Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Soil Samples
2.2. Isolation, Screening, and Identification of Bacterial Strains
2.3. Assay for the Biosurfactant Production (Drop Collapse Assay)
2.4. Crude Oil Degradation
2.5. Biosurfactant Extraction
2.6. Biosurfactant Purification and Calculation of Critical Micelle Concentration
2.7. Biosurfactant Characterization
2.8. Emulsification Assay
2.9. Determination of Chemical Oxygen Demand Reduction
2.10. Microbial Fuel Cell Design, Inoculation, and Electricity Generation
3. Results
3.1. Screening, Selection and Identification of Biosurfactant-Producing Bacteria
3.2. Characterization of Hydrocarbon-Degrading and Biosurfactant-Producing Bacteria
Biochemical Test | B1 (B. subtilis) | B2 (P. aeruginosa) |
---|---|---|
Citrate utilization | + | + |
Lysine utilization | − | − |
Ornithine utilization | − | − |
Urease | − | − |
Phenylalanine deamination | − | − |
Nitrate reduction | + | + |
H2S production | − | − |
Glucose | + | − |
Adonitol | − | − |
Lactose | − | − |
Arabinose | + | + |
Sorbitol | + | − |
3.3. Biodegradation of Crude Oil by Isolated Bacteria
3.4. Extraction and CMC Determination of Crude Biosurfactant
3.5. Biosurfactant Characterization
3.6. Emulsification Assay
3.7. Determination of COD Reduction
3.8. Application of Hydrocarbon-Degrading Bacteria in Bioremediation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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PAHs | Crude Oil (Abiotic Control) | Crude Oil Treated by Strain B1 for 4 Week | Crude Oil Treated by Strain B2 for 4 Week | Percentage of Degradation (%) by Strain B1 | Percentage of Degradation (%) by Strain B2 |
---|---|---|---|---|---|
Anthracene | Available (1.51) | Available (0.46) | Available (0.63) | 70.05 | 58.2 |
Fluorene | Available (1.63) | Available (0.39) | Available (0.50) | 76.80 | 68.90 |
1H-Indene | Available (0.11) | Not available | Available (0.11) | 100 | 00.00 |
Naphthalene | Available (5.24) | Available (1.23) | Available (1.29) | 76.50 | 75.20 |
Phenanthrene | Available (1.25) | Available (0.34) | Available (1.25) | 72.30 | 74.80 |
Benzene | Available (2.61) | Available (1.53) | Available (0.58) | 58.70 | 77.4 |
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Sharma, K.; Singh, V.; Pandit, S.; Thapa, B.S.; Pant, K.; Tusher, T.R. Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation. Sustainability 2022, 14, 15638. https://doi.org/10.3390/su142315638
Sharma K, Singh V, Pandit S, Thapa BS, Pant K, Tusher TR. Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation. Sustainability. 2022; 14(23):15638. https://doi.org/10.3390/su142315638
Chicago/Turabian StyleSharma, Kalpana, Vandana Singh, Soumya Pandit, Bhim Sen Thapa, Kumud Pant, and Tanmoy Roy Tusher. 2022. "Isolation of Biosurfactant-Producing Bacteria and Their Co-Culture Application in Microbial Fuel Cell for Simultaneous Hydrocarbon Degradation and Power Generation" Sustainability 14, no. 23: 15638. https://doi.org/10.3390/su142315638