Screening on the Presence of Plant Growth Regulators in High Biomass Forming Seaweeds from the Ionian Sea (Mediterranean Sea)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Algae
2.2. Preparation of Standard Solutions
2.3. Sample Preparation
2.4. HPLC Set-Up
2.5. Method Validation
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Voucher ID | |
---|---|---|
Rhodophyta | Hypnea corona Huisman et Petrocelli | PhL705 |
Spyridia filamentosa (Wulfen) Harvey | PhL706 | |
Chlorophyta | Chaetomorpha linum (O.F. Müller) Kützing | PhL707 |
Ulvalacinulata (Kützing) Wittrock | PhL708 |
Analyte | Range (µg/mL) | Equations | R2 | LOD (µg/mL) | Degree of Freedom |
---|---|---|---|---|---|
GA3 | 10–1000 | y = 0.0433x + 0.0013 | 0.9896 | 5 | 6 |
KN | 0.1–10 | y = 3.9094x − 0.0008 | 0.9937 | 0.2 | 6 |
IAA | 0.1–10 | y = 0.5829x + 0.0006 | 0.9967 | 0.2 | 6 |
ABA | 0.1–10 | y = 1.8133x − 0.0004 | 0.9985 | 0.2 | 6 |
IBA | 0.1–10 | y = 0.4685x + 0.0007 | 0.9965 | 1.8 | 6 |
Species | GA3 (µg/mL) | KN (µg/mL) | IAA (µg/mL) | ABA (µg/mL) | IBA (µg/mL) |
---|---|---|---|---|---|
Hypneacorona Huisman et Petrocelli | 1038.00 ± 2.00 | 0.57 ± 0.07 | 6.70 ± 0.30 | 1.10 ± 0.40 | LOQ |
Spryridia filamentosa (Wulfen) Harvey | 6.30 ± 0.10 | 1.70 ± 0.20 | 63.60 ± 0.50 | 8.40 ± 0.90 | 17.90 ± 0.00 |
Chaetomorpha linum (O.F. Müller) Kützing | 5.40 ± 0.40 | 0.31 ± 0.05 | LOD | 0 | LOQ |
Ulvalacinulata (Kützing) Wittrock | 0 | 0.48 ± 0.06 | 2.30 ± 0.10 | 0.72 ± 0.02 | LOQ |
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Spagnuolo, D.; Russo, V.; Manghisi, A.; Di Martino, A.; Morabito, M.; Genovese, G.; Trifilò, P. Screening on the Presence of Plant Growth Regulators in High Biomass Forming Seaweeds from the Ionian Sea (Mediterranean Sea). Sustainability 2022, 14, 3914. https://doi.org/10.3390/su14073914
Spagnuolo D, Russo V, Manghisi A, Di Martino A, Morabito M, Genovese G, Trifilò P. Screening on the Presence of Plant Growth Regulators in High Biomass Forming Seaweeds from the Ionian Sea (Mediterranean Sea). Sustainability. 2022; 14(7):3914. https://doi.org/10.3390/su14073914
Chicago/Turabian StyleSpagnuolo, Damiano, Valentino Russo, Antonio Manghisi, Antonio Di Martino, Marina Morabito, Giuseppa Genovese, and Patrizia Trifilò. 2022. "Screening on the Presence of Plant Growth Regulators in High Biomass Forming Seaweeds from the Ionian Sea (Mediterranean Sea)" Sustainability 14, no. 7: 3914. https://doi.org/10.3390/su14073914