. Potential of Crude Extract of Streptomyces sp. nov., Strain TRM76147 for Control of A. gaisen. Forests. 2024; 15(9):1605. https://doi.org/10.3390/f15091605
Chicago/Turabian StyleChen, Yi-Huang, Jia-Xin Zhang, Guo Yang, Yang Liu, Song Ran, Jian-Ming Wang, Qin Liu, and Xiao-Xia Luo. 2024. "Potential of Crude Extract of Streptomyces sp. nov., Strain TRM76147 for Control of A. gaisen" Forests 15, no. 9: 1605. https://doi.org/10.3390/f15091605