Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Flow Cytometry
2.3. Immunofluorescence Imaging (IF)
2.4. Gold Nanorods (AuNRs)
2.5. Cytotoxicity Assay
2.6. Quantification of AuNR Targeting
2.7. Photothermal Therapy
2.8. Apoptosis and Necrosis
3. Results
3.1. EGFR Is Highly Expressed in HNSCC Cell Lines
3.2. AuNRs Cytotoxicity
3.3. In Vitro Targeting Efficiency
3.4. NIR-II Photothermal Therapy (PTT) Significantly Increased Cancer Cell Death in Head and Neck Cancer
3.5. Combination of tAuNRs and Laser Therapy Caused Cell Death by Apoptosis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Egnuni, T.; Ingram, N.; Mirza, I.; Coletta, P.L.; McLaughlan, J.R. Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window. Pharmaceutics 2021, 13, 1651. https://doi.org/10.3390/pharmaceutics13101651
Egnuni T, Ingram N, Mirza I, Coletta PL, McLaughlan JR. Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window. Pharmaceutics. 2021; 13(10):1651. https://doi.org/10.3390/pharmaceutics13101651
Chicago/Turabian StyleEgnuni, Teklu, Nicola Ingram, Ibrahim Mirza, P. Louise Coletta, and James R. McLaughlan. 2021. "Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window" Pharmaceutics 13, no. 10: 1651. https://doi.org/10.3390/pharmaceutics13101651