Effects of Replacing Fishmeal with Algal Biomass (Pavlova sp. 459) on Membrane Lipid Composition of Atlantic Salmon (Salmo salar) Parr Muscle and Liver Tissues

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

Pls see attached file.

Comments for author File: Comments.pdf

Author Response

Thank you for your feedback. Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

1- Add Abbreviations in tables.

2- Some figures (pictures) have overlapping words and numbers

3- Add new references and remove the oldest.

 

Comments for author File: Comments.pdf

Author Response

Thank you for your feedback, please see the attachment.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

1. The title does not match the topic of the special issue. Therefore, it is not suitable for the publication of this special issue. It is advisable to find a suitable journal for publication.

2. The experiment was well executed. The article is also well written, including results and discussions.

3. Lack of explanation of research structure, design, and experimental hypotheses.

4. Line 300-306, this paragraph are recommended to be moved to the material method and explained in detail.

5. There were multiple experiments in this study, and the results of the experiments showed no significant differences. This shows that replacing fishmeal with algal biomass (Pavlova sp. 459) is ineffective.

6. In several PCO plots, this results are good and show interesting phenomena.

7. The conclusion appears to be contradictory to the abstract and the content of the results, it is recommended to confirm it.

Author Response

Thank you for your feedback, please see the attachment.

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

The authors, Guerra et al., conducted a study focused on assessing the Effects of replacing fishmeal with algal biomass strain of Pavlova sp. on the membrane lipid composition of Salmo salar parr muscle and liver tissues. I hereby endorse the submission under consideration as a scholarly article of notable scientific significance and genuine practical relevance. Consequently, I advocate the proposal's suitability for inclusion in the journal "Sustainability" (ISSN 2071-1050) following minor revisions. My comments on the manuscript are as follows:

# Abstract should be restructured to emphasize the core findings of your study and their significance. Ensure that it succinctly highlights your research and its results.

# Introduction requires further elaboration. The declaration of novelty in the research is not clearly articulated. The introduction framework should be refined, and it is crucial to emphasize the gap analysis and the need for this study before outlining the objectives.  Add a few recent references at the end of each paragraph to support the information provided. In addition, review the introduction for completeness and consider incorporating additional information such as metabolomic profiling of S.obliquus as an example. doi.org/10.1016/j.heliyon.2022.e08967 ).

Results and discussion: Please improve the analysis and interpretation of results. The discussion should be enriched. 

Conclusion: The conclusion is the same as the abstract, please rephrase it. In general, authors should carefully review the manuscript to ensure that it is free of typographical errors and unnecessary repetition.

Author Response

Thank you for your feedback, please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

Work can be interesting if authors address positive effects on immunological, microbiome and inflammatory responses instead. what were the effects on diversity of the microbiome in the fish fed with AB? How goblet cell proliferation, mucus production and inducible nitric oxide synthase activity was affected? How disease resistance was improved?

 

Pls address the above comment before accepting for publication

Author Response

Response to the reviewer #1:

1. Work can be interesting if authors address positive effects on immunological, microbiome and inflammatory responses instead. what were the effects on diversity of the microbiome in the fish fed with AB? How goblet cell proliferation, mucus production and inducible nitric oxide synthase activity was affected? How disease resistance was improved?

Thank you for recommending the inclusion of positive effects on immunological, microbiome, and inflammatory responses. In response to your suggestion, we have incorporated mentions of inflammatory responses into the manuscript on lines 726, 732, and 758. We incorporated mentions of immunology on lines 334, 336, 338, and 663-665. We incorporated mentions of the microbiome on lines 663-669. We incorporated mentions of goblet cell on lines 663-666. We incorporated mentions of mucus production on lines 663-667. And we incorporated mentions of nitric oxide on lines 663-666. We trust this revision will improve the overall quality of our manuscript.

 

Reviewer 3 Report

Comments and Suggestions for Authors

In the revised version, we have seen the author's efforts to explain and respond to reviewer comments, and make a meaningful correction. For our concerns, we can see that most of them have a new addition and improvement.

Author Response

Thank you for your positive feedback.

Round 3

Reviewer 1 Report

Comments and Suggestions for Authors

Accept

Comments on the Quality of English Language

Accept