3′RNA Sequencing Accurately Classifies Formalin-Fixed Paraffin-Embedded Uterine Leiomyomas
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Principal Component Analysis Confirms Different Molecular Subtypes
2.2. Differential Expression Analysis Confirms Distinct Gene Expression Patterns
2.3. Supervised Hierarchical Clustering Accurately Classifies Leiomyomas
2.4. Exome Sequencing Reveals Biallelic Loss of FH in Two HMGA1 Overexpressing Leiomyomas
2.5. HMGA1 and PLAG1 Are Upregulated in Leiomyomas of the FH Subtype
3. Discussion
4. Materials and Methods
4.1. Study Material and Sample Selection
4.2. RNA Extraction and Sequencing
4.3. RNA Sequencing Data Analysis
4.4. Whole-Exome Sequencing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mehine, M.; Khamaiseh, S.; Ahvenainen, T.; Heikkinen, T.; Äyräväinen, A.; Pakarinen, P.; Härkki, P.; Pasanen, A.; Bützow, R.; Vahteristo, P. 3′RNA Sequencing Accurately Classifies Formalin-Fixed Paraffin-Embedded Uterine Leiomyomas. Cancers 2020, 12, 3839. https://doi.org/10.3390/cancers12123839
Mehine M, Khamaiseh S, Ahvenainen T, Heikkinen T, Äyräväinen A, Pakarinen P, Härkki P, Pasanen A, Bützow R, Vahteristo P. 3′RNA Sequencing Accurately Classifies Formalin-Fixed Paraffin-Embedded Uterine Leiomyomas. Cancers. 2020; 12(12):3839. https://doi.org/10.3390/cancers12123839
Chicago/Turabian StyleMehine, Miika, Sara Khamaiseh, Terhi Ahvenainen, Tuomas Heikkinen, Anna Äyräväinen, Päivi Pakarinen, Päivi Härkki, Annukka Pasanen, Ralf Bützow, and Pia Vahteristo. 2020. "3′RNA Sequencing Accurately Classifies Formalin-Fixed Paraffin-Embedded Uterine Leiomyomas" Cancers 12, no. 12: 3839. https://doi.org/10.3390/cancers12123839
APA StyleMehine, M., Khamaiseh, S., Ahvenainen, T., Heikkinen, T., Äyräväinen, A., Pakarinen, P., Härkki, P., Pasanen, A., Bützow, R., & Vahteristo, P. (2020). 3′RNA Sequencing Accurately Classifies Formalin-Fixed Paraffin-Embedded Uterine Leiomyomas. Cancers, 12(12), 3839. https://doi.org/10.3390/cancers12123839