Au@CuS Nanoshells for Surface-Enhanced Raman Scattering Image-Guided Tumor Photothermal Therapy with Accelerated Hepatobiliary Excretion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cells and Animals
2.3. Preparation of Nanoparticles
2.3.1. Preparation of PEG-AuNPs or PEG-Au-DTTC NPs
2.3.2. Preparation of PEG-Au@CuS NSs or PEG-Au-DTTC@CuS NSs
2.4. Characterization of Different Nanoparticles
2.5. Raman Stability in FBS
2.6. In Vitro Cytotoxicity and Photothermal Ablation Effect of PEG-Au-DTTC@CuS NSs
2.7. Isolation of Primary Mouse Hepatocytes and Kupffer Cells
2.8. Quantification of Au in Mouse Hepatocytes and Kupffer Cells Following Incubation with Different Nanoparticles
2.9. Excretion of Nanoparticles into Gallbladders of Mice
2.10. In Vitro Photothermal Conversion Effect
2.11. Establishment of Tumor Models
2.12. Raman Measurements
2.13. Raman Image-Guided Tumor Photothermal Therapy
2.14. Statistical Analysis
3. Results
3.1. Characterization of PEG-AuNPs and PEG-Au@CuS NSs
3.2. Hepatobiliary Excretion of PEG-AuNPs and PEG-Au@CuS NSs
3.3. UV Absorption and In Vitro Photothermal Effect
3.4. Raman Spectra of the DTTC-Loaded Nanoparticles
3.5. In Vivo Photothermal Effect
3.6. In Vivo Raman Imaging
3.7. Therapeutic Efficacy of Intraoperative Raman Image-Guided PTT by PEG-Au-DTTC@CuS NSs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, S.; Yu, S.; Sun, J.; Huang, T.; Lin, H.; Li, Z.; Xiao, Z.; Lu, W. Au@CuS Nanoshells for Surface-Enhanced Raman Scattering Image-Guided Tumor Photothermal Therapy with Accelerated Hepatobiliary Excretion. Pharmaceutics 2024, 16, 1089. https://doi.org/10.3390/pharmaceutics16081089
Zhang S, Yu S, Sun J, Huang T, Lin H, Li Z, Xiao Z, Lu W. Au@CuS Nanoshells for Surface-Enhanced Raman Scattering Image-Guided Tumor Photothermal Therapy with Accelerated Hepatobiliary Excretion. Pharmaceutics. 2024; 16(8):1089. https://doi.org/10.3390/pharmaceutics16081089
Chicago/Turabian StyleZhang, Sihang, Sheng Yu, Jingwen Sun, Teng Huang, Hongzheng Lin, Zhe Li, Zeyu Xiao, and Wei Lu. 2024. "Au@CuS Nanoshells for Surface-Enhanced Raman Scattering Image-Guided Tumor Photothermal Therapy with Accelerated Hepatobiliary Excretion" Pharmaceutics 16, no. 8: 1089. https://doi.org/10.3390/pharmaceutics16081089