Nutraceutical Screening in a Zebrafish Model of Muscular Dystrophy: Gingerol as a Possible Food Aid
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Care and Maintenance
2.2. Genotyping Sapje
2.3. Drugs
2.4. Birefringence Analysis
2.5. Locomotion Analysis
2.6. RNA Isolation and Real-Time PCR Analysis
2.7. Mitochondrial Respiratory Analysis
2.8. Statistical Analysis
3. Results
3.1. Analysis of Birefringence and Locomotion of Treated Sapje
3.2. Effect of Gingerol on the Hmox1 Pathway
3.3. Effect of Gingerol on Mitochondrial Function
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bioactive Compound | Concentration (μM) | Effect | Source |
---|---|---|---|
Simvastatin | 50 | Toxic | Sigma |
10 | Toxic | ||
5 | Toxic | ||
1 | Toxic | ||
Idebenone | 5 | Phenotype retained | Sigma |
1 | Phenotype retained | ||
Pterostilbene | 10 | Toxic | Sigma |
5 | Phenotype retained | ||
2 | Partial rescue | ||
1 | Phenotype retained | ||
Resveratrol | 1 | Partial rescue | Sigma |
0.5 | Phenotype retained | ||
Curcumin | 13 | Phenotype retained | Natural Products Library |
5 | Phenotype retained | ||
3 | Phenotype retained | ||
Genistein | 9 | Phenotype retained | Natural Products Library |
Daidzein | 9 | Phenotype retained | Natural Products Library |
Actinomycin D | 2 | Phenotype retained | Natural Products Library |
Gingerol | 8 | Phenotype retained | Natural Products Library |
4 | Partial rescue and increased motility | ||
Colchicine | 6 | Phenotype retained | Natural Products Library |
Cyclosporin A | 2 | Toxic | Natural Products Library |
Camptotechine | 7 | Toxic | Natural Products Library |
E64D | 7 | Phenotype retained | Natural Products Library |
4 | Phenotype retained | ||
Gossypol | 5 | Phenotype retained | Natural Products Library |
2 | Phenotype retained | ||
Epigallocatechin | 5 | Phenotype retained | Natural Products Library |
3 | Phenotype retained | ||
Quercetin | 7 | Phenotype retained | Natural Products Library |
Resveratrol | 11 | Phenotype retained | Natural Products Library |
5 | Partial rescue | ||
Shikonin | 8 | Phenotype retained | Natural Products Library |
4 | Phenotype retained | ||
Kaempferol | 8 | Phenotype retained | Natural Products Library |
4 | Phenotype retained | ||
Rottlerin | 5 | Toxic | Natural Products Library |
2 | Phenotype retained | ||
Tomatidine | 6 | Phenotype retained | Natural Products Library |
3 | Phenotype retained | ||
Ferulic Acid | 12 | Toxic | Natural Products Library |
Flavokawain A | 8 | Toxic | Natural Products Library |
4 | Partial rescue |
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Licitra, R.; Marchese, M.; Brogi, L.; Fronte, B.; Pitto, L.; Santorelli, F.M. Nutraceutical Screening in a Zebrafish Model of Muscular Dystrophy: Gingerol as a Possible Food Aid. Nutrients 2021, 13, 998. https://doi.org/10.3390/nu13030998
Licitra R, Marchese M, Brogi L, Fronte B, Pitto L, Santorelli FM. Nutraceutical Screening in a Zebrafish Model of Muscular Dystrophy: Gingerol as a Possible Food Aid. Nutrients. 2021; 13(3):998. https://doi.org/10.3390/nu13030998
Chicago/Turabian StyleLicitra, Rosario, Maria Marchese, Letizia Brogi, Baldassare Fronte, Letizia Pitto, and Filippo M. Santorelli. 2021. "Nutraceutical Screening in a Zebrafish Model of Muscular Dystrophy: Gingerol as a Possible Food Aid" Nutrients 13, no. 3: 998. https://doi.org/10.3390/nu13030998