Potential Wound Healing and Anti-Melanogenic Activities in Skin Cells of Aralia elata (Miq.) Seem. Flower Essential Oil and Its Chemical Composition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Extraction of Aralia elata (Miq.) Seem. Flower Absolute-Type Essential Oil
2.3. Compounds Analysis in Aralia elata (Miq.) Seem. Flower Absolute-Type Essential Oil
2.4. Cell Culture
2.5. Cell Viability Assay
2.6. Proliferation Assay
2.7. Cell Migration Assay
2.8. Collagen Synthesis Assay
2.9. Western Blotting
2.10. Melanin Content Assay
2.11. Tyrosinase Activity Analysis
2.12. Analysis
3. Results and Discussion
3.1. Proliferation and Migration of AESFEO-Treated HaCaT Cells
3.2. Influence of AESFEO on Collagen Synthesis in HaCaT Cells
3.3. AESFEO Induced Changes in Intracellular Signal Kinases in HaCaT Cells
3.4. Cell Proliferation, Melanin Synthesis, and Tyrosinase Activity in AESFEO-Treated B16BL6 Cells
3.5. Effect of AESFEO on Melanogenesis Regulation-Related Proteins in B16BL6 Cells
3.6. MAPK Phosphorylations in AESFEO-Treated B16BL6 Cells
3.7. Chemical Composition of AESFEO
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No | Component Name | RT 1 | RI 2 | Area (%) | CAS No. | |
---|---|---|---|---|---|---|
Observed | Literature | |||||
1 | l-Bornyl acetate | 33.00 | 1280 | 1280 | 1.37 | 5655-61-8 |
2 | Caryophyllene | 41.36 | 1410 | 1418 | 1.42 | 87-44-5 |
3 | α-Bisabolene | 47.06 | 1503 | 1547 | 1.78 | 25532-79-0 |
4 | β-Bisabolene | 51.86 | 1585 | 1509 | 1.01 | 495-61-4 |
5 | Farnesol | 58.95 | 1713 | 1713 | 1.60 | 4602-84-0 |
6 | Hexadecanoic acid | 71.84 | 1968 | 1984 | 2.87 | 57-10-3 |
7 | Linolenic acid | 79.83 | 2142 | 2199 | 11.85 | 463-40-1 |
8 | Antioxidant BKF | 90.47 | 2394 | 2398 | 7.51 | 119-47-1 |
9 | 1-Octadecene | 94.25 | 2491 | 1800 | 0.98 | 112-88-9 |
10 | Pentacosane | 94.60 | 2500 | 2500 | 1.48 | 629-99-2 |
11 | Cyclotetracosane | 100.34 | 2697 | 2445 | 14.31 | 297-03-0 |
12 | Muscalure | 102.46 | 2798 | 2272 | 2.28 | 27519-02-4 |
13 | 1-Docosene | 104.34 | 2902 | 2196 | 14.00 | 1599-67-3 |
14 | δ-Tocopherol | 104.83 | 2932 | 2951 | 3.40 | 119-13-1 |
15 | γ-Tocopherol | 106.52 | 3040 | 3055 | 26.93 | 7616-22-0 |
16 | 9-(β-Chlorostyryl)-10-(phenylethynyl)anthracene | 107.11 | 3080 | - | 1.05 | 80364-59-6 |
17 | α-Amyrin | 110.68 | 3330 | 3376 | 3.17 | 638-95-9 |
18 | dl-Tetramisole | 114.94 | 3556 | - | 2.99 | 5036-02-2 |
Total Identified (%) | 100.00 |
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Kim, D.Y.; Won, K.J.; Kim, Y.Y.; Yoo, D.Y.; Lee, H.M. Potential Wound Healing and Anti-Melanogenic Activities in Skin Cells of Aralia elata (Miq.) Seem. Flower Essential Oil and Its Chemical Composition. Pharmaceutics 2024, 16, 1008. https://doi.org/10.3390/pharmaceutics16081008
Kim DY, Won KJ, Kim YY, Yoo DY, Lee HM. Potential Wound Healing and Anti-Melanogenic Activities in Skin Cells of Aralia elata (Miq.) Seem. Flower Essential Oil and Its Chemical Composition. Pharmaceutics. 2024; 16(8):1008. https://doi.org/10.3390/pharmaceutics16081008
Chicago/Turabian StyleKim, Do Yoon, Kyung Jong Won, Yoon Yi Kim, Da Yeon Yoo, and Hwan Myung Lee. 2024. "Potential Wound Healing and Anti-Melanogenic Activities in Skin Cells of Aralia elata (Miq.) Seem. Flower Essential Oil and Its Chemical Composition" Pharmaceutics 16, no. 8: 1008. https://doi.org/10.3390/pharmaceutics16081008