The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle
Abstract
:1. Introduction
2. Results
2.1. Effects of TRPM7 Inhibitors on Cell Viability of WT-HEK and HEK-M7
2.2. 2-APB Inhibited TRPM7 Current and Divalent Flux
2.3. 2-APB Suppressed the Cell Proliferation in HEK-M7 but not WT-HEK Cells
2.4. 2-APB Inhibition of TRPM7 Affected the Cell Cycle but did not Promote Cell Death
2.5. 2-APB Suppressed Cell Viability in TRPM7-Expressing Breast Cancer Cell Lines
2.6. 2-APB Decreased the Overall Viability of AU565 and T47D by Inhibiting Cell Proliferation Rather Than by Promoting Cell Death
2.7. The Knock-out of TRPM7 Decreased the Divalent Influx in MDA-MB-231
2.8. The Knock-out of TRPM7 Decreased the Proliferation of WT-231 Cells
2.9. 2-APB Selectively Suppressed the Viability of WT-231 Compared with KO-231
2.10. The Viability of MDA-MB-231 Was Affected by 100 µM 2-APB by Regulating Cell Cycle rather than Cell Death (LDH and Apoptosis) and this Effect was Blocked by TRPM7 Knock-out
2.11. A Second Inhibitor, Gin Rd, Affected MDA-MB-231 Cells Similarly
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Cell Culture
4.2. Drugs and Treatments
4.3. MTT Assay
4.4. Cell Counting
4.5. Patch-Clamp Recording
4.6. Fura-2AM-Based Quench Assay
4.7. Quantitative Real Time-PCR
4.8. LDH Assay
4.9. Apoptosis Assay
4.10. Cell Cycle Flow Cytometry
4.11. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Liu, H.; Dilger, J.P.; Lin, J. The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle. Cancers 2020, 12, 131. https://doi.org/10.3390/cancers12010131
Liu H, Dilger JP, Lin J. The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle. Cancers. 2020; 12(1):131. https://doi.org/10.3390/cancers12010131
Chicago/Turabian StyleLiu, Hengrui, James P. Dilger, and Jun Lin. 2020. "The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle" Cancers 12, no. 1: 131. https://doi.org/10.3390/cancers12010131
APA StyleLiu, H., Dilger, J. P., & Lin, J. (2020). The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle. Cancers, 12(1), 131. https://doi.org/10.3390/cancers12010131