Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis
Abstract
:1. Introduction
2. Results
2.1. Identification of the trp Expression Signature Associated with PTEC
2.2. TRPA1, TRPV2, TRPC3 and TRPC6 Expression in PCa Patients
2.3. ‘Prostate-Associated’ TRPV2 Enhances EC Viability
2.4. ‘Prostate-Associated’ TRPA1 Expression Promotes EC Migration
2.5. ‘Prostate-Associated’ TRPC3 Promotes PCa Cell Attraction
2.6. TRPA1 Promotes Vascular Network Formation and Angiogenic Sprouting both In Vitro and In Vivo
3. Discussion
4. Materials and Methods
4.1. Chemicals and Drugs
4.2. Cell Cultures and Transfection
4.3. Generation of Immortalized PTEC
4.4. Flow Cytometry
4.5. In Vivo Angiogenesis
4.6. Isolation of CD31 Positive Cells from Retinas
4.7. Patients and Tissues
4.8. Immunohistochemistry
4.9. Total RNA Extraction and Reverse Transcription
4.10. Quantitative Real-Time PCR
4.11. Western Blot
4.12. Calcium Imaging
4.13. Cell Viability and Proliferation Assay
4.14. Scratch-Wound Healing Assay
4.15. Random Migration Assay
4.16. Cell Attraction Assay
4.17. 2D Chemotaxis Assay
4.18. In Vitro Tubulogenesis
4.19. Statistical Analysis
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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TRP Channel | PCR Product Length (nt) | |
---|---|---|
Gene | NCBI Reference Sequence | |
trpv1 | NM_080704.3 | 101 |
trpv2 | NM_016113.4 | 94 |
trpv3 | NM_001258205.1 | 118 |
trpv4 | NM_021625.4 | 99 |
trpv5 | NM_019841.6 | 114 |
trpv6 | NM_018646.5 | 118 |
trpc1 | NM_001251845.1 | 118 |
trpc3 | NM_001130698.1 | 112 |
trpc4 | NM_016179.2 | 90 |
trpc5 | NM_012471.2 | 111 |
trpc6 | NM_004621.5 | 95 |
trpc7 | NM_020389.2 | 110 |
trpm1 | NM_001252020.1 | 91 |
trpm2 | NM_003307.3 | 100 |
trpm3 | NM_020952.4 | 113 |
trpm4 | NM_017636.3 | 93 |
trpm5 | NM_014555.3 | 117 |
trpm6 | NM_017662.4 | 117 |
trpm7 | NM_017672.5 | 109 |
trpm8 | NM_024080.4 | 113 |
trpa1 | NM_007332.2 | 95 |
trpp2-pkd2 | NM_000297.3 | 110 |
trpp3-pkd2l1 | NM_016112.2 | 110 |
trpml1-mcoln1 | NM_020533.2 | 117 |
trpml2-mcoln2 | NM_153259.3 | 91 |
trpml3-mcoln3 | NM_018298.10 | 96 |
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Bernardini, M.; Brossa, A.; Chinigò, G.; Grolez, G.P.; Trimaglio, G.; Allart, L.; Hulot, A.; Marot, G.; Genova, T.; Joshi, A.; et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers 2019, 11, 956. https://doi.org/10.3390/cancers11070956
Bernardini M, Brossa A, Chinigò G, Grolez GP, Trimaglio G, Allart L, Hulot A, Marot G, Genova T, Joshi A, et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers. 2019; 11(7):956. https://doi.org/10.3390/cancers11070956
Chicago/Turabian StyleBernardini, Michela, Alessia Brossa, Giorgia Chinigò, Guillaume P. Grolez, Giulia Trimaglio, Laurent Allart, Audrey Hulot, Guillemette Marot, Tullio Genova, Aditi Joshi, and et al. 2019. "Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis" Cancers 11, no. 7: 956. https://doi.org/10.3390/cancers11070956